Nerve growth factor induces neurite outgrowth in a clone derived from an NGF-insensitive human neuroblastoma cell line

C. Patrick Reynolds, J. Regino Perez-Poto

    Research output: Contribution to journalArticle

    10 Scopus citations

    Abstract

    Nerve growth factor (NGF) plays a trophic and tropic role in the development of vertebrate sympathetic and sensory ganglia; however, the precise nature of the NGF effect(s) is not understood. Study of NGF-responsive human neuroblastoma cell lines allows characterization of NGF-induced neunte outgrowth in cells not dependent on NGF for survival. The human neuroblastoma line SK-N-SH did not significantly extend neuntes in response to NGF, but did show an increase in cell number. By contrast, a clone of the line, SH-IN, extended neuntes in response to NGF or dibutyryl cyclic AMP, and showed inhibition of cell proliferation. Thus, cells capable of morphological differentiation in response to NGF can be cloned from neuroblastoma cell lines in which the majority of the cells fail to extend neurites even in the presence of NGF.

    Original languageEnglish (US)
    Pages (from-to)125-127,129-132
    JournalInternational Journal of Developmental Neuroscience
    Volume7
    Issue number2
    DOIs
    StatePublished - 1989

    Keywords

    • Growth factor
    • NGF
    • Neurite
    • Neuroblastoma
    • Tissue culture

    ASJC Scopus subject areas

    • Developmental Neuroscience
    • Developmental Biology

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