Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats

Jeffrey R. Kirsch, Anish Bhardwaj, Lee J. Martin, Daniel F. Hanley, Richard J. Traystman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background and Purpose: We attempted to determine whether N(ω)-nitro- L-arginine methyl ester (L-NAME) would improve neurological outcome and whether L-arginine (L-ARG) would worsen neurological outcome after transient global ischemia. Methods: Halothane-anesthetized cats (n=6 for each group) were treated with intravenous saline, L-NAME (5 mg/kg or 10 mg/kg), or L- arginine (300 kg) 30 minutes before 10 minutes of ischemia (temporary ligation of the left subclavian and brachiocephalic arteries with hemorrhagic hypotension to 50 mm Hg). At 30 minutes of reperfusion, cats in the L-ARG group were administered an additional 300 mg/kg dose of intravenous L- arginine. Results: Time (mean±SE) to isoelectric electroencephalography was similar among groups (saline, 26±11 seconds; L-NAME-5, 15±4 seconds; L- NAME-10, 36±27 seconds; and L-ARG, 22±7 seconds). At 72 hours, reperfusion pathological injury was severe and neurological deficit score (mean, range) was similar among groups (saline, 38 [11 to 70]; L-NAME-5, 52 [40 to 73]; L- NAME-10, 47 [23 to 70]; and L-ARG, 40 [0 to 79]). Conclusions: Nitric oxide is not important in the mechanism of brain injury after global ischemia in cats.

Original languageEnglish (US)
Pages (from-to)2259-2265
Number of pages7
JournalStroke
Volume28
Issue number11
StatePublished - Nov 1997
Externally publishedYes

Fingerprint

NG-Nitroarginine Methyl Ester
Arginine
Cats
Ischemia
Subclavian Artery
Halothane
Reperfusion Injury
Hypotension
Brain Injuries
Reperfusion
Ligation
Electroencephalography
Nitric Oxide

Keywords

  • Cats
  • Cerebral ischemia
  • Global
  • Neuronal death
  • Neuroprotection
  • Nitric oxide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Kirsch, J. R., Bhardwaj, A., Martin, L. J., Hanley, D. F., & Traystman, R. J. (1997). Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats. Stroke, 28(11), 2259-2265.

Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats. / Kirsch, Jeffrey R.; Bhardwaj, Anish; Martin, Lee J.; Hanley, Daniel F.; Traystman, Richard J.

In: Stroke, Vol. 28, No. 11, 11.1997, p. 2259-2265.

Research output: Contribution to journalArticle

Kirsch, JR, Bhardwaj, A, Martin, LJ, Hanley, DF & Traystman, RJ 1997, 'Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats', Stroke, vol. 28, no. 11, pp. 2259-2265.
Kirsch JR, Bhardwaj A, Martin LJ, Hanley DF, Traystman RJ. Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats. Stroke. 1997 Nov;28(11):2259-2265.
Kirsch, Jeffrey R. ; Bhardwaj, Anish ; Martin, Lee J. ; Hanley, Daniel F. ; Traystman, Richard J. / Neither L-arginine nor L-NAME affects neurological outcome after global ischemia in cats. In: Stroke. 1997 ; Vol. 28, No. 11. pp. 2259-2265.
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