NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells

Altaf H. Sarker, Arpita Chatterjee, Monique Williams, Sabrina Lin, Christopher Havel, Peyton Jacob, Istvan Boldogh, Tapas Hazra, Prudence Talbot, Bo Hang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Secondhand smoke (SHS) is a confirmed lung carcinogen that introduces thousands of toxic chemicals into the lungs. SHS contains chemicals that have been implicated in causing oxidative DNA damage in the airway epithelium. Although DNA repair is considered a key defensive mechanism against various environmental attacks, such as cigarette smoking, the associations of individual repair enzymes with susceptibility to lung cancer are largely unknown. This study investigated the role of NEIL2, a DNA glycosylase excising oxidative base lesions, in human lung cells treated with sidestream smoke (SSS), the main component of SHS. To do so, we generated NEIL2 knockdown cells using siRNA-technology and exposed them to SSS-laden medium. Representative SSS chemical compounds in the medium were analyzed by mass spectrometry. An increased production of reactive oxygen species (ROS) in SSS-exposed cells was detected through the fluorescent detection and the induction of HIF-1α. The long amplicon-quantitative PCR (LA-QPCR) assay detected significant dose-dependent increases of oxidative DNA damage in the HPRT gene of cultured human pulmonary fibroblasts (hPF) and BEAS-2B epithelial cells exposed to SSS for 24 h. These data suggest that SSS exposure increased oxidative stress, which could contribute to SSS-mediated toxicity. siRNA knockdown of NEIL2 in hPF and HEK 293 cells exposed to SSS for 24 h resulted in significantly more oxidative DNA damage in HPRT and POLB than in cells with control siRNA. Taken together, our data strongly suggest that decreased repair of oxidative DNA base lesions due to an impaired NEIL2 expression in non-smokers exposed to SSS would lead to accumulation of mutations in genomic DNA of lung cells over time, thus contributing to the onset of SSS-induced lung cancer.

Original languageEnglish (US)
Article numbere90261
JournalPLoS One
Volume9
Issue number3
DOIs
StatePublished - Mar 3 2014

Fingerprint

smoke
Smoke
DNA damage
DNA Damage
Cells
DNA
Lung
Tobacco Smoke Pollution
cells
lungs
Small Interfering RNA
Hypoxanthine Phosphoribosyltransferase
small interfering RNA
DNA Repair
Repair
Lung Neoplasms
Fibroblasts
lung neoplasms
lesions (animal)
DNA Glycosylases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sarker, A. H., Chatterjee, A., Williams, M., Lin, S., Havel, C., Jacob, P., ... Hang, B. (2014). NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells. PLoS One, 9(3), [e90261]. https://doi.org/10.1371/journal.pone.0090261

NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells. / Sarker, Altaf H.; Chatterjee, Arpita; Williams, Monique; Lin, Sabrina; Havel, Christopher; Jacob, Peyton; Boldogh, Istvan; Hazra, Tapas; Talbot, Prudence; Hang, Bo.

In: PLoS One, Vol. 9, No. 3, e90261, 03.03.2014.

Research output: Contribution to journalArticle

Sarker, AH, Chatterjee, A, Williams, M, Lin, S, Havel, C, Jacob, P, Boldogh, I, Hazra, T, Talbot, P & Hang, B 2014, 'NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells', PLoS One, vol. 9, no. 3, e90261. https://doi.org/10.1371/journal.pone.0090261
Sarker AH, Chatterjee A, Williams M, Lin S, Havel C, Jacob P et al. NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells. PLoS One. 2014 Mar 3;9(3). e90261. https://doi.org/10.1371/journal.pone.0090261
Sarker, Altaf H. ; Chatterjee, Arpita ; Williams, Monique ; Lin, Sabrina ; Havel, Christopher ; Jacob, Peyton ; Boldogh, Istvan ; Hazra, Tapas ; Talbot, Prudence ; Hang, Bo. / NEIL2 Protects against Oxidative DNA damage induced by sidestream smoke in human cells. In: PLoS One. 2014 ; Vol. 9, No. 3.
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