MzrA

A novel modulator of the EnvZ/OmpR two-component regulon

Henri Gerken, Emily S. Charlson, Elisha M. Cicirelli, Linda Kenney, Rajeev Misra

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Analysis of suppressors that alleviate the acute envelope stress phenotype of a ΔbamB ΔdegP strain of Escherichia coli identified a novel protein MzrA and pleiotropic envZ mutations. Genetic evidence shows that overexpression of MzrA - formerly known as YqjB and EcfM - modulates the activity of EnvZ/OmpR similarly to pleiotropic EnvZ mutants and alter porin expression. However, porin expression in strains devoid of MzrA or overexpressing it is still sensitive to medium osmolarity, pH and procaine, all of which modulate EnvZ/OmpR activities. Thus, MzrA appears to alter the output of the EnvZ/OmpR system but not its ability to receive and respond to various environmental signals. Localization and topology experiments indicate that MzrA is a type II membrane protein, with its N-terminus exposed in the cytoplasm and C-terminus in the periplasm. Bacterial two-hybrid experiments determined that MzrA specifically interacts with EnvZ but not with OmpR or the related membrane sensor kinase, CpxA. This and additional genetic and biochemical evidence suggest that the interaction of MzrA with EnvZ would either enhance EnvZ's kinase activity or reduce its phosphatase activity, thus elevating the steady state levels of OmpR∼P. Furthermore, our data show that MzrA links the two-component envelope stress response regulators, CpxA/CpxR and EnvZ/OmpR.

Original languageEnglish (US)
Pages (from-to)1408-1422
Number of pages15
JournalMolecular Microbiology
Volume72
Issue number6
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

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Regulon
Porins
Phosphotransferases
Periplasm
Procaine
Phosphoric Monoester Hydrolases
Osmolar Concentration
Molecular Biology
Membrane Proteins
Cytoplasm
Escherichia coli
Phenotype
Mutation
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

MzrA : A novel modulator of the EnvZ/OmpR two-component regulon. / Gerken, Henri; Charlson, Emily S.; Cicirelli, Elisha M.; Kenney, Linda; Misra, Rajeev.

In: Molecular Microbiology, Vol. 72, No. 6, 01.06.2009, p. 1408-1422.

Research output: Contribution to journalArticle

Gerken, Henri ; Charlson, Emily S. ; Cicirelli, Elisha M. ; Kenney, Linda ; Misra, Rajeev. / MzrA : A novel modulator of the EnvZ/OmpR two-component regulon. In: Molecular Microbiology. 2009 ; Vol. 72, No. 6. pp. 1408-1422.
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