Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells

Veronika Von Messling, Numan Oezguen, Qi Zheng, Sompong Vongpunsawad, Werner Braun, Roberto Cattaneo

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.

Original languageEnglish (US)
Pages (from-to)5857-5862
Number of pages6
JournalJournal of Virology
Volume79
Issue number9
DOIs
StatePublished - May 2005

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Canine Distemper Virus
Virus Internalization
Canine distemper virus
Hemagglutinins
hemagglutinins
mononuclear leukocytes
Blood Cells
Morbillivirus
Measles virus
mutation
Ferrets
Mutation
Membrane Fusion
Structural Models
ferrets
Canidae
epithelial cells
pathogenicity
Epithelial Cells
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells. / Von Messling, Veronika; Oezguen, Numan; Zheng, Qi; Vongpunsawad, Sompong; Braun, Werner; Cattaneo, Roberto.

In: Journal of Virology, Vol. 79, No. 9, 05.2005, p. 5857-5862.

Research output: Contribution to journalArticle

Von Messling, Veronika ; Oezguen, Numan ; Zheng, Qi ; Vongpunsawad, Sompong ; Braun, Werner ; Cattaneo, Roberto. / Nearby clusters of hemagglutinin residues sustain SLAM-dependent canine distemper virus entry in peripheral blood mononuclear cells. In: Journal of Virology. 2005 ; Vol. 79, No. 9. pp. 5857-5862.
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