TY - JOUR
T1 - Nanoparticle-encapsulated antioxidant improves placental mitochondrial function in a sexually dimorphic manner in a rat model of prenatal hypoxia
AU - Ganguly, Esha
AU - Kirschenman, Raven
AU - Spaans, Floor
AU - Holody, Claudia D.
AU - Phillips, Thomas E.J.
AU - Case, C. Patrick
AU - Cooke, Christy Lynn M.
AU - Murphy, Michael P.
AU - Lemieux, Hélène
AU - Davidge, Sandra T.
N1 - Funding Information:
This research was funded by grants from the Canadian Institutes of Health Research (CIHR FS 154313) and the Women and Children’s Health Research Institute (WCHRI) through the generosity of the Stollery Children’s Hospital Foundation and supporters of the Lois Hole Hospital for Women supporting the laboratory of SD. SD is a Canada Research Chair in Maternal and Perinatal Cardiovascular Health and a Distinguished University Professor at the University of Alberta. HL was supported by a discovery grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN 402636). EG was supported by Faculty of Medicine & Dentistry 75 Anniversary Awards, WCHRI Graduate Studentship Program and Dean’s Doctoral Award from the University of Alberta. th
Funding Information:
This research was funded by grants from the Canadian Institutes of Health Research (CIHR?FS 154313) and the Women and Children?s Health Research Institute (WCHRI) through the generosity of the Stollery Children?s Hospital Foundation and supporters of the Lois Hole Hospital for Women supporting the laboratory of SD. SD is a Canada Research Chair in Maternal and Perinatal Cardiovascular Health and a Distinguished University Professor at the University of Alberta. HL was supported by a discovery grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN 402636). EG was supported by Faculty of Medicine & Dentistry 75th Anniversary Awards, WCHRI Graduate Studentship Program and Dean?s Doctoral Award from the University of Alberta. The authors acknowledge Ted Han for helping E.G and R.K setup the respirometry experiments.
Publisher Copyright:
© 2021 Federation of American Societies for Experimental Biology.
PY - 2021/2
Y1 - 2021/2
N2 - Pregnancy complications associated with prenatal hypoxia lead to increased placental oxidative stress. Previous studies suggest that prenatal hypoxia can reduce mitochondrial respiratory capacity and mitochondrial fusion, which could lead to placental dysfunction and impaired fetal development. We developed a placenta-targeted treatment strategy using a mitochondrial antioxidant, MitoQ, encapsulated into nanoparticles (nMitoQ) to reduce placental oxidative stress and (indirectly) improve fetal outcomes. We hypothesized that, in a rat model of prenatal hypoxia, nMitoQ improves placental mitochondrial function and promotes mitochondrial fusion in both male and female placentae. Pregnant rats were treated with saline or nMitoQ on gestational day (GD) 15 and exposed to normoxia (21% O2) or hypoxia (11% O2) from GD15-21. On GD21, male and female placental labyrinth zones were collected for mitochondrial respirometry assessments, mitochondrial content, and markers of mitochondrial biogenesis, fusion and fission. Prenatal hypoxia reduced complex IV activity and fusion in male placentae, while nMitoQ improved complex IV activity in hypoxic male placentae. In female placentae, prenatal hypoxia decreased respiration through the S-pathway (complex II) and increased N-pathway (complex I) respiration, while nMitoQ increased fusion in hypoxic female placentae. No changes in mitochondrial content, biogenesis or fission were found. In conclusion, nMitoQ improved placental mitochondrial function in male and female placentae from fetuses exposed to prenatal hypoxia, which may contribute to improved placental function. However, the mechanisms (ie, changes in mitochondrial respiratory capacity and mitochondrial fusion) were distinct between the sexes. Treatment strategies targeted against placental oxidative stress could improve placental mitochondrial function in complicated pregnancies.
AB - Pregnancy complications associated with prenatal hypoxia lead to increased placental oxidative stress. Previous studies suggest that prenatal hypoxia can reduce mitochondrial respiratory capacity and mitochondrial fusion, which could lead to placental dysfunction and impaired fetal development. We developed a placenta-targeted treatment strategy using a mitochondrial antioxidant, MitoQ, encapsulated into nanoparticles (nMitoQ) to reduce placental oxidative stress and (indirectly) improve fetal outcomes. We hypothesized that, in a rat model of prenatal hypoxia, nMitoQ improves placental mitochondrial function and promotes mitochondrial fusion in both male and female placentae. Pregnant rats were treated with saline or nMitoQ on gestational day (GD) 15 and exposed to normoxia (21% O2) or hypoxia (11% O2) from GD15-21. On GD21, male and female placental labyrinth zones were collected for mitochondrial respirometry assessments, mitochondrial content, and markers of mitochondrial biogenesis, fusion and fission. Prenatal hypoxia reduced complex IV activity and fusion in male placentae, while nMitoQ improved complex IV activity in hypoxic male placentae. In female placentae, prenatal hypoxia decreased respiration through the S-pathway (complex II) and increased N-pathway (complex I) respiration, while nMitoQ increased fusion in hypoxic female placentae. No changes in mitochondrial content, biogenesis or fission were found. In conclusion, nMitoQ improved placental mitochondrial function in male and female placentae from fetuses exposed to prenatal hypoxia, which may contribute to improved placental function. However, the mechanisms (ie, changes in mitochondrial respiratory capacity and mitochondrial fusion) were distinct between the sexes. Treatment strategies targeted against placental oxidative stress could improve placental mitochondrial function in complicated pregnancies.
KW - mitochondrial function
KW - nMitoQ
KW - placenta
KW - prenatal hypoxia
UR - http://www.scopus.com/inward/record.url?scp=85099082393&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099082393&partnerID=8YFLogxK
U2 - 10.1096/fj.202002193R
DO - 10.1096/fj.202002193R
M3 - Article
C2 - 33428278
AN - SCOPUS:85099082393
SN - 0892-6638
VL - 35
JO - FASEB Journal
JF - FASEB Journal
IS - 2
M1 - e21338
ER -