Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma

Rinat Esenaliev, Irene Y. Petrov, Yuriy Petrov, Jutatip Guptarak, Debbie R. Boone, Emanuele Mocciaro, Harris Weisz, Margaret A. Parsley, Stacy Sell, Helen Hellmich, Jonathan M. Ford, Connor Pogue, Douglas Dewitt, Donald Prough, Maria Micci

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a novel, non-invasive nano-pulsed laser therapy (NPLT) system that combines the benefits of near-infrared laser light (808 nm) and ultrasound (optoacoustic) waves, which are generated with each short laser pulse within the tissue. We tested NPLT in a rat model of blast-induced neurotrauma (BINT) to determine whether transcranial application of NPLT provides neuroprotective effects. The laser pulses were applied on the intact rat head 1 h after injury using a specially developed fiber-optic system. Vestibulomotor function was assessed on post-injury days (PIDs) 1-3 on the beam balance and beam walking tasks. Cognitive function was assessed on PIDs 6-10 using a working memory Morris water maze (MWM) test. BDNF and caspase-3 messenger RNA (mRNA) expression was measured by quantitative real-time PCR (qRT-PCR) in laser-captured cortical neurons. Microglia activation and neuronal injury were assessed in brain sections by immunofluorescence using specific antibodies against CD68 and active caspase-3, respectively. In the vestibulomotor and cognitive (MWM) tests, NPLT-treated animals performed significantly better than the untreated blast group and similarly to sham animals. NPLT upregulated mRNA encoding BDNF and downregulated the pro-apoptotic protein caspase-3 in cortical neurons. Immunofluorescence demonstrated that NPLT inhibited microglia activation and reduced the number of cortical neurons expressing activated caspase-3. NPLT also increased expression of BDNF in the hippocampus and the number of proliferating progenitor cells in the dentate gyrus. Our data demonstrate a neuroprotective effect of NPLT and prompt further studies aimed to develop NPLT as a therapeutic intervention after traumatic brain injury (TBI).

Original languageEnglish (US)
Pages (from-to)1510-1522
Number of pages13
JournalJournal of Neurotrauma
Volume35
Issue number13
DOIs
StatePublished - Jul 1 2018

Fingerprint

Laser Therapy
Lasers
Caspase 3
Brain-Derived Neurotrophic Factor
Wounds and Injuries
Microglia
Neuroprotective Agents
Neurons
Fluorescent Antibody Technique
Messenger RNA
Apoptosis Regulatory Proteins
Water
Dentate Gyrus
Short-Term Memory
Cognition
Walking
Real-Time Polymerase Chain Reaction
Hippocampus
Stem Cells
Down-Regulation

Keywords

  • Blast injury
  • Near-infrared light
  • Neuro protection
  • Non-invasive transcranial laser therapy
  • Optoacoustics
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma. / Esenaliev, Rinat; Petrov, Irene Y.; Petrov, Yuriy; Guptarak, Jutatip; Boone, Debbie R.; Mocciaro, Emanuele; Weisz, Harris; Parsley, Margaret A.; Sell, Stacy; Hellmich, Helen; Ford, Jonathan M.; Pogue, Connor; Dewitt, Douglas; Prough, Donald; Micci, Maria.

In: Journal of Neurotrauma, Vol. 35, No. 13, 01.07.2018, p. 1510-1522.

Research output: Contribution to journalArticle

Esenaliev, R, Petrov, IY, Petrov, Y, Guptarak, J, Boone, DR, Mocciaro, E, Weisz, H, Parsley, MA, Sell, S, Hellmich, H, Ford, JM, Pogue, C, Dewitt, D, Prough, D & Micci, M 2018, 'Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma', Journal of Neurotrauma, vol. 35, no. 13, pp. 1510-1522. https://doi.org/10.1089/neu.2017.5249
Esenaliev, Rinat ; Petrov, Irene Y. ; Petrov, Yuriy ; Guptarak, Jutatip ; Boone, Debbie R. ; Mocciaro, Emanuele ; Weisz, Harris ; Parsley, Margaret A. ; Sell, Stacy ; Hellmich, Helen ; Ford, Jonathan M. ; Pogue, Connor ; Dewitt, Douglas ; Prough, Donald ; Micci, Maria. / Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma. In: Journal of Neurotrauma. 2018 ; Vol. 35, No. 13. pp. 1510-1522.
@article{c8847876a06448faa42ea9e1c1be9730,
title = "Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma",
abstract = "We have developed a novel, non-invasive nano-pulsed laser therapy (NPLT) system that combines the benefits of near-infrared laser light (808 nm) and ultrasound (optoacoustic) waves, which are generated with each short laser pulse within the tissue. We tested NPLT in a rat model of blast-induced neurotrauma (BINT) to determine whether transcranial application of NPLT provides neuroprotective effects. The laser pulses were applied on the intact rat head 1 h after injury using a specially developed fiber-optic system. Vestibulomotor function was assessed on post-injury days (PIDs) 1-3 on the beam balance and beam walking tasks. Cognitive function was assessed on PIDs 6-10 using a working memory Morris water maze (MWM) test. BDNF and caspase-3 messenger RNA (mRNA) expression was measured by quantitative real-time PCR (qRT-PCR) in laser-captured cortical neurons. Microglia activation and neuronal injury were assessed in brain sections by immunofluorescence using specific antibodies against CD68 and active caspase-3, respectively. In the vestibulomotor and cognitive (MWM) tests, NPLT-treated animals performed significantly better than the untreated blast group and similarly to sham animals. NPLT upregulated mRNA encoding BDNF and downregulated the pro-apoptotic protein caspase-3 in cortical neurons. Immunofluorescence demonstrated that NPLT inhibited microglia activation and reduced the number of cortical neurons expressing activated caspase-3. NPLT also increased expression of BDNF in the hippocampus and the number of proliferating progenitor cells in the dentate gyrus. Our data demonstrate a neuroprotective effect of NPLT and prompt further studies aimed to develop NPLT as a therapeutic intervention after traumatic brain injury (TBI).",
keywords = "Blast injury, Near-infrared light, Neuro protection, Non-invasive transcranial laser therapy, Optoacoustics, Traumatic brain injury",
author = "Rinat Esenaliev and Petrov, {Irene Y.} and Yuriy Petrov and Jutatip Guptarak and Boone, {Debbie R.} and Emanuele Mocciaro and Harris Weisz and Parsley, {Margaret A.} and Stacy Sell and Helen Hellmich and Ford, {Jonathan M.} and Connor Pogue and Douglas Dewitt and Donald Prough and Maria Micci",
year = "2018",
month = "7",
day = "1",
doi = "10.1089/neu.2017.5249",
language = "English (US)",
volume = "35",
pages = "1510--1522",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "13",

}

TY - JOUR

T1 - Nano-Pulsed Laser Therapy Is Neuroprotective in a Rat Model of Blast-Induced Neurotrauma

AU - Esenaliev, Rinat

AU - Petrov, Irene Y.

AU - Petrov, Yuriy

AU - Guptarak, Jutatip

AU - Boone, Debbie R.

AU - Mocciaro, Emanuele

AU - Weisz, Harris

AU - Parsley, Margaret A.

AU - Sell, Stacy

AU - Hellmich, Helen

AU - Ford, Jonathan M.

AU - Pogue, Connor

AU - Dewitt, Douglas

AU - Prough, Donald

AU - Micci, Maria

PY - 2018/7/1

Y1 - 2018/7/1

N2 - We have developed a novel, non-invasive nano-pulsed laser therapy (NPLT) system that combines the benefits of near-infrared laser light (808 nm) and ultrasound (optoacoustic) waves, which are generated with each short laser pulse within the tissue. We tested NPLT in a rat model of blast-induced neurotrauma (BINT) to determine whether transcranial application of NPLT provides neuroprotective effects. The laser pulses were applied on the intact rat head 1 h after injury using a specially developed fiber-optic system. Vestibulomotor function was assessed on post-injury days (PIDs) 1-3 on the beam balance and beam walking tasks. Cognitive function was assessed on PIDs 6-10 using a working memory Morris water maze (MWM) test. BDNF and caspase-3 messenger RNA (mRNA) expression was measured by quantitative real-time PCR (qRT-PCR) in laser-captured cortical neurons. Microglia activation and neuronal injury were assessed in brain sections by immunofluorescence using specific antibodies against CD68 and active caspase-3, respectively. In the vestibulomotor and cognitive (MWM) tests, NPLT-treated animals performed significantly better than the untreated blast group and similarly to sham animals. NPLT upregulated mRNA encoding BDNF and downregulated the pro-apoptotic protein caspase-3 in cortical neurons. Immunofluorescence demonstrated that NPLT inhibited microglia activation and reduced the number of cortical neurons expressing activated caspase-3. NPLT also increased expression of BDNF in the hippocampus and the number of proliferating progenitor cells in the dentate gyrus. Our data demonstrate a neuroprotective effect of NPLT and prompt further studies aimed to develop NPLT as a therapeutic intervention after traumatic brain injury (TBI).

AB - We have developed a novel, non-invasive nano-pulsed laser therapy (NPLT) system that combines the benefits of near-infrared laser light (808 nm) and ultrasound (optoacoustic) waves, which are generated with each short laser pulse within the tissue. We tested NPLT in a rat model of blast-induced neurotrauma (BINT) to determine whether transcranial application of NPLT provides neuroprotective effects. The laser pulses were applied on the intact rat head 1 h after injury using a specially developed fiber-optic system. Vestibulomotor function was assessed on post-injury days (PIDs) 1-3 on the beam balance and beam walking tasks. Cognitive function was assessed on PIDs 6-10 using a working memory Morris water maze (MWM) test. BDNF and caspase-3 messenger RNA (mRNA) expression was measured by quantitative real-time PCR (qRT-PCR) in laser-captured cortical neurons. Microglia activation and neuronal injury were assessed in brain sections by immunofluorescence using specific antibodies against CD68 and active caspase-3, respectively. In the vestibulomotor and cognitive (MWM) tests, NPLT-treated animals performed significantly better than the untreated blast group and similarly to sham animals. NPLT upregulated mRNA encoding BDNF and downregulated the pro-apoptotic protein caspase-3 in cortical neurons. Immunofluorescence demonstrated that NPLT inhibited microglia activation and reduced the number of cortical neurons expressing activated caspase-3. NPLT also increased expression of BDNF in the hippocampus and the number of proliferating progenitor cells in the dentate gyrus. Our data demonstrate a neuroprotective effect of NPLT and prompt further studies aimed to develop NPLT as a therapeutic intervention after traumatic brain injury (TBI).

KW - Blast injury

KW - Near-infrared light

KW - Neuro protection

KW - Non-invasive transcranial laser therapy

KW - Optoacoustics

KW - Traumatic brain injury

UR - http://www.scopus.com/inward/record.url?scp=85047354472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047354472&partnerID=8YFLogxK

U2 - 10.1089/neu.2017.5249

DO - 10.1089/neu.2017.5249

M3 - Article

VL - 35

SP - 1510

EP - 1522

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 13

ER -