Myosin isoforms and contractile properties of single fibers of human latissimus dorsi muscle

Antonio Paoli, Quirico F. Pacelli, Pasqua Cancellara, Luana Toniolo, Tatiana Moro, Marta Canato, Danilo Miotti, Carlo Reggiani

Research output: Contribution to journalArticle

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Abstract

The aim of our study was to investigate fiber type distribution and contractile characteristics of Latissimus Dorsi muscle (LDM). Samples were collected from 18 young healthy subjects (9 males and 9 females) through percutaneous fine needle muscle biopsy. The results showed a predominance of fast myosin heavy chain isoforms (MyHC) with 42% of MyHC 2A and 25% of MyHC 2X, while MyHC 1 represented only 33%. The unbalance toward fast isoforms was even greater in males (71%) than in females (64%). Fiber type distribution partially reflected MyHC isoform distribution with 28% type 1/slow fibers and 5% hybrid 1/2A fibers, while fast fibers were divided into 30% type 2A, 31% type A/X, 4% type X, and 2% type 1/2X. Type 1/slow fibers were not only less abundant but also smaller in cross-sectional area than fast fibers. During maximal isometric contraction, type 1/slow fibers developed force and tension significantly lower than the two major groups of fast fibers. In conclusion, the predominance of fast fibers and their greater size and strength compared to slow fibers reveal that LDM is a muscle specialized mainly in phasic and powerful activity. Importantly, such specialization is more pronounced in males than in females.

Original languageEnglish (US)
Article number249398
JournalBioMed Research International
Volume2013
DOIs
StatePublished - 2013
Externally publishedYes

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Superficial Back Muscles
Myosins
Muscle
Myosin Heavy Chains
Protein Isoforms
Muscles
Fibers
Isometric Contraction
Fine Needle Biopsy
Healthy Volunteers
Biopsy
Needles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Paoli, A., Pacelli, Q. F., Cancellara, P., Toniolo, L., Moro, T., Canato, M., ... Reggiani, C. (2013). Myosin isoforms and contractile properties of single fibers of human latissimus dorsi muscle. BioMed Research International, 2013, [249398]. https://doi.org/10.1155/2013/249398

Myosin isoforms and contractile properties of single fibers of human latissimus dorsi muscle. / Paoli, Antonio; Pacelli, Quirico F.; Cancellara, Pasqua; Toniolo, Luana; Moro, Tatiana; Canato, Marta; Miotti, Danilo; Reggiani, Carlo.

In: BioMed Research International, Vol. 2013, 249398, 2013.

Research output: Contribution to journalArticle

Paoli, A, Pacelli, QF, Cancellara, P, Toniolo, L, Moro, T, Canato, M, Miotti, D & Reggiani, C 2013, 'Myosin isoforms and contractile properties of single fibers of human latissimus dorsi muscle', BioMed Research International, vol. 2013, 249398. https://doi.org/10.1155/2013/249398
Paoli, Antonio ; Pacelli, Quirico F. ; Cancellara, Pasqua ; Toniolo, Luana ; Moro, Tatiana ; Canato, Marta ; Miotti, Danilo ; Reggiani, Carlo. / Myosin isoforms and contractile properties of single fibers of human latissimus dorsi muscle. In: BioMed Research International. 2013 ; Vol. 2013.
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