Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion

N. O. Alieva, A. K. Efremov, S. Hu, D. Oh, Z. Chen, M. Natarajan, H. T. Ong, A. Jégou, G. Romet-Lemonne, J. T. Groves, Michael Sheetz, J. Yan, A. D. Bershadsky

Research output: Contribution to journalArticle

Abstract

Filopodia, dynamic membrane protrusions driven by polymerization of an actin filament core, can adhere to the extracellular matrix and experience both external and cell-generated pulling forces. The role of such forces in filopodia adhesion is however insufficiently understood. Here, we study filopodia induced by overexpression of myosin X, typical for cancer cells. The lifetime of such filopodia positively correlates with the presence of myosin IIA filaments at the filopodia bases. Application of pulling forces to the filopodia tips through attached fibronectin-coated laser-trapped beads results in sustained growth of the filopodia. Pharmacological inhibition or knockdown of myosin IIA abolishes the filopodia adhesion to the beads. Formin inhibitor SMIFH2, which causes detachment of actin filaments from formin molecules, produces similar effect. Thus, centripetal force generated by myosin IIA filaments at the base of filopodium and transmitted to the tip through actin core in a formin-dependent fashion is required for filopodia adhesion.

Original languageEnglish (US)
Article number3593
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

Nonmuscle Myosin Type IIA
myosins
Pseudopodia
Actins
filaments
adhesion
Adhesion
pulling
beads
centripetal force
Myosins
Fibronectins
Polymerization
Cells
detachment
inhibitors
Membranes
Molecules
Actin Cytoskeleton
polymerization

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Alieva, N. O., Efremov, A. K., Hu, S., Oh, D., Chen, Z., Natarajan, M., ... Bershadsky, A. D. (2019). Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. Nature communications, 10(1), [3593]. https://doi.org/10.1038/s41467-019-10964-w

Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. / Alieva, N. O.; Efremov, A. K.; Hu, S.; Oh, D.; Chen, Z.; Natarajan, M.; Ong, H. T.; Jégou, A.; Romet-Lemonne, G.; Groves, J. T.; Sheetz, Michael; Yan, J.; Bershadsky, A. D.

In: Nature communications, Vol. 10, No. 1, 3593, 01.12.2019.

Research output: Contribution to journalArticle

Alieva, NO, Efremov, AK, Hu, S, Oh, D, Chen, Z, Natarajan, M, Ong, HT, Jégou, A, Romet-Lemonne, G, Groves, JT, Sheetz, M, Yan, J & Bershadsky, AD 2019, 'Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion', Nature communications, vol. 10, no. 1, 3593. https://doi.org/10.1038/s41467-019-10964-w
Alieva NO, Efremov AK, Hu S, Oh D, Chen Z, Natarajan M et al. Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. Nature communications. 2019 Dec 1;10(1). 3593. https://doi.org/10.1038/s41467-019-10964-w
Alieva, N. O. ; Efremov, A. K. ; Hu, S. ; Oh, D. ; Chen, Z. ; Natarajan, M. ; Ong, H. T. ; Jégou, A. ; Romet-Lemonne, G. ; Groves, J. T. ; Sheetz, Michael ; Yan, J. ; Bershadsky, A. D. / Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. In: Nature communications. 2019 ; Vol. 10, No. 1.
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