TY - JOUR
T1 - Nanozyme-strip for rapid local diagnosis of Ebola
AU - Duan, Demin
AU - Fan, Kelong
AU - Zhang, Dexi
AU - Tan, Shuguang
AU - Liang, Mifang
AU - Liu, Yang
AU - Zhang, Jianlin
AU - Zhang, Panhe
AU - Liu, Wei
AU - Qiu, Xiangguo
AU - Kobinger, Gary P.
AU - Fu Gao, George
AU - Yan, Xiyun
N1 - Funding Information:
The authors thank Prof. Sarah Perrett and Minmin Liang for editing this manuscript and Dr. Lizeng Gao for assistance with the preparation of MNPs. This work was supported in part by grants from the National Science and Technology Major Project ( 2013ZX10004102 , 2012ZX10002009-016 ), Strategic Priority Research Program of the Chinese Academy of Sciences ( XDA09030306 ), National Natural Science Foundation of China ( 31270908 , 81201698 ), 973 Program ( 2011CB933503 , 2012CB934003 ), the Knowledge Innovation Program of the Chinese Academy of Sciences ( CXJJ-14-M24 ) and the special project of Ebola virus research from the president foundation of Chinese Academy of Sciences .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/12/5
Y1 - 2015/12/5
N2 - Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas.
AB - Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas.
KW - Ebola detection
KW - Immunochromatographic strip
KW - Nanozyme
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U2 - 10.1016/j.bios.2015.05.025
DO - 10.1016/j.bios.2015.05.025
M3 - Article
C2 - 26134291
AN - SCOPUS:84934273894
SN - 0956-5663
VL - 74
SP - 134
EP - 141
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -