Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation

Rishita Changede, Xiaochun Xu, Felix Margadant, Michael Sheetz

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Integrin adhesions assemble and mature in response to ligand binding and mechanical factors, but the molecular-level organization is not known. We report that ~100-nm clusters of ~50 β3-activated integrins form very early adhesions under a wide variety of conditions on RGD surfaces. These adhesions form similarly on fluid and rigid substrates, but most adhesions are transient on rigid substrates. Without talin or actin polymerization, few early adhesions form, but expression of either the talin head or rod domain in talin-depleted cells restores early adhesion formation. Mutation of the integrin binding site in the talin rod decreases cluster size. We suggest that the integrin clusters constitute universal early adhesions and that they are the modular units of cell matrix adhesions. They require the association of activated integrins with cytoplasmic proteins, in particular talin and actin, and cytoskeletal contraction on them causes adhesion maturation for cell motility and growth.

Original languageEnglish (US)
Pages (from-to)614-621
Number of pages8
JournalDevelopmental Cell
Volume35
Issue number5
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Talin
Integrins
Rigidity
Adhesion
Chemical activation
Actins
Cell-Matrix Junctions
Polymerization
Cell Movement
Binding Sites
Ligands
Mutation
Substrates
Growth
Proteins
Association reactions

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation. / Changede, Rishita; Xu, Xiaochun; Margadant, Felix; Sheetz, Michael.

In: Developmental Cell, Vol. 35, No. 5, 01.01.2015, p. 614-621.

Research output: Contribution to journalArticle

Changede, Rishita ; Xu, Xiaochun ; Margadant, Felix ; Sheetz, Michael. / Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation. In: Developmental Cell. 2015 ; Vol. 35, No. 5. pp. 614-621.
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