Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons

John Winston, Hiroki Toma, Mohan Shenoy, Pankaj Jay Pasricha

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalPain
Volume89
Issue number2-3
DOIs
StatePublished - 2001

Fingerprint

Capsaicin
Spinal Ganglia
Nerve Growth Factor
Neurons
Messenger RNA
Nerve Growth Factor Receptor
Calcitonin Gene-Related Peptide
TRPV Cation Channels
Neuropeptides
vanilloid receptor subtype 1

Keywords

  • Calcitonin gene related peptide release
  • Dorsal root ganglion
  • Nerve growth factor
  • Vanilloid receptor

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons. / Winston, John; Toma, Hiroki; Shenoy, Mohan; Pasricha, Pankaj Jay.

In: Pain, Vol. 89, No. 2-3, 2001, p. 181-186.

Research output: Contribution to journalArticle

Winston, John ; Toma, Hiroki ; Shenoy, Mohan ; Pasricha, Pankaj Jay. / Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons. In: Pain. 2001 ; Vol. 89, No. 2-3. pp. 181-186.
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