Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells

Veronika Von Messling, Numan Oezguen, Qi Zheng, Sompong Vongpunsawad, Werner Braun, Roberto Cattaneo

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.

Original languageEnglish (US)
Pages (from-to)5857-5862
Number of pages6
JournalJournal of virology
Volume79
Issue number9
DOIs
StatePublished - May 1 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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