Mutational analysis of the sendai virus V protein

Importance of the conserved residues for Zn binding, virus pathogenesis, and efficient RNA editing

Noriko Fukuhara, Cheng Huang, Katsuhiro Kiyotani, Tetsuya Yoshida, Takemasa Sakaguchi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The V protein of Sendai virus (SeV) is nonessential for virus replication in cell culture but indispensable for viral pathogenicity in mice. At the C terminus of the V protein, there are amino acid residues conserved among the members of the Paramyxovinae subfamily that are clustered in three regions: region I, just downstream of the RNA editing site; and regions II and III, cysteine-rich zinc-finger-like regions. In the present study, we introduced mutations into the conserved amino acids and generated nine mutant viruses. All of the viruses had impaired virus replication in mouse lungs and attenuated virulence in mice. Furthermore, the C-terminal polypeptides fused with glutathione-S-transferase with a mutation in region I, II, or III all had impaired Zn binding in a 65Zn-binding assay in solution. These results demonstrate that the conserved amino acids are important for V protein function, probably via protein conformation dependent on Zn binding. One mutant, SeV V-H318N, had inefficient RNA editing, indicating that the nucleotide that is a part of the codon encoding histidine at position 318 is conserved for the RNA editing machinery. In addition, to determine the function of the C-terminal extension of the V protein, which is not translated in recent virulent field isolates, a translational stop codon was introduced to generate the corresponding short V protein. The mutant virus showed similar virus propagation and pathogenicity, indicating that C-terminal extension of the V protein is not relevant to virus pathogenesis.

Original languageEnglish (US)
Pages (from-to)172-181
Number of pages10
JournalVirology
Volume299
Issue number2
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

RNA Editing
Virus Attachment
Viruses
Virulence
Virus Replication
Proteins
Amino Acids
Sendai virus
Protein Conformation
Mutation
Terminator Codon
Zinc Fingers
Glutathione Transferase
Histidine
Codon
Cysteine
Nucleotides
Cell Culture Techniques
Sendai virus V protein
Lung

Keywords

  • Conserved amino acid
  • Paramyxovirus
  • Pathogenicity
  • Reverse genetics
  • Sendai virus
  • V protein
  • Zn binding

ASJC Scopus subject areas

  • Virology

Cite this

Mutational analysis of the sendai virus V protein : Importance of the conserved residues for Zn binding, virus pathogenesis, and efficient RNA editing. / Fukuhara, Noriko; Huang, Cheng; Kiyotani, Katsuhiro; Yoshida, Tetsuya; Sakaguchi, Takemasa.

In: Virology, Vol. 299, No. 2, 01.01.2002, p. 172-181.

Research output: Contribution to journalArticle

Fukuhara, Noriko ; Huang, Cheng ; Kiyotani, Katsuhiro ; Yoshida, Tetsuya ; Sakaguchi, Takemasa. / Mutational analysis of the sendai virus V protein : Importance of the conserved residues for Zn binding, virus pathogenesis, and efficient RNA editing. In: Virology. 2002 ; Vol. 299, No. 2. pp. 172-181.
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