Nerve growth factor as a mitogen for a pancreatic carcinoid cell line

R. J. Bold, J. Ishizuka, S. Rajaraman, J. R. Perez-Polo, Courtney Townsend, J. C. Thompson

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Carcinoid tumors are a group of neuroendocrine neoplasms distributed widely throughout the body but most commonly occurring in the gut. These tumors retain many characteristics of their neural crest origin, including secretion of neuroactive peptides and responsiveness to neurotrophic substances. Nerve growth factor (NGF), a neurotrophic protein involved in maintenance and differentiation of peripheral sympathetic and sensory neurons, regulates growth of several neural tumor cells by inducing a differentiated phenotype and subsequent inhibition of cell growth rate. We examined the actions of NGF in a functioning human pancreatic carcinoid cell line (termed BON). NGF has no effect on the cytoarchitecture or constitutive secretion of bioamines in this carcinoid cell line. NGF, however, stimulates the in vitro cellular proliferation of BON cells. BON cells possess mRNA for the NGF receptors (p75(LNGFR) and p140(trkA)) and membrane-associated tyrosine kinase activity is increased in response to NGF. Both the mitogenic activity of NGF, as well as the receptor-linked tyrosine kinase activity, can be abrogated in BON cells by the trkA inhibitor K-252a and specific anti-NGF antibody. Our studies demonstrate that NGF is a mitogen for this carcinoid cell line without effect on cellular phenotype or cytoarchitecture. NGF may play a role in the development and progression of human carcinoid tumors.

    Original languageEnglish (US)
    Pages (from-to)2622-2628
    Number of pages7
    JournalJournal of Neurochemistry
    Volume64
    Issue number6
    StatePublished - 1995

    Fingerprint

    Carcinoid Tumor
    Nerve Growth Factor
    Mitogens
    Cells
    Cell Line
    Tumors
    Nerve Growth Factor Receptor
    Protein-Tyrosine Kinases
    trkA Receptor
    Phenotype
    Neoplasms
    Nerve Growth Factors
    Cell growth
    Neural Crest
    Receptor Protein-Tyrosine Kinases
    Human Development
    Sensory Receptor Cells
    Growth
    Neurons
    Membranes

    Keywords

    • Carcinoid
    • Mitogen
    • Nerve growth factor

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Bold, R. J., Ishizuka, J., Rajaraman, S., Perez-Polo, J. R., Townsend, C., & Thompson, J. C. (1995). Nerve growth factor as a mitogen for a pancreatic carcinoid cell line. Journal of Neurochemistry, 64(6), 2622-2628.

    Nerve growth factor as a mitogen for a pancreatic carcinoid cell line. / Bold, R. J.; Ishizuka, J.; Rajaraman, S.; Perez-Polo, J. R.; Townsend, Courtney; Thompson, J. C.

    In: Journal of Neurochemistry, Vol. 64, No. 6, 1995, p. 2622-2628.

    Research output: Contribution to journalArticle

    Bold, RJ, Ishizuka, J, Rajaraman, S, Perez-Polo, JR, Townsend, C & Thompson, JC 1995, 'Nerve growth factor as a mitogen for a pancreatic carcinoid cell line', Journal of Neurochemistry, vol. 64, no. 6, pp. 2622-2628.
    Bold RJ, Ishizuka J, Rajaraman S, Perez-Polo JR, Townsend C, Thompson JC. Nerve growth factor as a mitogen for a pancreatic carcinoid cell line. Journal of Neurochemistry. 1995;64(6):2622-2628.
    Bold, R. J. ; Ishizuka, J. ; Rajaraman, S. ; Perez-Polo, J. R. ; Townsend, Courtney ; Thompson, J. C. / Nerve growth factor as a mitogen for a pancreatic carcinoid cell line. In: Journal of Neurochemistry. 1995 ; Vol. 64, No. 6. pp. 2622-2628.
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