Nasopharyngeal acute phase cytokines in viral upper respiratory infection

Impact on acute otitis media in children

Janak Patel, Sangeeta Nair, Krystal Revai, James Grady, Tasnee Chonmaitree

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

BACKGROUND:: The role of acute phase cytokines generated in the nasopharynx during viral upper respiratory infection (URI) in subsequent development of acute otitis media (AOM) has not been examined. METHODS:: We studied 326 virus-positive URI episodes in 151 children aged 6-36 months. Nasopharyngeal secretions collected within 1 to 7 days of URI onset were studied for viruses by conventional and molecular techniques, and for concentrations of IL-1β, IL-6, and TNFα by multiplex enzyme-linked immunosorbent assay. Children were followed up for 28 days to document AOM complication. RESULTS:: IL-1β, IL-6, and TNFα concentrations correlated positively with each other (P < 0.001). IL-6 and TNFα concentrations were higher in males than in females (P = 0.01 and 0.02). IL-6 and TNFα concentrations were inversely correlated with age (P = 0.02 and 0.05). IL-6 concentrations correlated positively with duration of fever (P = 0.006) and correlated negatively with the number of days of URI symptoms (P = 0.026). Furthermore, IL-6 concentrations were significantly higher during adenovirus and influenza virus URIs as compared with enterovirus and rhinovirus URIs (P < 0.01). IL-1β concentrations were higher during URI episodes with AOM than those without AOM (P < 0.001). CONCLUSIONS:: We found IL-6 nasopharyngeal secretions concentrations to be higher with adenovirus and influenza infection, and in children with systemic febrile response during URI. However, IL-1β was found to play a more important role in the development of AOM after URI. Additional studies are needed to further define the role of acute phase cytokines in virus-induced AOM.

Original languageEnglish (US)
Pages (from-to)1002-1007
Number of pages6
JournalPediatric Infectious Disease Journal
Volume28
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Otitis Media
Respiratory Tract Infections
Interleukin-6
Cytokines
Interleukin-1
Viruses
Fever
Adenoviridae Infections
Rhinovirus
Nasopharynx
Enterovirus
Orthomyxoviridae
Adenoviridae
Human Influenza
Enzyme-Linked Immunosorbent Assay

Keywords

  • Acute otitis media
  • Acute phase cytokines
  • Upper respiratory tract infection

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Nasopharyngeal acute phase cytokines in viral upper respiratory infection : Impact on acute otitis media in children. / Patel, Janak; Nair, Sangeeta; Revai, Krystal; Grady, James; Chonmaitree, Tasnee.

In: Pediatric Infectious Disease Journal, Vol. 28, No. 11, 11.2009, p. 1002-1007.

Research output: Contribution to journalArticle

Patel, Janak ; Nair, Sangeeta ; Revai, Krystal ; Grady, James ; Chonmaitree, Tasnee. / Nasopharyngeal acute phase cytokines in viral upper respiratory infection : Impact on acute otitis media in children. In: Pediatric Infectious Disease Journal. 2009 ; Vol. 28, No. 11. pp. 1002-1007.
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abstract = "BACKGROUND:: The role of acute phase cytokines generated in the nasopharynx during viral upper respiratory infection (URI) in subsequent development of acute otitis media (AOM) has not been examined. METHODS:: We studied 326 virus-positive URI episodes in 151 children aged 6-36 months. Nasopharyngeal secretions collected within 1 to 7 days of URI onset were studied for viruses by conventional and molecular techniques, and for concentrations of IL-1β, IL-6, and TNFα by multiplex enzyme-linked immunosorbent assay. Children were followed up for 28 days to document AOM complication. RESULTS:: IL-1β, IL-6, and TNFα concentrations correlated positively with each other (P < 0.001). IL-6 and TNFα concentrations were higher in males than in females (P = 0.01 and 0.02). IL-6 and TNFα concentrations were inversely correlated with age (P = 0.02 and 0.05). IL-6 concentrations correlated positively with duration of fever (P = 0.006) and correlated negatively with the number of days of URI symptoms (P = 0.026). Furthermore, IL-6 concentrations were significantly higher during adenovirus and influenza virus URIs as compared with enterovirus and rhinovirus URIs (P < 0.01). IL-1β concentrations were higher during URI episodes with AOM than those without AOM (P < 0.001). CONCLUSIONS:: We found IL-6 nasopharyngeal secretions concentrations to be higher with adenovirus and influenza infection, and in children with systemic febrile response during URI. However, IL-1β was found to play a more important role in the development of AOM after URI. Additional studies are needed to further define the role of acute phase cytokines in virus-induced AOM.",
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