Myocardial glycogen depletion cannot explain the cardioprotective effects of ischemic preconditioning in the rat heart

Gregory K. Asimakis

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    The mechanism of ischemic preconditioning remains unknown. The role of glycogen depletion prior to prolonged ischemia was examined as a potential mechanism of ischemic preconditioning. The glycogen content of the rat heart varies in a 24-h rhythm. In a retrospective study, the relationships between the time of day the animals were sacrificed, pre-ischemic myocardial glycogen content, and post-ischemic functional recovery were assessed in non-conditioned and ischemically preconditioned hearts. The analyses were performed on previously published data . After an equilibration perfusion, isolated rat hearts were given 40 min of global ischemia followed by 30 min of reperfusion. Preconditioned hearts received 5 min of ischemia followed by a 5-min recovery period prior to the 40-min ischemic period. Some of the hearts were freeze-clamped immediately prior to the 40 min ischemic period to determine pre-ischemic glycogen content. Pre-ischemic glycogen was higher in the morning than afternoon. The time of day correlated significantly with the pre-ischemic glycogen content of non-conditioned (r = 0.67; P < 0.005) and preconditioned (r = 0.79; P < 0.001) hearts. However, time of day did not correlate significantly with post-ischemic recovery of heart rate x developed pressure (HR x DP) or end-diastolic pressure (EDP) in either the non-conditioned or preconditioned hearts. The relationships were also assessed by subdividing the groups into either morning (a.m.) or afternoon (p.m.) hearts. The pre-ischemic glycogen content was lower in the non-conditioned-p.m. (n = 5) hearts compared to the non-conditioned-a.m. (n = 10) hearts (67.6 ± 9.0 v 128.1 ± 13.3 nmol glucose/mg protein, P < 0.005). However, there were no significant differences between p.m. (n = 13) and a.m. (n = 9) non-conditioned hearts with respect to post-ischemic recovery of HR x DP (20.6 ± 4 v 12.0 ± 4% of baseline, respectively, P = N.S.). In contrast, preconditioned-p.m. (n = 6) and -a.m. (n = 7) had pre-ischemic glycogen contents of 49.6 ± 6 and 76.6 ± 5.0 nmol glucose/mg protein, respectively. These glycogen values were not significantly different from the non-conditioned-p.m. hearts (67.6 nmol/mg protein). However, post-ischemic recovery of HR x DP in the preconditioned-p.m. (n = 5) and -a.m. (n = 6) hearts were 54.6 ± 5 and 51.4 ± 8% of baseline, respectively (these values were significantly higher (P < 0.05) than the recovery for the non-conditioned-p.m. and -a.m. hearts). The results imply that the cardioprotection of ischemic preconditioning cannot be explained solely by myocardial glycogen depletion.

    Original languageEnglish (US)
    Pages (from-to)563-570
    Number of pages8
    JournalJournal of Molecular and Cellular Cardiology
    Volume28
    Issue number3
    DOIs
    StatePublished - Mar 1996

    Fingerprint

    Ischemic Preconditioning
    Glycogen
    Ischemia
    Heart Rate
    Pressure
    Glucose
    Proteins
    Reperfusion

    Keywords

    • Ischemic injury
    • Ischemic preconditioning
    • Myocardial glycogen
    • Rat heart

    ASJC Scopus subject areas

    • Molecular Biology
    • Cardiology and Cardiovascular Medicine

    Cite this

    Myocardial glycogen depletion cannot explain the cardioprotective effects of ischemic preconditioning in the rat heart. / Asimakis, Gregory K.

    In: Journal of Molecular and Cellular Cardiology, Vol. 28, No. 3, 03.1996, p. 563-570.

    Research output: Contribution to journalArticle

    @article{d0d1c8e282f2416688105fc11ac9a9b8,
    title = "Myocardial glycogen depletion cannot explain the cardioprotective effects of ischemic preconditioning in the rat heart",
    abstract = "The mechanism of ischemic preconditioning remains unknown. The role of glycogen depletion prior to prolonged ischemia was examined as a potential mechanism of ischemic preconditioning. The glycogen content of the rat heart varies in a 24-h rhythm. In a retrospective study, the relationships between the time of day the animals were sacrificed, pre-ischemic myocardial glycogen content, and post-ischemic functional recovery were assessed in non-conditioned and ischemically preconditioned hearts. The analyses were performed on previously published data . After an equilibration perfusion, isolated rat hearts were given 40 min of global ischemia followed by 30 min of reperfusion. Preconditioned hearts received 5 min of ischemia followed by a 5-min recovery period prior to the 40-min ischemic period. Some of the hearts were freeze-clamped immediately prior to the 40 min ischemic period to determine pre-ischemic glycogen content. Pre-ischemic glycogen was higher in the morning than afternoon. The time of day correlated significantly with the pre-ischemic glycogen content of non-conditioned (r = 0.67; P < 0.005) and preconditioned (r = 0.79; P < 0.001) hearts. However, time of day did not correlate significantly with post-ischemic recovery of heart rate x developed pressure (HR x DP) or end-diastolic pressure (EDP) in either the non-conditioned or preconditioned hearts. The relationships were also assessed by subdividing the groups into either morning (a.m.) or afternoon (p.m.) hearts. The pre-ischemic glycogen content was lower in the non-conditioned-p.m. (n = 5) hearts compared to the non-conditioned-a.m. (n = 10) hearts (67.6 ± 9.0 v 128.1 ± 13.3 nmol glucose/mg protein, P < 0.005). However, there were no significant differences between p.m. (n = 13) and a.m. (n = 9) non-conditioned hearts with respect to post-ischemic recovery of HR x DP (20.6 ± 4 v 12.0 ± 4{\%} of baseline, respectively, P = N.S.). In contrast, preconditioned-p.m. (n = 6) and -a.m. (n = 7) had pre-ischemic glycogen contents of 49.6 ± 6 and 76.6 ± 5.0 nmol glucose/mg protein, respectively. These glycogen values were not significantly different from the non-conditioned-p.m. hearts (67.6 nmol/mg protein). However, post-ischemic recovery of HR x DP in the preconditioned-p.m. (n = 5) and -a.m. (n = 6) hearts were 54.6 ± 5 and 51.4 ± 8{\%} of baseline, respectively (these values were significantly higher (P < 0.05) than the recovery for the non-conditioned-p.m. and -a.m. hearts). The results imply that the cardioprotection of ischemic preconditioning cannot be explained solely by myocardial glycogen depletion.",
    keywords = "Ischemic injury, Ischemic preconditioning, Myocardial glycogen, Rat heart",
    author = "Asimakis, {Gregory K.}",
    year = "1996",
    month = "3",
    doi = "10.1006/jmcc.1996.0052",
    language = "English (US)",
    volume = "28",
    pages = "563--570",
    journal = "Journal of Molecular and Cellular Cardiology",
    issn = "0022-2828",
    publisher = "Academic Press Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - Myocardial glycogen depletion cannot explain the cardioprotective effects of ischemic preconditioning in the rat heart

    AU - Asimakis, Gregory K.

    PY - 1996/3

    Y1 - 1996/3

    N2 - The mechanism of ischemic preconditioning remains unknown. The role of glycogen depletion prior to prolonged ischemia was examined as a potential mechanism of ischemic preconditioning. The glycogen content of the rat heart varies in a 24-h rhythm. In a retrospective study, the relationships between the time of day the animals were sacrificed, pre-ischemic myocardial glycogen content, and post-ischemic functional recovery were assessed in non-conditioned and ischemically preconditioned hearts. The analyses were performed on previously published data . After an equilibration perfusion, isolated rat hearts were given 40 min of global ischemia followed by 30 min of reperfusion. Preconditioned hearts received 5 min of ischemia followed by a 5-min recovery period prior to the 40-min ischemic period. Some of the hearts were freeze-clamped immediately prior to the 40 min ischemic period to determine pre-ischemic glycogen content. Pre-ischemic glycogen was higher in the morning than afternoon. The time of day correlated significantly with the pre-ischemic glycogen content of non-conditioned (r = 0.67; P < 0.005) and preconditioned (r = 0.79; P < 0.001) hearts. However, time of day did not correlate significantly with post-ischemic recovery of heart rate x developed pressure (HR x DP) or end-diastolic pressure (EDP) in either the non-conditioned or preconditioned hearts. The relationships were also assessed by subdividing the groups into either morning (a.m.) or afternoon (p.m.) hearts. The pre-ischemic glycogen content was lower in the non-conditioned-p.m. (n = 5) hearts compared to the non-conditioned-a.m. (n = 10) hearts (67.6 ± 9.0 v 128.1 ± 13.3 nmol glucose/mg protein, P < 0.005). However, there were no significant differences between p.m. (n = 13) and a.m. (n = 9) non-conditioned hearts with respect to post-ischemic recovery of HR x DP (20.6 ± 4 v 12.0 ± 4% of baseline, respectively, P = N.S.). In contrast, preconditioned-p.m. (n = 6) and -a.m. (n = 7) had pre-ischemic glycogen contents of 49.6 ± 6 and 76.6 ± 5.0 nmol glucose/mg protein, respectively. These glycogen values were not significantly different from the non-conditioned-p.m. hearts (67.6 nmol/mg protein). However, post-ischemic recovery of HR x DP in the preconditioned-p.m. (n = 5) and -a.m. (n = 6) hearts were 54.6 ± 5 and 51.4 ± 8% of baseline, respectively (these values were significantly higher (P < 0.05) than the recovery for the non-conditioned-p.m. and -a.m. hearts). The results imply that the cardioprotection of ischemic preconditioning cannot be explained solely by myocardial glycogen depletion.

    AB - The mechanism of ischemic preconditioning remains unknown. The role of glycogen depletion prior to prolonged ischemia was examined as a potential mechanism of ischemic preconditioning. The glycogen content of the rat heart varies in a 24-h rhythm. In a retrospective study, the relationships between the time of day the animals were sacrificed, pre-ischemic myocardial glycogen content, and post-ischemic functional recovery were assessed in non-conditioned and ischemically preconditioned hearts. The analyses were performed on previously published data . After an equilibration perfusion, isolated rat hearts were given 40 min of global ischemia followed by 30 min of reperfusion. Preconditioned hearts received 5 min of ischemia followed by a 5-min recovery period prior to the 40-min ischemic period. Some of the hearts were freeze-clamped immediately prior to the 40 min ischemic period to determine pre-ischemic glycogen content. Pre-ischemic glycogen was higher in the morning than afternoon. The time of day correlated significantly with the pre-ischemic glycogen content of non-conditioned (r = 0.67; P < 0.005) and preconditioned (r = 0.79; P < 0.001) hearts. However, time of day did not correlate significantly with post-ischemic recovery of heart rate x developed pressure (HR x DP) or end-diastolic pressure (EDP) in either the non-conditioned or preconditioned hearts. The relationships were also assessed by subdividing the groups into either morning (a.m.) or afternoon (p.m.) hearts. The pre-ischemic glycogen content was lower in the non-conditioned-p.m. (n = 5) hearts compared to the non-conditioned-a.m. (n = 10) hearts (67.6 ± 9.0 v 128.1 ± 13.3 nmol glucose/mg protein, P < 0.005). However, there were no significant differences between p.m. (n = 13) and a.m. (n = 9) non-conditioned hearts with respect to post-ischemic recovery of HR x DP (20.6 ± 4 v 12.0 ± 4% of baseline, respectively, P = N.S.). In contrast, preconditioned-p.m. (n = 6) and -a.m. (n = 7) had pre-ischemic glycogen contents of 49.6 ± 6 and 76.6 ± 5.0 nmol glucose/mg protein, respectively. These glycogen values were not significantly different from the non-conditioned-p.m. hearts (67.6 nmol/mg protein). However, post-ischemic recovery of HR x DP in the preconditioned-p.m. (n = 5) and -a.m. (n = 6) hearts were 54.6 ± 5 and 51.4 ± 8% of baseline, respectively (these values were significantly higher (P < 0.05) than the recovery for the non-conditioned-p.m. and -a.m. hearts). The results imply that the cardioprotection of ischemic preconditioning cannot be explained solely by myocardial glycogen depletion.

    KW - Ischemic injury

    KW - Ischemic preconditioning

    KW - Myocardial glycogen

    KW - Rat heart

    UR - http://www.scopus.com/inward/record.url?scp=0029879874&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0029879874&partnerID=8YFLogxK

    U2 - 10.1006/jmcc.1996.0052

    DO - 10.1006/jmcc.1996.0052

    M3 - Article

    C2 - 9011639

    AN - SCOPUS:0029879874

    VL - 28

    SP - 563

    EP - 570

    JO - Journal of Molecular and Cellular Cardiology

    JF - Journal of Molecular and Cellular Cardiology

    SN - 0022-2828

    IS - 3

    ER -