Abstract
Integrin adhesions assemble and mature in response to ligand binding and mechanical factors, but the molecular-level organization is not known. We report that ~100-nm clusters of ~50 β3-activated integrins form very early adhesions under a wide variety of conditions on RGD surfaces. These adhesions form similarly on fluid and rigid substrates, but most adhesions are transient on rigid substrates. Without talin or actin polymerization, few early adhesions form, but expression of either the talin head or rod domain in talin-depleted cells restores early adhesion formation. Mutation of the integrin binding site in the talin rod decreases cluster size. We suggest that the integrin clusters constitute universal early adhesions and that they are the modular units of cell matrix adhesions. They require the association of activated integrins with cytoplasmic proteins, in particular talin and actin, and cytoskeletal contraction on them causes adhesion maturation for cell motility and growth.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 614-621 |
| Number of pages | 8 |
| Journal | Developmental Cell |
| Volume | 35 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2015 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Developmental Biology
Cite this
Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation. / Changede, Rishita; Xu, Xiaochun; Margadant, Felix; Sheetz, Michael.
In: Developmental Cell, Vol. 35, No. 5, 01.01.2015, p. 614-621.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation
AU - Changede, Rishita
AU - Xu, Xiaochun
AU - Margadant, Felix
AU - Sheetz, Michael
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Integrin adhesions assemble and mature in response to ligand binding and mechanical factors, but the molecular-level organization is not known. We report that ~100-nm clusters of ~50 β3-activated integrins form very early adhesions under a wide variety of conditions on RGD surfaces. These adhesions form similarly on fluid and rigid substrates, but most adhesions are transient on rigid substrates. Without talin or actin polymerization, few early adhesions form, but expression of either the talin head or rod domain in talin-depleted cells restores early adhesion formation. Mutation of the integrin binding site in the talin rod decreases cluster size. We suggest that the integrin clusters constitute universal early adhesions and that they are the modular units of cell matrix adhesions. They require the association of activated integrins with cytoplasmic proteins, in particular talin and actin, and cytoskeletal contraction on them causes adhesion maturation for cell motility and growth.
AB - Integrin adhesions assemble and mature in response to ligand binding and mechanical factors, but the molecular-level organization is not known. We report that ~100-nm clusters of ~50 β3-activated integrins form very early adhesions under a wide variety of conditions on RGD surfaces. These adhesions form similarly on fluid and rigid substrates, but most adhesions are transient on rigid substrates. Without talin or actin polymerization, few early adhesions form, but expression of either the talin head or rod domain in talin-depleted cells restores early adhesion formation. Mutation of the integrin binding site in the talin rod decreases cluster size. We suggest that the integrin clusters constitute universal early adhesions and that they are the modular units of cell matrix adhesions. They require the association of activated integrins with cytoplasmic proteins, in particular talin and actin, and cytoskeletal contraction on them causes adhesion maturation for cell motility and growth.
UR - http://www.scopus.com/inward/record.url?scp=84949591113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949591113&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2015.11.001
DO - 10.1016/j.devcel.2015.11.001
M3 - Article
VL - 35
SP - 614
EP - 621
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 5
ER -