Neuronal nitric oxide synthase is expressed in principal cell of collecting duct

Xianhong Wang, Ming Lu, Yongzong Gao, Andreas Papapetropoulos, William C. Sessa, Wenhui Wang

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Abstract

We used the RT-PCR technique and immunocytochemical methods to determine the expression of endothelial nitric oxide synthase (eNOS) or neuronal nitric oxide synthase (nNOS) in the cortical collecting duct (CCD) in rats on high- K+ diet. The microdissected CCDs of the rat kidney were lysed, and RT-PCR was carried out using rat nNOS and eNOS gene-specific primers. Southern analysis showed the presence of mRNA of nNOS but not eNOS in the CCD. The presence of nNOS in the CCD was further confirmed by light microscopy. We used the polyclonal nNOS antibody in immunocytochemical studies of the isolated CCD. We found that immunoreactivity to nNOS was present in the CCD and heterogeneous with positive and negative immunostaining. We performed the immunocytochemical studies in the split-open CCD and found that the immunoreactivity to nNOS was detected only in principal cells but not in intercalated cells. We conclude that nNOS is expressed in the rat CCD in rats on high-K+ diet. The presence of nNOS in the CCD is heterogeneous and mainly located in principal cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume275
Issue number3 44-3
StatePublished - Sep 1 1998
Externally publishedYes

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Keywords

  • Guanosine 3',5'-cyclic monophosphate
  • Immunocytochemistry
  • Reverse transcription-polymerase chain reaction
  • Soluble guanylate cyclase

ASJC Scopus subject areas

  • Physiology
  • Urology

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