Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay.

J. L. Redpath, C. Sun, M. Colman, E. J. Stanbridge

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Abstract

Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.

Original languageEnglish (US)
Pages (from-to)468-472
Number of pages5
JournalRadiation Research
Volume110
Issue number3
StatePublished - Jun 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Redpath, J. L., Sun, C., Colman, M., & Stanbridge, E. J. (1987). Neoplastic transformation of human hybrid cells by gamma radiation: a quantitative assay. Radiation Research, 110(3), 468-472.