Nanometer measurements of motor protein and membrane glycoprotein movements

Michael Sheetz, J. Gelles, E. R. Steuer, B. J. Schnapp, J. Dubinska, H. Qian, E. Elson

Research output: Contribution to journalArticle

Abstract

The recent application of digital imaging technologies to the analysis of video images from a DIC (differential interference contrast) light microscope has given 1-2 nm precision for the measurement of movements of submicron particles at 30 Hz. [Gelles et al., Nature 331(1988)450]. This technique has been applied to the study of the movements of 1) motor proteins (kinesin and cytoplasmic dynein) on microtubules (MTs) and 2) membrane glycoproteins in cellular plasma membranes. In both cases the nanometer measurements have provided important new insights into the molecular mechanisms which govern those biological movements.

Original languageEnglish (US)
Pages (from-to)264-265
Number of pages2
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1161
DOIs
StatePublished - Dec 22 1989
Externally publishedYes

Fingerprint

Glycoproteins
Glycoprotein
Membrane Glycoproteins
Membrane
Cytoplasmic Dyneins
membranes
proteins
Proteins
Membranes
Protein
Kinesin
Cell membranes
Digital Imaging
Microtubules
Microscopes
Plasma Membrane
Imaging techniques
Microscope
Interference
microscopes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Nanometer measurements of motor protein and membrane glycoprotein movements. / Sheetz, Michael; Gelles, J.; Steuer, E. R.; Schnapp, B. J.; Dubinska, J.; Qian, H.; Elson, E.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1161, 22.12.1989, p. 264-265.

Research output: Contribution to journalArticle

Sheetz, Michael ; Gelles, J. ; Steuer, E. R. ; Schnapp, B. J. ; Dubinska, J. ; Qian, H. ; Elson, E. / Nanometer measurements of motor protein and membrane glycoprotein movements. In: Proceedings of SPIE - The International Society for Optical Engineering. 1989 ; Vol. 1161. pp. 264-265.
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