N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection

Nandan S. Gokhale, Alexa B R McIntyre, Michael J. McFadden, Allison E. Roder, Edward M. Kennedy, Jorge A. Gandara, Sharon E. Hopcraft, Kendra M. Quicke, Christine Vazquez, Jason Willer, Olga R. Ilkayeva, Brittany A. Law, Christopher L. Holley, Mariano Garcia-Blanco, Matthew J. Evans, Mehul S. Suthar, Shelton Bradrick, Christopher E. Mason, Stacy M. Horner

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The RNA modification N6-methyladenosine (m6A) post-transcriptionally regulates RNA function. The cellular machinery that controls m6A includes methyltransferases and demethylases that add or remove this modification, as well as m6A-binding YTHDF proteins that promote the translation or degradation of m6A-modified mRNA. We demonstrate that m6A modulates infection by hepatitis C virus (HCV). Depletion of m6A methyltransferases or an m6A demethylase, respectively, increases or decreases infectious HCV particle production. During HCV infection, YTHDF proteins relocalize to lipid droplets, sites of viral assembly, and their depletion increases infectious viral particles. We further mapped m6A sites across the HCV genome and determined that inactivating m6A in one viral genomic region increases viral titer without affecting RNA replication. Additional mapping of m6A on the RNA genomes of other Flaviviridae, including dengue, Zika, yellow fever, and West Nile virus, identifies conserved regions modified by m6A. Altogether, this work identifies m6A as a conserved regulatory mark across Flaviviridae genomes.

Original languageEnglish (US)
Pages (from-to)654-665
Number of pages12
JournalCell Host and Microbe
Volume20
Issue number5
DOIs
StatePublished - Nov 9 2016

Fingerprint

Flaviviridae
Viral Genome
Viral RNA
Hepacivirus
RNA
Methyltransferases
Genome
Infection
Virion
Hepatovirus
Yellow Fever
Virus Assembly
West Nile virus
Dengue
Protein Biosynthesis
Virus Diseases
Proteolysis
Carrier Proteins
Messenger RNA
Proteins

Keywords

  • dengue
  • Flaviviridae
  • HCV
  • mA
  • N6-methyladenosine
  • RNA-modifications
  • viral particle production
  • West Nile
  • yellow fever
  • Zika

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology and Microbiology(all)
  • Cancer Research

Cite this

Gokhale, N. S., McIntyre, A. B. R., McFadden, M. J., Roder, A. E., Kennedy, E. M., Gandara, J. A., ... Horner, S. M. (2016). N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host and Microbe, 20(5), 654-665. https://doi.org/10.1016/j.chom.2016.09.015

N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. / Gokhale, Nandan S.; McIntyre, Alexa B R; McFadden, Michael J.; Roder, Allison E.; Kennedy, Edward M.; Gandara, Jorge A.; Hopcraft, Sharon E.; Quicke, Kendra M.; Vazquez, Christine; Willer, Jason; Ilkayeva, Olga R.; Law, Brittany A.; Holley, Christopher L.; Garcia-Blanco, Mariano; Evans, Matthew J.; Suthar, Mehul S.; Bradrick, Shelton; Mason, Christopher E.; Horner, Stacy M.

In: Cell Host and Microbe, Vol. 20, No. 5, 09.11.2016, p. 654-665.

Research output: Contribution to journalArticle

Gokhale, NS, McIntyre, ABR, McFadden, MJ, Roder, AE, Kennedy, EM, Gandara, JA, Hopcraft, SE, Quicke, KM, Vazquez, C, Willer, J, Ilkayeva, OR, Law, BA, Holley, CL, Garcia-Blanco, M, Evans, MJ, Suthar, MS, Bradrick, S, Mason, CE & Horner, SM 2016, 'N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection', Cell Host and Microbe, vol. 20, no. 5, pp. 654-665. https://doi.org/10.1016/j.chom.2016.09.015
Gokhale NS, McIntyre ABR, McFadden MJ, Roder AE, Kennedy EM, Gandara JA et al. N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host and Microbe. 2016 Nov 9;20(5):654-665. https://doi.org/10.1016/j.chom.2016.09.015
Gokhale, Nandan S. ; McIntyre, Alexa B R ; McFadden, Michael J. ; Roder, Allison E. ; Kennedy, Edward M. ; Gandara, Jorge A. ; Hopcraft, Sharon E. ; Quicke, Kendra M. ; Vazquez, Christine ; Willer, Jason ; Ilkayeva, Olga R. ; Law, Brittany A. ; Holley, Christopher L. ; Garcia-Blanco, Mariano ; Evans, Matthew J. ; Suthar, Mehul S. ; Bradrick, Shelton ; Mason, Christopher E. ; Horner, Stacy M. / N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. In: Cell Host and Microbe. 2016 ; Vol. 20, No. 5. pp. 654-665.
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