Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.

J. L. Redpath; C. Sun; M. Colman; E. J. Stanbridge

doi:10.2307/3577013

Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.

J. L. Redpath, C. Sun, M. Colman, E. J. Stanbridge

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.

Original language	English (US)
Pages (from-to)	468-472
Number of pages	5
Journal	Radiation research
Volume	110
Issue number	3
DOIs	https://doi.org/10.2307/3577013
State	Published - Jun 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Radiation
Radiology Nuclear Medicine and imaging

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title = "Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.",

abstract = "Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.",

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T1 - Neoplastic transformation of human hybrid cells by gamma radiation

T2 - a quantitative assay.

AU - Redpath, J. L.

AU - Sun, C.

AU - Colman, M.

AU - Stanbridge, E. J.

PY - 1987/6

Y1 - 1987/6

N2 - Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.

AB - Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.

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Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.

Abstract

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