N-terminal analysis of normal immunoglobulin light chains-I a study of thirteen individual humans

J. A. Grant, L. Hood

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The amino acid alternatives at the amino terminal four positions were studied in the light chains from 13 normal humans. Residues characteristics of three variable region subgroup (2 kappa and 1 lambda) were present in each of the 13 individuals, implying that the corresponding genes which encode these subgroups are present in the germ line of each individual. Thus the kappa subgroup genes can not be alleles at a single structural locus. At the amino terminus the light chains from Caucasian, Mongoloid and Negroid individuals could not be distinguished from one another. Although there was a marked similarity between the hertogeneous pool of light chains from a single individual and the pool of mycloma light chains, at position 2 in normal individuals valine was seen at 2-5 times the level present in the mycloma pool. This is, to our knowledge, the first clear example of variable region selection in the myceloma process.

Original languageEnglish (US)
Pages (from-to)63-79
Number of pages17
JournalImmunochemistry
Volume8
Issue number1
DOIs
StatePublished - 1971

Fingerprint

Immunoglobulin Light Chains
Light
Valine
Germ Cells
Genes
Alleles
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

N-terminal analysis of normal immunoglobulin light chains-I a study of thirteen individual humans. / Grant, J. A.; Hood, L.

In: Immunochemistry, Vol. 8, No. 1, 1971, p. 63-79.

Research output: Contribution to journalArticle

@article{1f114e9a588141cb8f23c2a5a6b3effa,
title = "N-terminal analysis of normal immunoglobulin light chains-I a study of thirteen individual humans",
abstract = "The amino acid alternatives at the amino terminal four positions were studied in the light chains from 13 normal humans. Residues characteristics of three variable region subgroup (2 kappa and 1 lambda) were present in each of the 13 individuals, implying that the corresponding genes which encode these subgroups are present in the germ line of each individual. Thus the kappa subgroup genes can not be alleles at a single structural locus. At the amino terminus the light chains from Caucasian, Mongoloid and Negroid individuals could not be distinguished from one another. Although there was a marked similarity between the hertogeneous pool of light chains from a single individual and the pool of mycloma light chains, at position 2 in normal individuals valine was seen at 2-5 times the level present in the mycloma pool. This is, to our knowledge, the first clear example of variable region selection in the myceloma process.",
author = "Grant, {J. A.} and L. Hood",
year = "1971",
doi = "10.1016/0019-2791(71)90422-8",
language = "English (US)",
volume = "8",
pages = "63--79",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - N-terminal analysis of normal immunoglobulin light chains-I a study of thirteen individual humans

AU - Grant, J. A.

AU - Hood, L.

PY - 1971

Y1 - 1971

N2 - The amino acid alternatives at the amino terminal four positions were studied in the light chains from 13 normal humans. Residues characteristics of three variable region subgroup (2 kappa and 1 lambda) were present in each of the 13 individuals, implying that the corresponding genes which encode these subgroups are present in the germ line of each individual. Thus the kappa subgroup genes can not be alleles at a single structural locus. At the amino terminus the light chains from Caucasian, Mongoloid and Negroid individuals could not be distinguished from one another. Although there was a marked similarity between the hertogeneous pool of light chains from a single individual and the pool of mycloma light chains, at position 2 in normal individuals valine was seen at 2-5 times the level present in the mycloma pool. This is, to our knowledge, the first clear example of variable region selection in the myceloma process.

AB - The amino acid alternatives at the amino terminal four positions were studied in the light chains from 13 normal humans. Residues characteristics of three variable region subgroup (2 kappa and 1 lambda) were present in each of the 13 individuals, implying that the corresponding genes which encode these subgroups are present in the germ line of each individual. Thus the kappa subgroup genes can not be alleles at a single structural locus. At the amino terminus the light chains from Caucasian, Mongoloid and Negroid individuals could not be distinguished from one another. Although there was a marked similarity between the hertogeneous pool of light chains from a single individual and the pool of mycloma light chains, at position 2 in normal individuals valine was seen at 2-5 times the level present in the mycloma pool. This is, to our knowledge, the first clear example of variable region selection in the myceloma process.

UR - http://www.scopus.com/inward/record.url?scp=0014986089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014986089&partnerID=8YFLogxK

U2 - 10.1016/0019-2791(71)90422-8

DO - 10.1016/0019-2791(71)90422-8

M3 - Article

C2 - 4100766

AN - SCOPUS:0014986089

VL - 8

SP - 63

EP - 79

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 1

ER -