Mycobacterium bovis BCG vaccination induces memory CD4+ T cells characterized by effector biomarker expression and anti-mycobacterial activity

Janice J. Endsley; Alison Hogg; Lis J. Shell; Martin McAulay; Tracey Coffey; Chris Howard; Charles F. Capinos Scherer; W. Ray Waters; Brian Nonnecke; D. Mark Estes; Bernardo Villarreal-Ramos

doi:10.1016/j.vaccine.2007.10.011

Mycobacterium bovis BCG vaccination induces memory CD4⁺ T cells characterized by effector biomarker expression and anti-mycobacterial activity

Janice J. Endsley, Alison Hogg, Lis J. Shell, Martin McAulay, Tracey Coffey, Chris Howard, Charles F. Capinos Scherer, W. Ray Waters, Brian Nonnecke, D. Mark Estes, Bernardo Villarreal-Ramos

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The effector mechanisms used by CD4⁺ T cells to control mycobacteria differ between humans and rodent models of TB and should be investigated in additional animal models. In these studies, the bovine model was used to characterize the mycobactericidal CD4⁺ T cell response induced by vaccination with the attenuated Mycobacterium bovis bacillus Calmette-Guérin (BCG). Antigenic stimulation of peripheral blood CD4⁺ T cells from BCG-vaccinated cattle enhanced expression of perforin and IFNγ in cells expressing a CD45RA^-CD45RO⁺CD62L⁺ cell surface phenotype, enhanced transcription of granulysin, IFNγ, perforin, IL-4, IL-13, and IL-21, and enhanced anti-mycobacterial activity of CD4⁺ T cells against BCG-infected macrophages.

Original language	English (US)
Pages (from-to)	8384-8394
Number of pages	11
Journal	Vaccine
Volume	25
Issue number	50
DOIs	https://doi.org/10.1016/j.vaccine.2007.10.011
State	Published - Dec 5 2007

Keywords

CD4 T cells
Cytotoxicity
Tuberculosis

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2007.10.011

Cite this

Endsley, J. J., Hogg, A., Shell, L. J., McAulay, M., Coffey, T., Howard, C., Capinos Scherer, C. F., Waters, W. R., Nonnecke, B., Estes, D. M., & Villarreal-Ramos, B. (2007). Mycobacterium bovis BCG vaccination induces memory CD4⁺ T cells characterized by effector biomarker expression and anti-mycobacterial activity. Vaccine, 25(50), 8384-8394. https://doi.org/10.1016/j.vaccine.2007.10.011

Endsley, JJ, Hogg, A, Shell, LJ, McAulay, M, Coffey, T, Howard, C, Capinos Scherer, CF, Waters, WR, Nonnecke, B, Estes, DM & Villarreal-Ramos, B 2007, 'Mycobacterium bovis BCG vaccination induces memory CD4⁺ T cells characterized by effector biomarker expression and anti-mycobacterial activity', Vaccine, vol. 25, no. 50, pp. 8384-8394. https://doi.org/10.1016/j.vaccine.2007.10.011

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abstract = "The effector mechanisms used by CD4+ T cells to control mycobacteria differ between humans and rodent models of TB and should be investigated in additional animal models. In these studies, the bovine model was used to characterize the mycobactericidal CD4+ T cell response induced by vaccination with the attenuated Mycobacterium bovis bacillus Calmette-Gu{\'e}rin (BCG). Antigenic stimulation of peripheral blood CD4+ T cells from BCG-vaccinated cattle enhanced expression of perforin and IFNγ in cells expressing a CD45RA-CD45RO+CD62L+ cell surface phenotype, enhanced transcription of granulysin, IFNγ, perforin, IL-4, IL-13, and IL-21, and enhanced anti-mycobacterial activity of CD4+ T cells against BCG-infected macrophages.",

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author = "Endsley, {Janice J.} and Alison Hogg and Shell, {Lis J.} and Martin McAulay and Tracey Coffey and Chris Howard and {Capinos Scherer}, {Charles F.} and Waters, {W. Ray} and Brian Nonnecke and Estes, {D. Mark} and Bernardo Villarreal-Ramos",

note = "Funding Information: The authors thank the personnel of the animal facilities at IAH Compton and the National Animal Disease Center. We also thank Dan Pong for technical assistance in real-time PCR assays. We thank the TX/UK Collaborative Research Initiative for sponsoring the travel grants to initiate this effort among three laboratories in two countries. This work was supported by the NIAID Fellowship for Training in Emerging and Re-emerging Infectious Disease NIAID T32AI07536 and the Sealy Center for Vaccine Development, UTMB, USDA-NRI Project no. 2005-35204-16039 (DME and JJE), the Texas-United Kingdom Collaborative Research Initiative, and by the UK's BBSRC, DEFRA and the DTI.",

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AU - McAulay, Martin

AU - Coffey, Tracey

AU - Howard, Chris

AU - Capinos Scherer, Charles F.

AU - Waters, W. Ray

AU - Nonnecke, Brian

AU - Estes, D. Mark

AU - Villarreal-Ramos, Bernardo

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