Mycobacterium tuberculosis-specific CD8+T cell recall in convalescing TB subjects with HIV co-infection

Asiko Ongaya, Matthew Huante, Paul Mwangi, Philip Keiser, Evans Amukoye, Janice Endsley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Memory T cell populations recover following phase I chemotherapy for tuberculosis (TB) and augment the effectiveness of antibiotics during the continuation phase of treatment. For those with human immunodeficiency virus (HIV), the CD8+T cells may have an especially important role in host defense to Mycobacterium tuberculosis (M.tb) as CD4+T cell function and/or numbers decline. Here we performed a preliminary study to investigate the impact of HIV infection status on CD8+T cell effector function during the convalescent TB period. Peripheral blood samples from convalescent HIV+ and HIV- TB subjects were used to determine CD4 +T cell count and monitor antigen-specific CD8+ T cell activation of effector function (lymphoproliferation, IFN-γ, granulysin) in response to M.tb antigen. Our preliminary results suggest that HIV co-infection is associated with moderate suppression of the M.tb-specific memory CD8+T cell compartment in many subjects convalescent for TB. Interestingly, highly activated CD8+T cells were observed in recall experiments using peripheral blood from several HIV+ subjects that had low (<200 cells/mm3) CD4+T cell counts. Further investigation may provide important information for development of novel approaches to target M.tb-specific CD8+T cell memory to protect against TB in HIV-endemic regions.

Original languageEnglish
JournalTuberculosis
Volume93
Issue numberSUPPL.
DOIs
StatePublished - Dec 1 2013

Fingerprint

Virus Diseases
Coinfection
Mycobacterium tuberculosis
Tuberculosis
HIV
T-Lymphocytes
CD4 Lymphocyte Count
CD8 Antigens
Anti-Bacterial Agents
Drug Therapy

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Mycobacterium tuberculosis-specific CD8+T cell recall in convalescing TB subjects with HIV co-infection. / Ongaya, Asiko; Huante, Matthew; Mwangi, Paul; Keiser, Philip; Amukoye, Evans; Endsley, Janice.

In: Tuberculosis, Vol. 93, No. SUPPL., 01.12.2013.

Research output: Contribution to journalArticle

@article{58bfb9ce97d5476689bc2acc3d0999f0,
title = "Mycobacterium tuberculosis-specific CD8+T cell recall in convalescing TB subjects with HIV co-infection",
abstract = "Memory T cell populations recover following phase I chemotherapy for tuberculosis (TB) and augment the effectiveness of antibiotics during the continuation phase of treatment. For those with human immunodeficiency virus (HIV), the CD8+T cells may have an especially important role in host defense to Mycobacterium tuberculosis (M.tb) as CD4+T cell function and/or numbers decline. Here we performed a preliminary study to investigate the impact of HIV infection status on CD8+T cell effector function during the convalescent TB period. Peripheral blood samples from convalescent HIV+ and HIV- TB subjects were used to determine CD4 +T cell count and monitor antigen-specific CD8+ T cell activation of effector function (lymphoproliferation, IFN-γ, granulysin) in response to M.tb antigen. Our preliminary results suggest that HIV co-infection is associated with moderate suppression of the M.tb-specific memory CD8+T cell compartment in many subjects convalescent for TB. Interestingly, highly activated CD8+T cells were observed in recall experiments using peripheral blood from several HIV+ subjects that had low (<200 cells/mm3) CD4+T cell counts. Further investigation may provide important information for development of novel approaches to target M.tb-specific CD8+T cell memory to protect against TB in HIV-endemic regions.",
author = "Asiko Ongaya and Matthew Huante and Paul Mwangi and Philip Keiser and Evans Amukoye and Janice Endsley",
year = "2013",
month = "12",
day = "1",
doi = "10.1016/S1472-9792(13)70012-X",
language = "English",
volume = "93",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",
number = "SUPPL.",

}

TY - JOUR

T1 - Mycobacterium tuberculosis-specific CD8+T cell recall in convalescing TB subjects with HIV co-infection

AU - Ongaya, Asiko

AU - Huante, Matthew

AU - Mwangi, Paul

AU - Keiser, Philip

AU - Amukoye, Evans

AU - Endsley, Janice

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Memory T cell populations recover following phase I chemotherapy for tuberculosis (TB) and augment the effectiveness of antibiotics during the continuation phase of treatment. For those with human immunodeficiency virus (HIV), the CD8+T cells may have an especially important role in host defense to Mycobacterium tuberculosis (M.tb) as CD4+T cell function and/or numbers decline. Here we performed a preliminary study to investigate the impact of HIV infection status on CD8+T cell effector function during the convalescent TB period. Peripheral blood samples from convalescent HIV+ and HIV- TB subjects were used to determine CD4 +T cell count and monitor antigen-specific CD8+ T cell activation of effector function (lymphoproliferation, IFN-γ, granulysin) in response to M.tb antigen. Our preliminary results suggest that HIV co-infection is associated with moderate suppression of the M.tb-specific memory CD8+T cell compartment in many subjects convalescent for TB. Interestingly, highly activated CD8+T cells were observed in recall experiments using peripheral blood from several HIV+ subjects that had low (<200 cells/mm3) CD4+T cell counts. Further investigation may provide important information for development of novel approaches to target M.tb-specific CD8+T cell memory to protect against TB in HIV-endemic regions.

AB - Memory T cell populations recover following phase I chemotherapy for tuberculosis (TB) and augment the effectiveness of antibiotics during the continuation phase of treatment. For those with human immunodeficiency virus (HIV), the CD8+T cells may have an especially important role in host defense to Mycobacterium tuberculosis (M.tb) as CD4+T cell function and/or numbers decline. Here we performed a preliminary study to investigate the impact of HIV infection status on CD8+T cell effector function during the convalescent TB period. Peripheral blood samples from convalescent HIV+ and HIV- TB subjects were used to determine CD4 +T cell count and monitor antigen-specific CD8+ T cell activation of effector function (lymphoproliferation, IFN-γ, granulysin) in response to M.tb antigen. Our preliminary results suggest that HIV co-infection is associated with moderate suppression of the M.tb-specific memory CD8+T cell compartment in many subjects convalescent for TB. Interestingly, highly activated CD8+T cells were observed in recall experiments using peripheral blood from several HIV+ subjects that had low (<200 cells/mm3) CD4+T cell counts. Further investigation may provide important information for development of novel approaches to target M.tb-specific CD8+T cell memory to protect against TB in HIV-endemic regions.

UR - http://www.scopus.com/inward/record.url?scp=84891602188&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891602188&partnerID=8YFLogxK

U2 - 10.1016/S1472-9792(13)70012-X

DO - 10.1016/S1472-9792(13)70012-X

M3 - Article

VL - 93

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - SUPPL.

ER -