TY - JOUR
T1 - Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections
AU - Versiani, Alice F.
AU - Martins, Estefânia M.N.
AU - Andrade, Lidia M.
AU - Cox, Laura
AU - Pereira, Glauco C.
AU - Barbosa-Stancioli, Edel F.
AU - Nogueira, Mauricio L.
AU - Ladeira, Luiz O.
AU - da Fonseca, Flávio G.
N1 - Funding Information:
We appreciate the scientific and technical support provided by R Freitas (Chemistry Department/UFMG), GS Trindade (Microbiology Department/UFMG), BM Silva (Department of Biological Sciences/UFOP), LV Cambraia (CTNano/UFMG), and EG Kroon (Microbiology Department/UFMG). We thank CA Furtado and AP Santos from the Centro de Desenvolvimento da Tecnologia Nuclear (CDTN) for encouraging the development of this project and for access to equipment and materials. We also thank FV de Oliveira for the artwork contributions. This work was supported by CAPES, FAPEMIG, FINEP and CNPq. Grants from FAPEMIG were CBB-APQ-03081-17 (EDITAL 001/2017—DEMANDA UNIVERSAL), CBB-APQ-04295-17 (EDITAL 007/2017—PROGRAMA DE PESQUISA PARA O SUS/PPSUS) and Rede Mineira de Pesquisa e Inovação para Bioengenharia de Nanossistemas (RM PI-BEM—FAPEMIG-TEC-RED-00282-16). A FINEP-ZIKA institutional grant was used in the development of this work. EF Barbosa-Stancioli, ML Nogueira, LO Ladeira and FG da Fonseca are CNPq fellowship recipients. ML Nogueira is supported by FAPESP (grant #13/21719-3). AFV was a CAPES Doctorate fellow and is currently a FAPESP fellow. Other than funding, sponsoring agencies had no role on the study design, collection and interpretation of the data, and writing of the manuscript. Two patents on the described results were filed: file BR 10 2013 033868 at the Instituto Nacional de Propriedade Intelectual (INPI, Brazil) and file 055498 at the World Intellectual Property Organization (WIPO—Geneva, Switzerland).
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices.
AB - The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices.
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U2 - 10.1038/s41598-020-68357-9
DO - 10.1038/s41598-020-68357-9
M3 - Article
C2 - 32647259
AN - SCOPUS:85087803313
SN - 2045-2322
VL - 10
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 11302
ER -