Neoplastic transformation of human hybrid cells by gamma radiation

a quantitative assay.

J. L. Redpath, C. Sun, M. Colman, E. J. Stanbridge

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.

Original languageEnglish (US)
Pages (from-to)468-472
Number of pages5
JournalRadiation Research
Volume110
Issue number3
StatePublished - Jun 1987
Externally publishedYes

Fingerprint

Hybrid Cells
Gamma Rays
fibroblasts
cultured cells
ionizing radiation
gamma radiation
gamma rays
Ionizing Radiation
skin (animal)
dosage
assays
refractories
Fibroblasts
cells
cell lines
Cell Line
Skin
dose response

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Redpath, J. L., Sun, C., Colman, M., & Stanbridge, E. J. (1987). Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay. Radiation Research, 110(3), 468-472.

Neoplastic transformation of human hybrid cells by gamma radiation : a quantitative assay. / Redpath, J. L.; Sun, C.; Colman, M.; Stanbridge, E. J.

In: Radiation Research, Vol. 110, No. 3, 06.1987, p. 468-472.

Research output: Contribution to journalArticle

Redpath, JL, Sun, C, Colman, M & Stanbridge, EJ 1987, 'Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.', Radiation Research, vol. 110, no. 3, pp. 468-472.
Redpath, J. L. ; Sun, C. ; Colman, M. ; Stanbridge, E. J. / Neoplastic transformation of human hybrid cells by gamma radiation : a quantitative assay. In: Radiation Research. 1987 ; Vol. 110, No. 3. pp. 468-472.
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