Nerve growth factor rapidly suppresses basal, NMDA-evoked, and AMPA- evoked nitric oxide synthase activity in rat hippocampus in vivo

H. H D Lam, Anish Bhardwaj, M. T. O'Connell, D. F. Hanley, R. J. Traystman, M. V. Sofroniew

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In adult forebrain, nerve growth factor (NGF) influences neuronal maintenance and axon sprouting and is neuroprotective in several injury models through mechanisms that are incompletely understood. Most NGF signaling is thought to occur after internalization and retrograde transport of trkA receptor and be mediated through the nucleus. However, NGF expression in hippocampus is rapidly and sensitively regulated by synaptic activity, suggesting that NGF exerts local effects more dynamically than possible through signaling requiring retrograde transport to distant afferent neurons. Interactions have been reported between NGF and nitric oxide (NO). Because NO affects both neural plasticity and degeneration, and trk receptors can mediate signaling within minutes, we hypothesized that NGF might rapidly modulate NO production. Using in vivo microdialysis we measured conversion of L-[14C]arginine to L-[14C]citrulline as an accurate reflection of NO synthase (NOS) activity in adult rat hippocampus. NGF significantly reduced NOS activity to 61% of basal levels within 20 min of onset of delivery and maintained NOS activity at less than 50% of baseline throughout 3 hr of delivery. This effect did not occur with control protein (cytochrome c) and was not mediated by an effect of NGF on glutamate levels. In addition, simultaneous delivery of NGF prevented significant increases in NOS activity triggered by the glutamate receptor agonists N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). Rapid suppression by NGF of basal and glutamate-stimulated NOS activity may regulate neuromodulatory functions of NO or protect neurons from NO toxicity and suggests a novel mechanism for rapidly mediating functions of NGF and other neurotrophins.

Original languageEnglish (US)
Pages (from-to)10926-10931
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number18
DOIs
StatePublished - Sep 1 1998
Externally publishedYes

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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Nerve Growth Factor
N-Methylaspartate
Nitric Oxide Synthase
Hippocampus
Nitric Oxide
Glutamic Acid
trkA Receptor
Excitatory Amino Acid Agonists
Afferent Neurons
Citrulline
Neuronal Plasticity
Microdialysis
Nerve Growth Factors
Glutamate Receptors
Prosencephalon
Cytochromes c
Axons
Arginine
Maintenance

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nerve growth factor rapidly suppresses basal, NMDA-evoked, and AMPA- evoked nitric oxide synthase activity in rat hippocampus in vivo. / Lam, H. H D; Bhardwaj, Anish; O'Connell, M. T.; Hanley, D. F.; Traystman, R. J.; Sofroniew, M. V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 18, 01.09.1998, p. 10926-10931.

Research output: Contribution to journalArticle

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