Nasopharyngeal carcinoma in children-A retrospective review and demonstration of epstein-barr viral genomes in tumor cell cytoplasm: A report of the pediatric oncology group

Edith P. Hawkins, Jeffrey P. Krischer, Billie E. Smith, Hal K. Hawkins, Milton J. Finegold

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Twenty-seven nasopharyngeal carcinomas were entered in the Pediatric Oncology Group Rare Tumor Registry from 1973 to 1988 (15 males, 12 females; 10 white, 15 black, two unknown; ages 8 to 17 years). Eight tumors were non-keratinizing carcinomas (World Health Organization 2) and 19 were undifferentiated (World Health Organization 3). The overall 3-year survival rate was 70% (SE 11%). Nine children developed distant metastases and two were salvaged. We found that localized tumor (P = .02) and black race (P = .05) were associated with a better outcome. In situ hybridization using a biotinylated probe demonstrated Epstein-Barr virus DNA in the cytoplasm of the neoplastic epithelial cells in nine of 11 tumors examined, firmly establishing the presence of Epstein-Barr virus within the malignant cells of nasopharyngeal carcinomas of both World Health Organization 2 and World Health Organization 3 histology, rather than in the surrounding lymphocytes.

Original languageEnglish (US)
Pages (from-to)805-810
Number of pages6
JournalHuman Pathology
Volume21
Issue number8
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Viral Genome
Cytoplasm
Pediatrics
Human Herpesvirus 4
Neoplasms
In Situ Hybridization
Registries
Histology
Survival Rate
Epithelial Cells
Lymphocytes
Neoplasm Metastasis
Carcinoma
Nasopharyngeal carcinoma
DNA

Keywords

  • children
  • EBV-DNA
  • histochemical in situ hybridization
  • nasopharyngeal carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Nasopharyngeal carcinoma in children-A retrospective review and demonstration of epstein-barr viral genomes in tumor cell cytoplasm : A report of the pediatric oncology group. / Hawkins, Edith P.; Krischer, Jeffrey P.; Smith, Billie E.; Hawkins, Hal K.; Finegold, Milton J.

In: Human Pathology, Vol. 21, No. 8, 1990, p. 805-810.

Research output: Contribution to journalArticle

Hawkins, Edith P. ; Krischer, Jeffrey P. ; Smith, Billie E. ; Hawkins, Hal K. ; Finegold, Milton J. / Nasopharyngeal carcinoma in children-A retrospective review and demonstration of epstein-barr viral genomes in tumor cell cytoplasm : A report of the pediatric oncology group. In: Human Pathology. 1990 ; Vol. 21, No. 8. pp. 805-810.
@article{d8333c274a3b4f1e839f7087592e3277,
title = "Nasopharyngeal carcinoma in children-A retrospective review and demonstration of epstein-barr viral genomes in tumor cell cytoplasm: A report of the pediatric oncology group",
abstract = "Twenty-seven nasopharyngeal carcinomas were entered in the Pediatric Oncology Group Rare Tumor Registry from 1973 to 1988 (15 males, 12 females; 10 white, 15 black, two unknown; ages 8 to 17 years). Eight tumors were non-keratinizing carcinomas (World Health Organization 2) and 19 were undifferentiated (World Health Organization 3). The overall 3-year survival rate was 70{\%} (SE 11{\%}). Nine children developed distant metastases and two were salvaged. We found that localized tumor (P = .02) and black race (P = .05) were associated with a better outcome. In situ hybridization using a biotinylated probe demonstrated Epstein-Barr virus DNA in the cytoplasm of the neoplastic epithelial cells in nine of 11 tumors examined, firmly establishing the presence of Epstein-Barr virus within the malignant cells of nasopharyngeal carcinomas of both World Health Organization 2 and World Health Organization 3 histology, rather than in the surrounding lymphocytes.",
keywords = "children, EBV-DNA, histochemical in situ hybridization, nasopharyngeal carcinoma",
author = "Hawkins, {Edith P.} and Krischer, {Jeffrey P.} and Smith, {Billie E.} and Hawkins, {Hal K.} and Finegold, {Milton J.}",
year = "1990",
doi = "10.1016/0046-8177(90)90049-B",
language = "English (US)",
volume = "21",
pages = "805--810",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "8",

}

TY - JOUR

T1 - Nasopharyngeal carcinoma in children-A retrospective review and demonstration of epstein-barr viral genomes in tumor cell cytoplasm

T2 - A report of the pediatric oncology group

AU - Hawkins, Edith P.

AU - Krischer, Jeffrey P.

AU - Smith, Billie E.

AU - Hawkins, Hal K.

AU - Finegold, Milton J.

PY - 1990

Y1 - 1990

N2 - Twenty-seven nasopharyngeal carcinomas were entered in the Pediatric Oncology Group Rare Tumor Registry from 1973 to 1988 (15 males, 12 females; 10 white, 15 black, two unknown; ages 8 to 17 years). Eight tumors were non-keratinizing carcinomas (World Health Organization 2) and 19 were undifferentiated (World Health Organization 3). The overall 3-year survival rate was 70% (SE 11%). Nine children developed distant metastases and two were salvaged. We found that localized tumor (P = .02) and black race (P = .05) were associated with a better outcome. In situ hybridization using a biotinylated probe demonstrated Epstein-Barr virus DNA in the cytoplasm of the neoplastic epithelial cells in nine of 11 tumors examined, firmly establishing the presence of Epstein-Barr virus within the malignant cells of nasopharyngeal carcinomas of both World Health Organization 2 and World Health Organization 3 histology, rather than in the surrounding lymphocytes.

AB - Twenty-seven nasopharyngeal carcinomas were entered in the Pediatric Oncology Group Rare Tumor Registry from 1973 to 1988 (15 males, 12 females; 10 white, 15 black, two unknown; ages 8 to 17 years). Eight tumors were non-keratinizing carcinomas (World Health Organization 2) and 19 were undifferentiated (World Health Organization 3). The overall 3-year survival rate was 70% (SE 11%). Nine children developed distant metastases and two were salvaged. We found that localized tumor (P = .02) and black race (P = .05) were associated with a better outcome. In situ hybridization using a biotinylated probe demonstrated Epstein-Barr virus DNA in the cytoplasm of the neoplastic epithelial cells in nine of 11 tumors examined, firmly establishing the presence of Epstein-Barr virus within the malignant cells of nasopharyngeal carcinomas of both World Health Organization 2 and World Health Organization 3 histology, rather than in the surrounding lymphocytes.

KW - children

KW - EBV-DNA

KW - histochemical in situ hybridization

KW - nasopharyngeal carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0025164378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025164378&partnerID=8YFLogxK

U2 - 10.1016/0046-8177(90)90049-B

DO - 10.1016/0046-8177(90)90049-B

M3 - Article

C2 - 2167287

AN - SCOPUS:0025164378

VL - 21

SP - 805

EP - 810

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 8

ER -