NEIL2-initiated, APE-independent repair of oxidized bases in DNA

Evidence for a repair complex in human cells

Aditi Das, Lee Wiederhold, John B. Leppard, Padmini Kedar, Rajendra Prasad, Huxian Wang, Istvan Boldogh, Feridoun Karimi-Busheri, Michael Weinfeld, Alan E. Tomkinson, Samuel H. Wilson, Sankar Mitra, Tapas Hazra

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

DNA glycosylases/AP lyases initiate repair of oxidized bases in the genomes of all organisms by excising these lesions and then cleaving the DNA strand at the resulting abasic (AP) sites and generate 3′ phospho α,β-unsaturated aldehyde (3′ PUA) or 3′ phosphate (3′ P) terminus. In Escherichia coli, the AP-endonucleases (APEs) hydrolyze both 3′ blocking groups (3′ PUA and 3′ P) to generate the 3′-OH termini needed for repair synthesis. In mammalian cells, the previously characterized DNA glycosylases, NTH1 and OGG1, produce 3′ PUA, which is removed by the only AP-endonuclease, APE1. However, APE1 is barely active in removing 3′ phosphate generated by the recently discovered mammalian DNA glycosylases NEIL1 and NEIL2. We showed earlier that the 3′ phosphate generated by NEIL1 is efficiently removed by polynucleotide kinase (PNK) and not APE1. Here we show that the NEIL2-initiated repair of 5-hydroxyuracil (5-OHU) similarly requires PNK. We have also observed stable interaction between NEIL2 and other BER proteins DNA polymerase β (Pol β), DNA ligase IIIα (Lig IIIα) and XRCC1. In spite of their limited sequence homology, NEIL1 and NEIL2 interact with the same domains of Pol β and Lig IIIα. Surprisingly, while the catalytically dispensable C-terminal region of NEIL1 is the common interacting domain, the essential N-terminal segment of NEIL2 is involved in analogous interaction. The BER proteins including NEIL2, PNK, Pol β, Lig IIIα and XRCC1 (but not APE1) could be isolated as a complex from human cells, competent for repair of 5-OHU in plasmid DNA.

Original languageEnglish (US)
Pages (from-to)1439-1448
Number of pages10
JournalDNA Repair
Volume5
Issue number12
DOIs
StatePublished - Dec 9 2006

Fingerprint

Polynucleotide 5'-Hydroxyl-Kinase
DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA Glycosylases
Aldehydes
Repair
Phosphates
Cells
DNA
DNA-Directed DNA Polymerase
Sequence Homology
DNA Ligases
Proteins
Plasmids
Genome
Escherichia coli
Genes

Keywords

  • Base excision repair
  • DNA glycosylase
  • NEIL2
  • Oxidative DNA damage
  • PNK-dependent repair

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

NEIL2-initiated, APE-independent repair of oxidized bases in DNA : Evidence for a repair complex in human cells. / Das, Aditi; Wiederhold, Lee; Leppard, John B.; Kedar, Padmini; Prasad, Rajendra; Wang, Huxian; Boldogh, Istvan; Karimi-Busheri, Feridoun; Weinfeld, Michael; Tomkinson, Alan E.; Wilson, Samuel H.; Mitra, Sankar; Hazra, Tapas.

In: DNA Repair, Vol. 5, No. 12, 09.12.2006, p. 1439-1448.

Research output: Contribution to journalArticle

Das, A, Wiederhold, L, Leppard, JB, Kedar, P, Prasad, R, Wang, H, Boldogh, I, Karimi-Busheri, F, Weinfeld, M, Tomkinson, AE, Wilson, SH, Mitra, S & Hazra, T 2006, 'NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells', DNA Repair, vol. 5, no. 12, pp. 1439-1448. https://doi.org/10.1016/j.dnarep.2006.07.003
Das, Aditi ; Wiederhold, Lee ; Leppard, John B. ; Kedar, Padmini ; Prasad, Rajendra ; Wang, Huxian ; Boldogh, Istvan ; Karimi-Busheri, Feridoun ; Weinfeld, Michael ; Tomkinson, Alan E. ; Wilson, Samuel H. ; Mitra, Sankar ; Hazra, Tapas. / NEIL2-initiated, APE-independent repair of oxidized bases in DNA : Evidence for a repair complex in human cells. In: DNA Repair. 2006 ; Vol. 5, No. 12. pp. 1439-1448.
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AU - Kedar, Padmini

AU - Prasad, Rajendra

AU - Wang, Huxian

AU - Boldogh, Istvan

AU - Karimi-Busheri, Feridoun

AU - Weinfeld, Michael

AU - Tomkinson, Alan E.

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