Negevirus: A proposed new taxon of insect-specific viruses with wide geographic distribution

Nikos Vasilakis, Naomi L. Forrester, Gustavo Palacios, Farooq Nasar, Nazir Savji, Shannan Rossi, Hilda Guzman, Thomas Wood, Vsevolod Popov, Rodion Gorchakov, Ana Vázquez González, Andrew D. Haddow, Douglas M. Watts, Amelia P A Travassos da Rosa, Scott Weaver, W. Ian Lipkin, Robert B. Tesha

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Six novel insect-specific viruses, isolated from mosquitoes and phlebotomine sand flies collected in Brazil, Peru, the United States, Ivory Coast, Israel, and Indonesia, are described. Their genomes consist of single-stranded, positive-sense RNAs with poly(A) tails. By electron microscopy, the virions appear as spherical particles with diameters of ~45 to 55 nm. Based on their genome organization and phylogenetic relationship, the six viruses, designated Negev, Ngewotan, Piura, Loreto, Dezidougou, and Santana, appear to form a new taxon, tentatively designated Negevirus. Their closest but still distant relatives are citrus leposis virus C (CiLV-C) and viruses in the genus Cilevirus, which are mite-transmitted plant viruses. The negeviruses replicate rapidly and to high titer (up to 1010 PFU/ml) in mosquito cells, producing extensive cytopathic effect and plaques, but they do not appear to replicate in mammalian cells or mice. A discussion follows on their possible biological significance and effect on mosquito vector competence for arboviruses.

Original languageEnglish (US)
Pages (from-to)2475-2488
Number of pages14
JournalJournal of Virology
Volume87
Issue number5
DOIs
StatePublished - Mar 2013

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Insect Viruses
new taxa
geographical distribution
Viruses
Culicidae
viruses
insects
Phlebotominae
Cilevirus
Genome
Cote d'Ivoire
Arboviruses
Plant Viruses
Psychodidae
Peru
Indonesia
Citrus
Mites
Israel
vector competence

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Negevirus : A proposed new taxon of insect-specific viruses with wide geographic distribution. / Vasilakis, Nikos; Forrester, Naomi L.; Palacios, Gustavo; Nasar, Farooq; Savji, Nazir; Rossi, Shannan; Guzman, Hilda; Wood, Thomas; Popov, Vsevolod; Gorchakov, Rodion; González, Ana Vázquez; Haddow, Andrew D.; Watts, Douglas M.; da Rosa, Amelia P A Travassos; Weaver, Scott; Lipkin, W. Ian; Tesha, Robert B.

In: Journal of Virology, Vol. 87, No. 5, 03.2013, p. 2475-2488.

Research output: Contribution to journalArticle

Vasilakis, N, Forrester, NL, Palacios, G, Nasar, F, Savji, N, Rossi, S, Guzman, H, Wood, T, Popov, V, Gorchakov, R, González, AV, Haddow, AD, Watts, DM, da Rosa, APAT, Weaver, S, Lipkin, WI & Tesha, RB 2013, 'Negevirus: A proposed new taxon of insect-specific viruses with wide geographic distribution', Journal of Virology, vol. 87, no. 5, pp. 2475-2488. https://doi.org/10.1128/JVI.00776-12
Vasilakis, Nikos ; Forrester, Naomi L. ; Palacios, Gustavo ; Nasar, Farooq ; Savji, Nazir ; Rossi, Shannan ; Guzman, Hilda ; Wood, Thomas ; Popov, Vsevolod ; Gorchakov, Rodion ; González, Ana Vázquez ; Haddow, Andrew D. ; Watts, Douglas M. ; da Rosa, Amelia P A Travassos ; Weaver, Scott ; Lipkin, W. Ian ; Tesha, Robert B. / Negevirus : A proposed new taxon of insect-specific viruses with wide geographic distribution. In: Journal of Virology. 2013 ; Vol. 87, No. 5. pp. 2475-2488.
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