Nested PCR with the PGMY09/11 and GP5 +/6 + primer sets improves detection of HPV DNA in cervical samples

Andrea L. Fuessel Haws, Qin He, Peter L. Rady, Lifang Zhang, James Grady, Thomas K. Hughes, Kendra Stisser, Rolf Konig, Stephen K. Tyring

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Based on epidemiological and research evidence, HPV has a causal role in cervical carcinogenesis. Several HPV detection methods exist to date; the most commonly used method for detection of genital HPVs consists of nested PCR using the MY09/11 and GP5 +/6 + primer sets (MY/GP +). Recently, the PGMY09/11 primer set, a modified version of the MY09/11 primer set, was introduced for single PCR and was found to detect a wider range of HPV types. The next logical step was taken and the efficacy of nested PCR using the PGMY09/11 and GP5 +/6 + primer sets (PGMY/GP +) to detect HPV in cervical samples was evaluated. In this comparative study, nested PCR using the novel PGMY/GP + primer set combination was found to be more type sensitive than the nested PCR with the MY/GP + primer sets, detecting a wider range of HPV types, low copy HPVs, and better characterizing samples infected with multiple strains of HPV. Standardization and use of the PGMY/GP + PCR system could aid physicians in providing more efficient HPV screening and better treatment for patients.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalJournal of Virological Methods
Volume122
Issue number1
DOIs
StatePublished - Dec 1 2004

Keywords

  • Detection
  • GP
  • Human papillomavirus
  • PCR
  • PGMY

ASJC Scopus subject areas

  • Virology

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    Fuessel Haws, A. L., He, Q., Rady, P. L., Zhang, L., Grady, J., Hughes, T. K., Stisser, K., Konig, R., & Tyring, S. K. (2004). Nested PCR with the PGMY09/11 and GP5 +/6 + primer sets improves detection of HPV DNA in cervical samples. Journal of Virological Methods, 122(1), 87-93. https://doi.org/10.1016/j.jviromet.2004.08.007