Naturally Occurring Tyzzer's Disease in a Calf

T. Ikegami, K. Shirota, Y. Une, Y. Nomura, Y. Wada, K. Goto, A. Takakura, T. Itoh, K. Fujiwara

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Naturally occurring Clostridium piliforme infection (Tyzzer's disease) was found in a calf. Light microscopic examination revealed multifocal coagulative necrosis in the liver, catarrhal gastroenteritis, tracheitis and pneumonia, and thymic atrophy. Warthin-Starry staining clearly showed large filamentous bacilli in bundles or criss-cross patterns within the hepatocytes and epithelium and smooth muscle cells of the ileum and cecum. Immunohistochemistry using anti-C. piliforme RT and MSK strain antisera showed positive reaction against the bacilli. Electron microscopic examination revealed bacilli within the hepatocytes that demonstrated a characteristic vegetative form, with peritrichous flagella, and spores. The polymerase chain reaction (PCR) study using the paraffin-embedded liver sections, the 196-bp DNA fragment specific to 16S ribosomal RNA of C. piliforme was amplified. The characteristics of these bacilli are consistent with those of C. piliforme. The PCR technique using paraffin-embedded sections should be useful for confirming C. piliforme infection in spontaneous cases.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalVeterinary Pathology
Issue number3
StatePublished - May 1999
Externally publishedYes


  • Calves
  • Clostridium piliforme
  • Multifocal hepatic necrosis
  • PCR
  • Tyzzer's disease

ASJC Scopus subject areas

  • General Veterinary


Dive into the research topics of 'Naturally Occurring Tyzzer's Disease in a Calf'. Together they form a unique fingerprint.

Cite this