Neuropeptides in Heteroptera

Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry

Susanne Neupert, William Russell, David H. Russell, Juan D. López, Reinhard Predel, Ronald J. Nachman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalPeptides
Volume30
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

Heteroptera
Tachykinins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Neuropeptides
Mass spectrometry
Mass Spectrometry
Peptides
allatotropin
Locusta migratoria
Nerve Tissue
Hemiptera
Accessories
Neurology
Tandem Mass Spectrometry
Insects

Keywords

  • Acrosternum hilare
  • Allatotropin-related peptide
  • Banasa dimiata
  • Euschistus servus
  • Heteroptera
  • Insect neuropeptides
  • Mass spectrometry
  • Nezara viridula
  • Oncopeltus fasciatus
  • Pentatoma rufipes
  • Pyrrhocoris apterus
  • Tachykinin-related peptides

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Neuropeptides in Heteroptera : Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry. / Neupert, Susanne; Russell, William; Russell, David H.; López, Juan D.; Predel, Reinhard; Nachman, Ronald J.

In: Peptides, Vol. 30, No. 3, 03.2009, p. 483-488.

Research output: Contribution to journalArticle

Neupert, Susanne ; Russell, William ; Russell, David H. ; López, Juan D. ; Predel, Reinhard ; Nachman, Ronald J. / Neuropeptides in Heteroptera : Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry. In: Peptides. 2009 ; Vol. 30, No. 3. pp. 483-488.
@article{e2de9ad65dc44a278bda6f2ea02cd33a,
title = "Neuropeptides in Heteroptera: Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry",
abstract = "Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.",
keywords = "Acrosternum hilare, Allatotropin-related peptide, Banasa dimiata, Euschistus servus, Heteroptera, Insect neuropeptides, Mass spectrometry, Nezara viridula, Oncopeltus fasciatus, Pentatoma rufipes, Pyrrhocoris apterus, Tachykinin-related peptides",
author = "Susanne Neupert and William Russell and Russell, {David H.} and L{\'o}pez, {Juan D.} and Reinhard Predel and Nachman, {Ronald J.}",
year = "2009",
month = "3",
doi = "10.1016/j.peptides.2008.11.009",
language = "English (US)",
volume = "30",
pages = "483--488",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Neuropeptides in Heteroptera

T2 - Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry

AU - Neupert, Susanne

AU - Russell, William

AU - Russell, David H.

AU - López, Juan D.

AU - Predel, Reinhard

AU - Nachman, Ronald J.

PY - 2009/3

Y1 - 2009/3

N2 - Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.

AB - Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.

KW - Acrosternum hilare

KW - Allatotropin-related peptide

KW - Banasa dimiata

KW - Euschistus servus

KW - Heteroptera

KW - Insect neuropeptides

KW - Mass spectrometry

KW - Nezara viridula

KW - Oncopeltus fasciatus

KW - Pentatoma rufipes

KW - Pyrrhocoris apterus

KW - Tachykinin-related peptides

UR - http://www.scopus.com/inward/record.url?scp=60749084663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60749084663&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2008.11.009

DO - 10.1016/j.peptides.2008.11.009

M3 - Article

VL - 30

SP - 483

EP - 488

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 3

ER -