Abstract
We used the RT-PCR technique and immunocytochemical methods to determine the expression of endothelial nitric oxide synthase (eNOS) or neuronal nitric oxide synthase (nNOS) in the cortical collecting duct (CCD) in rats on high- K+ diet. The microdissected CCDs of the rat kidney were lysed, and RT-PCR was carried out using rat nNOS and eNOS gene-specific primers. Southern analysis showed the presence of mRNA of nNOS but not eNOS in the CCD. The presence of nNOS in the CCD was further confirmed by light microscopy. We used the polyclonal nNOS antibody in immunocytochemical studies of the isolated CCD. We found that immunoreactivity to nNOS was present in the CCD and heterogeneous with positive and negative immunostaining. We performed the immunocytochemical studies in the split-open CCD and found that the immunoreactivity to nNOS was detected only in principal cells but not in intercalated cells. We conclude that nNOS is expressed in the rat CCD in rats on high-K+ diet. The presence of nNOS in the CCD is heterogeneous and mainly located in principal cells.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Renal Physiology |
| Volume | 275 |
| Issue number | 3 44-3 |
| State | Published - Sep 1 1998 |
| Externally published | Yes |
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Keywords
- Guanosine 3',5'-cyclic monophosphate
- Immunocytochemistry
- Reverse transcription-polymerase chain reaction
- Soluble guanylate cyclase
ASJC Scopus subject areas
- Physiology
- Urology
Cite this
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. / Wang, Xianhong; Lu, Ming; Gao, Yongzong; Papapetropoulos, Andreas; Sessa, William C.; Wang, Wenhui.
In: American Journal of Physiology - Renal Physiology, Vol. 275, No. 3 44-3, 01.09.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
AU - Wang, Xianhong
AU - Lu, Ming
AU - Gao, Yongzong
AU - Papapetropoulos, Andreas
AU - Sessa, William C.
AU - Wang, Wenhui
PY - 1998/9/1
Y1 - 1998/9/1
N2 - We used the RT-PCR technique and immunocytochemical methods to determine the expression of endothelial nitric oxide synthase (eNOS) or neuronal nitric oxide synthase (nNOS) in the cortical collecting duct (CCD) in rats on high- K+ diet. The microdissected CCDs of the rat kidney were lysed, and RT-PCR was carried out using rat nNOS and eNOS gene-specific primers. Southern analysis showed the presence of mRNA of nNOS but not eNOS in the CCD. The presence of nNOS in the CCD was further confirmed by light microscopy. We used the polyclonal nNOS antibody in immunocytochemical studies of the isolated CCD. We found that immunoreactivity to nNOS was present in the CCD and heterogeneous with positive and negative immunostaining. We performed the immunocytochemical studies in the split-open CCD and found that the immunoreactivity to nNOS was detected only in principal cells but not in intercalated cells. We conclude that nNOS is expressed in the rat CCD in rats on high-K+ diet. The presence of nNOS in the CCD is heterogeneous and mainly located in principal cells.
AB - We used the RT-PCR technique and immunocytochemical methods to determine the expression of endothelial nitric oxide synthase (eNOS) or neuronal nitric oxide synthase (nNOS) in the cortical collecting duct (CCD) in rats on high- K+ diet. The microdissected CCDs of the rat kidney were lysed, and RT-PCR was carried out using rat nNOS and eNOS gene-specific primers. Southern analysis showed the presence of mRNA of nNOS but not eNOS in the CCD. The presence of nNOS in the CCD was further confirmed by light microscopy. We used the polyclonal nNOS antibody in immunocytochemical studies of the isolated CCD. We found that immunoreactivity to nNOS was present in the CCD and heterogeneous with positive and negative immunostaining. We performed the immunocytochemical studies in the split-open CCD and found that the immunoreactivity to nNOS was detected only in principal cells but not in intercalated cells. We conclude that nNOS is expressed in the rat CCD in rats on high-K+ diet. The presence of nNOS in the CCD is heterogeneous and mainly located in principal cells.
KW - Guanosine 3',5'-cyclic monophosphate
KW - Immunocytochemistry
KW - Reverse transcription-polymerase chain reaction
KW - Soluble guanylate cyclase
UR - http://www.scopus.com/inward/record.url?scp=0031706296&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031706296&partnerID=8YFLogxK
M3 - Article
C2 - 9729512
AN - SCOPUS:0031706296
VL - 275
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 3 44-3
ER -