Neonatal sciatic nerve lesion triggers the sprouting of fibers in the contralateral ventral root of the rat

Kyungsoon Chung, Mae Ja Park, Kwangsup Sheen, Jin Chung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We explored the possibility that unilateral neurectomy of the sciatic nerve of the rat at the neonatal stage triggers sprouting of afferent fibers in the contralateral ventral root. 3 months after neonatal sciatic neurectomy, the numbers of both myelinated and unmyelinated fibers in the L5 and L3 ventral roots were counted on electron micrographic montages. Age-matched littermates were used as unoperated controls. To identify regenerating axons, electron microscopic immunohistochemistry was done on the ventral roots using antibody against growth-associated phosphoprotein (GAP-43). Neonatal sciatic neurectomy resulted in: (1) about a three-fold increase in the number of unmyelinated fibers in the contralateral L5 ventral root as compared with the unoperated control; (2) about a 25-fold increase in the number of unmyelinated fibers in the ipsilateral L5 ventral root as compared with the control; (3) ∼25% of the unmyelinated fibers in the contralateral L5 ventral root expressing GAP-43; and (4) no significant change in the number of unmyelinated fibers in the L3 ventral root of either side as compared with the control. The data suggest that a neonatal sciatic neurectomy of the rat triggers sprouting of unmyelinated afferent fibers in the ventral root of the contralateral as well as the ipsilateral side. The sprouting is restricted, however, to spinal segments with receive inputs from the sciatic nerve.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalBrain Research
Volume632
Issue number1-2
DOIs
StatePublished - Dec 31 1993

Fingerprint

Sciatic Neuropathy
Spinal Nerve Roots
GAP-43 Protein
Sciatic Nerve
Electrons
Phosphoproteins
Axons
Immunohistochemistry

Keywords

  • Dorsal root ganglion
  • GAP-43
  • Regeneration
  • Unmyelinated fiber

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neonatal sciatic nerve lesion triggers the sprouting of fibers in the contralateral ventral root of the rat. / Chung, Kyungsoon; Park, Mae Ja; Sheen, Kwangsup; Chung, Jin.

In: Brain Research, Vol. 632, No. 1-2, 31.12.1993, p. 80-85.

Research output: Contribution to journalArticle

Chung, Kyungsoon ; Park, Mae Ja ; Sheen, Kwangsup ; Chung, Jin. / Neonatal sciatic nerve lesion triggers the sprouting of fibers in the contralateral ventral root of the rat. In: Brain Research. 1993 ; Vol. 632, No. 1-2. pp. 80-85.
@article{91b68408467e4ecdafb9b0281ec34734,
title = "Neonatal sciatic nerve lesion triggers the sprouting of fibers in the contralateral ventral root of the rat",
abstract = "We explored the possibility that unilateral neurectomy of the sciatic nerve of the rat at the neonatal stage triggers sprouting of afferent fibers in the contralateral ventral root. 3 months after neonatal sciatic neurectomy, the numbers of both myelinated and unmyelinated fibers in the L5 and L3 ventral roots were counted on electron micrographic montages. Age-matched littermates were used as unoperated controls. To identify regenerating axons, electron microscopic immunohistochemistry was done on the ventral roots using antibody against growth-associated phosphoprotein (GAP-43). Neonatal sciatic neurectomy resulted in: (1) about a three-fold increase in the number of unmyelinated fibers in the contralateral L5 ventral root as compared with the unoperated control; (2) about a 25-fold increase in the number of unmyelinated fibers in the ipsilateral L5 ventral root as compared with the control; (3) ∼25{\%} of the unmyelinated fibers in the contralateral L5 ventral root expressing GAP-43; and (4) no significant change in the number of unmyelinated fibers in the L3 ventral root of either side as compared with the control. The data suggest that a neonatal sciatic neurectomy of the rat triggers sprouting of unmyelinated afferent fibers in the ventral root of the contralateral as well as the ipsilateral side. The sprouting is restricted, however, to spinal segments with receive inputs from the sciatic nerve.",
keywords = "Dorsal root ganglion, GAP-43, Regeneration, Unmyelinated fiber",
author = "Kyungsoon Chung and Park, {Mae Ja} and Kwangsup Sheen and Jin Chung",
year = "1993",
month = "12",
day = "31",
doi = "10.1016/0006-8993(93)91141-E",
language = "English (US)",
volume = "632",
pages = "80--85",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Neonatal sciatic nerve lesion triggers the sprouting of fibers in the contralateral ventral root of the rat

AU - Chung, Kyungsoon

AU - Park, Mae Ja

AU - Sheen, Kwangsup

AU - Chung, Jin

PY - 1993/12/31

Y1 - 1993/12/31

N2 - We explored the possibility that unilateral neurectomy of the sciatic nerve of the rat at the neonatal stage triggers sprouting of afferent fibers in the contralateral ventral root. 3 months after neonatal sciatic neurectomy, the numbers of both myelinated and unmyelinated fibers in the L5 and L3 ventral roots were counted on electron micrographic montages. Age-matched littermates were used as unoperated controls. To identify regenerating axons, electron microscopic immunohistochemistry was done on the ventral roots using antibody against growth-associated phosphoprotein (GAP-43). Neonatal sciatic neurectomy resulted in: (1) about a three-fold increase in the number of unmyelinated fibers in the contralateral L5 ventral root as compared with the unoperated control; (2) about a 25-fold increase in the number of unmyelinated fibers in the ipsilateral L5 ventral root as compared with the control; (3) ∼25% of the unmyelinated fibers in the contralateral L5 ventral root expressing GAP-43; and (4) no significant change in the number of unmyelinated fibers in the L3 ventral root of either side as compared with the control. The data suggest that a neonatal sciatic neurectomy of the rat triggers sprouting of unmyelinated afferent fibers in the ventral root of the contralateral as well as the ipsilateral side. The sprouting is restricted, however, to spinal segments with receive inputs from the sciatic nerve.

AB - We explored the possibility that unilateral neurectomy of the sciatic nerve of the rat at the neonatal stage triggers sprouting of afferent fibers in the contralateral ventral root. 3 months after neonatal sciatic neurectomy, the numbers of both myelinated and unmyelinated fibers in the L5 and L3 ventral roots were counted on electron micrographic montages. Age-matched littermates were used as unoperated controls. To identify regenerating axons, electron microscopic immunohistochemistry was done on the ventral roots using antibody against growth-associated phosphoprotein (GAP-43). Neonatal sciatic neurectomy resulted in: (1) about a three-fold increase in the number of unmyelinated fibers in the contralateral L5 ventral root as compared with the unoperated control; (2) about a 25-fold increase in the number of unmyelinated fibers in the ipsilateral L5 ventral root as compared with the control; (3) ∼25% of the unmyelinated fibers in the contralateral L5 ventral root expressing GAP-43; and (4) no significant change in the number of unmyelinated fibers in the L3 ventral root of either side as compared with the control. The data suggest that a neonatal sciatic neurectomy of the rat triggers sprouting of unmyelinated afferent fibers in the ventral root of the contralateral as well as the ipsilateral side. The sprouting is restricted, however, to spinal segments with receive inputs from the sciatic nerve.

KW - Dorsal root ganglion

KW - GAP-43

KW - Regeneration

KW - Unmyelinated fiber

UR - http://www.scopus.com/inward/record.url?scp=0027749481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027749481&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(93)91141-E

DO - 10.1016/0006-8993(93)91141-E

M3 - Article

C2 - 8149247

AN - SCOPUS:0027749481

VL - 632

SP - 80

EP - 85

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -