Abstract
P2-fractions were isolated from rat brain, and used to study net taurine transport. The fractions were incubated in increasing concentrations of [3H]taurine and the intraterminal concentration measured by liquid scintillation and amino acid analysis. The membrane potential of the isolated fractions was estimated using86Rb+ as a marker for intracellular K+. Taurine was synthesized in the P2-fraction when incubated in taurine free medium. At external taurine concentrations below 370 μM a significant amount of the endogenous taurine was released to the incubation medium. Net taurine uptake into the P2-fraction was achieved at external taurine concentrations exceeding 370 μM. The taurine antagonist 6-aminomethyl-3-methyl-4H, 1, 2, 4-benzothiadiazine-1, 1-dioxide (TAG) competitively inhibited taurine and [3H]taurine transport into the P2-fraction. As the external concentration of taurine was increased, the accumulation of86Rb+ into the P2-fraction was facilitated. This indicated an increasing hyperpolarization of the neuronal membrane as taurine transport shifted from release towards uptake. TAG reduced the hyperpolarization that paralleled taurine accumulation, in a dose dependent manner. Our results indicate that relatively low transmembranal gradients of taurine may be maintained by an electrogenic taurine transporter having a large transport capacity. Such a transporter may well serve the needs of osmotic regulation, i.e. to transport large amounts of taurine in any direction across the neuronal membrane.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 347-352 |
| Number of pages | 6 |
| Journal | Neurochemical Research |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1994 |
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Keywords
- ion gradients
- membrane potential
- nerve terminals
- osmo-regulator
- taurine antagonist
- Taurine transport
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry
Cite this
Net taurine transport and its inhibition by a taurine antagonist. / Lewin, Lena; Rassin, David K.; Sellström, Åke.
In: Neurochemical Research, Vol. 19, No. 3, 03.1994, p. 347-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Net taurine transport and its inhibition by a taurine antagonist
AU - Lewin, Lena
AU - Rassin, David K.
AU - Sellström, Åke
PY - 1994/3
Y1 - 1994/3
N2 - P2-fractions were isolated from rat brain, and used to study net taurine transport. The fractions were incubated in increasing concentrations of [3H]taurine and the intraterminal concentration measured by liquid scintillation and amino acid analysis. The membrane potential of the isolated fractions was estimated using86Rb+ as a marker for intracellular K+. Taurine was synthesized in the P2-fraction when incubated in taurine free medium. At external taurine concentrations below 370 μM a significant amount of the endogenous taurine was released to the incubation medium. Net taurine uptake into the P2-fraction was achieved at external taurine concentrations exceeding 370 μM. The taurine antagonist 6-aminomethyl-3-methyl-4H, 1, 2, 4-benzothiadiazine-1, 1-dioxide (TAG) competitively inhibited taurine and [3H]taurine transport into the P2-fraction. As the external concentration of taurine was increased, the accumulation of86Rb+ into the P2-fraction was facilitated. This indicated an increasing hyperpolarization of the neuronal membrane as taurine transport shifted from release towards uptake. TAG reduced the hyperpolarization that paralleled taurine accumulation, in a dose dependent manner. Our results indicate that relatively low transmembranal gradients of taurine may be maintained by an electrogenic taurine transporter having a large transport capacity. Such a transporter may well serve the needs of osmotic regulation, i.e. to transport large amounts of taurine in any direction across the neuronal membrane.
AB - P2-fractions were isolated from rat brain, and used to study net taurine transport. The fractions were incubated in increasing concentrations of [3H]taurine and the intraterminal concentration measured by liquid scintillation and amino acid analysis. The membrane potential of the isolated fractions was estimated using86Rb+ as a marker for intracellular K+. Taurine was synthesized in the P2-fraction when incubated in taurine free medium. At external taurine concentrations below 370 μM a significant amount of the endogenous taurine was released to the incubation medium. Net taurine uptake into the P2-fraction was achieved at external taurine concentrations exceeding 370 μM. The taurine antagonist 6-aminomethyl-3-methyl-4H, 1, 2, 4-benzothiadiazine-1, 1-dioxide (TAG) competitively inhibited taurine and [3H]taurine transport into the P2-fraction. As the external concentration of taurine was increased, the accumulation of86Rb+ into the P2-fraction was facilitated. This indicated an increasing hyperpolarization of the neuronal membrane as taurine transport shifted from release towards uptake. TAG reduced the hyperpolarization that paralleled taurine accumulation, in a dose dependent manner. Our results indicate that relatively low transmembranal gradients of taurine may be maintained by an electrogenic taurine transporter having a large transport capacity. Such a transporter may well serve the needs of osmotic regulation, i.e. to transport large amounts of taurine in any direction across the neuronal membrane.
KW - ion gradients
KW - membrane potential
KW - nerve terminals
KW - osmo-regulator
KW - taurine antagonist
KW - Taurine transport
UR - http://www.scopus.com/inward/record.url?scp=0028031897&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028031897&partnerID=8YFLogxK
U2 - 10.1007/BF00971584
DO - 10.1007/BF00971584
M3 - Article
C2 - 8177375
AN - SCOPUS:0028031897
VL - 19
SP - 347
EP - 352
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 3
ER -