Naturally Occurring Tyzzer's Disease in a Calf

Tetsuro Ikegami, K. Shirota, Y. Une, Y. Nomura, Y. Wada, K. Goto, A. Takakura, T. Itoh, K. Fujiwara

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Naturally occurring Clostridium piliforme infection (Tyzzer's disease) was found in a calf. Light microscopic examination revealed multifocal coagulative necrosis in the liver, catarrhal gastroenteritis, tracheitis and pneumonia, and thymic atrophy. Warthin-Starry staining clearly showed large filamentous bacilli in bundles or criss-cross patterns within the hepatocytes and epithelium and smooth muscle cells of the ileum and cecum. Immunohistochemistry using anti-C. piliforme RT and MSK strain antisera showed positive reaction against the bacilli. Electron microscopic examination revealed bacilli within the hepatocytes that demonstrated a characteristic vegetative form, with peritrichous flagella, and spores. The polymerase chain reaction (PCR) study using the paraffin-embedded liver sections, the 196-bp DNA fragment specific to 16S ribosomal RNA of C. piliforme was amplified. The characteristics of these bacilli are consistent with those of C. piliforme. The PCR technique using paraffin-embedded sections should be useful for confirming C. piliforme infection in spontaneous cases.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalVeterinary Pathology
Volume36
Issue number3
StatePublished - May 1999
Externally publishedYes

Fingerprint

Clostridium piliforme
Bacillus
calves
Paraffin
Hepatocytes
Tracheitis
alkanes
hepatocytes
16S Ribosomal RNA
Clostridium Infections
Polymerase Chain Reaction
polymerase chain reaction
Flagella
Cecum
tracheitis
Liver
Gastroenteritis
Spores
Ileum
liver

Keywords

  • Calves
  • Clostridium piliforme
  • Multifocal hepatic necrosis
  • PCR
  • Tyzzer's disease

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ikegami, T., Shirota, K., Une, Y., Nomura, Y., Wada, Y., Goto, K., ... Fujiwara, K. (1999). Naturally Occurring Tyzzer's Disease in a Calf. Veterinary Pathology, 36(3), 253-255.

Naturally Occurring Tyzzer's Disease in a Calf. / Ikegami, Tetsuro; Shirota, K.; Une, Y.; Nomura, Y.; Wada, Y.; Goto, K.; Takakura, A.; Itoh, T.; Fujiwara, K.

In: Veterinary Pathology, Vol. 36, No. 3, 05.1999, p. 253-255.

Research output: Contribution to journalArticle

Ikegami, T, Shirota, K, Une, Y, Nomura, Y, Wada, Y, Goto, K, Takakura, A, Itoh, T & Fujiwara, K 1999, 'Naturally Occurring Tyzzer's Disease in a Calf', Veterinary Pathology, vol. 36, no. 3, pp. 253-255.
Ikegami T, Shirota K, Une Y, Nomura Y, Wada Y, Goto K et al. Naturally Occurring Tyzzer's Disease in a Calf. Veterinary Pathology. 1999 May;36(3):253-255.
Ikegami, Tetsuro ; Shirota, K. ; Une, Y. ; Nomura, Y. ; Wada, Y. ; Goto, K. ; Takakura, A. ; Itoh, T. ; Fujiwara, K. / Naturally Occurring Tyzzer's Disease in a Calf. In: Veterinary Pathology. 1999 ; Vol. 36, No. 3. pp. 253-255.
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