Abstract
Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5857-5862 |
| Number of pages | 6 |
| Journal | Journal of Virology |
| Volume | 79 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 2005 |
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ASJC Scopus subject areas
- Immunology
Cite this
Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells. / Von Messling, Veronika; Oezguen, Numan; Zheng, Qi; Vongpunsawad, Sompong; Braun, Werner; Cattaneo, Roberto.
In: Journal of Virology, Vol. 79, No. 9, 05.2005, p. 5857-5862.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells
AU - Von Messling, Veronika
AU - Oezguen, Numan
AU - Zheng, Qi
AU - Vongpunsawad, Sompong
AU - Braun, Werner
AU - Cattaneo, Roberto
PY - 2005/5
Y1 - 2005/5
N2 - Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
AB - Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
UR - http://www.scopus.com/inward/record.url?scp=17444379724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444379724&partnerID=8YFLogxK
U2 - 10.1128/JVI.79.9.5857-5862.2005
DO - 10.1128/JVI.79.9.5857-5862.2005
M3 - Article
C2 - 15827201
AN - SCOPUS:17444379724
VL - 79
SP - 5857
EP - 5862
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 9
ER -