Neuropeptides in Heteroptera: Identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry

Susanne Neupert, William K. Russell, David H. Russell, Juan D. López, Reinhard Predel, Ronald J. Nachman

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalPeptides
Volume30
Issue number3
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Keywords

  • Acrosternum hilare
  • Allatotropin-related peptide
  • Banasa dimiata
  • Euschistus servus
  • Heteroptera
  • Insect neuropeptides
  • Mass spectrometry
  • Nezara viridula
  • Oncopeltus fasciatus
  • Pentatoma rufipes
  • Pyrrhocoris apterus
  • Tachykinin-related peptides

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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