Neuroinflammation modulates distinct regional and temporal clinical responses in ALS mice

David R. Beers, Weihua Zhao, Bing Liao, Osamu Kano, Jinghong Wang, Ailing Huang, Stanley H. Appel, Jenny S. Henkel

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

An inflammatory response is a pathological hallmark of amyotrophic lateral sclerosis (ALS), a relentless and devastating degenerative disease of motoneurons. This response is not simply a late consequence of motoneuron degeneration, but actively contributes to the balance between neuroprotection and neurotoxicity; initially infiltrating lymphocytes and microglia slow disease progression, while later, they contribute to the acceleration of disease. Since motor weakness begins in the hindlimbs of ALS mice and only later involves the forelimbs, we determined whether differential protective versus injurious inflammatory responses in the cervical and lumbar spinal cords explained the temporally distinct clinical disease courses between the limbs of these mice. Densitometric evaluation of immunohistochemical sections and quantitative RT-PCR (qRT-PCR) demonstrated that CD68 and CD11c were differentially increased in their spinals cords. qRT-PCR revealed that protective and anti-inflammatory factors, including BDNF, GDNF, and IL-4, were increased in the cervical region compared with the lumbar region. In contrast, the toxic markers TNF-α, IL-1β and NOX2 were not different between ALS mice cervical and lumbar regions. T lymphocytes were observed infiltrating lumbar spinal cords of ALS mice prior to the cervical region; mRNA levels of the transcription factor gata-3 (Th2 response) were differentially elevated in the cervical cord of ALS mice whereas t-bet (Th1 response) was increased in the lumbar cord. These results reinforce the important balance between specific protective/injurious inflammatory immune responses in modulating clinical outcomes and suggest that the delayed forelimb motor weakness in ALS mice is partially explained by augmented protective responses in the cervical spinal cords.

Original languageEnglish (US)
Pages (from-to)1025-1035
Number of pages11
JournalBrain, Behavior, and Immunity
Volume25
Issue number5
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

Fingerprint

Amyotrophic Lateral Sclerosis
Spinal Cord
Lumbosacral Region
Forelimb
Motor Neurons
Transcription Factor 3
Glial Cell Line-Derived Neurotrophic Factor
Polymerase Chain Reaction
Poisons
Brain-Derived Neurotrophic Factor
Microglia
Hindlimb
Interleukin-1
Interleukin-4
Disease Progression
Anti-Inflammatory Agents
Extremities
Lymphocytes
T-Lymphocytes
Messenger RNA

Keywords

  • ALSIL-4
  • Inflammation
  • Microglia
  • Protective microglia
  • Spinal cord
  • Toxic microglia

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Beers, D. R., Zhao, W., Liao, B., Kano, O., Wang, J., Huang, A., ... Henkel, J. S. (2011). Neuroinflammation modulates distinct regional and temporal clinical responses in ALS mice. Brain, Behavior, and Immunity, 25(5), 1025-1035. https://doi.org/10.1016/j.bbi.2010.12.008

Neuroinflammation modulates distinct regional and temporal clinical responses in ALS mice. / Beers, David R.; Zhao, Weihua; Liao, Bing; Kano, Osamu; Wang, Jinghong; Huang, Ailing; Appel, Stanley H.; Henkel, Jenny S.

In: Brain, Behavior, and Immunity, Vol. 25, No. 5, 01.07.2011, p. 1025-1035.

Research output: Contribution to journalArticle

Beers, DR, Zhao, W, Liao, B, Kano, O, Wang, J, Huang, A, Appel, SH & Henkel, JS 2011, 'Neuroinflammation modulates distinct regional and temporal clinical responses in ALS mice', Brain, Behavior, and Immunity, vol. 25, no. 5, pp. 1025-1035. https://doi.org/10.1016/j.bbi.2010.12.008
Beers, David R. ; Zhao, Weihua ; Liao, Bing ; Kano, Osamu ; Wang, Jinghong ; Huang, Ailing ; Appel, Stanley H. ; Henkel, Jenny S. / Neuroinflammation modulates distinct regional and temporal clinical responses in ALS mice. In: Brain, Behavior, and Immunity. 2011 ; Vol. 25, No. 5. pp. 1025-1035.
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