Neuropeptidomics of the mosquito Aedes aegypti

Reinhard Predel, Susanne Neupert, Stephen F. Garczynski, Joe W. Crim, Mark R. Brown, William Russell, Jǒrg Kahnt, David H. Russell, Ronald J. Nachman

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Neuropeptidomic data were collected on the mosquito Ae. aegypti, which is considered the most tractable mosquito species for physiological and endocrine studies. The data were solely obtained by direct mass spectrometric profiling, including tandem fragmentation, of selected tissues from single specimens, which yielded a largely complete accounting of the putative bioactive neuropeptides; truncated neuropeptides with low abundance were not counted as mature peptides. Differential processing within the CNS was detected for the CAPA-precursor, and differential post-translational processing (pyroglutamate formation) was detected for AST-C and CAPA-PVK-2. For the first time in insects, we succeeded in the direct mass spectrometric profiling of midgut tissue which yielded a comprehensive and immediate overview of the peptides involved in the endocrine system of the gut. Head peptides which were earlier identified as the most abundant RFamides of Ae. aegypti, were not detected in any part of the CNS or midgut. This study provides a framework for future investigations on mosquito endocrinology and neurobiology. Given the high sequence similarity of neuropeptide precursors identified in other medically important mosquitoes, conclusions regarding the peptidome of Ae. aegypti likely are applicable to these mosquitoes.

Original languageEnglish (US)
Pages (from-to)2006-2015
Number of pages10
JournalJournal of Proteome Research
Volume9
Issue number4
DOIs
StatePublished - Apr 5 2010
Externally publishedYes

Fingerprint

Aedes
Culicidae
Neuropeptides
Peptides
Endocrinology
Pyrrolidonecarboxylic Acid
Tissue
Processing
Endocrine System
Neurobiology
Insects
Head

Keywords

  • Aedes aegypti
  • CAPA-peptides
  • Culicidae
  • Insect neuropeptides
  • MALDI-TOF
  • Midgut
  • Peptidomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Predel, R., Neupert, S., Garczynski, S. F., Crim, J. W., Brown, M. R., Russell, W., ... Nachman, R. J. (2010). Neuropeptidomics of the mosquito Aedes aegypti. Journal of Proteome Research, 9(4), 2006-2015. https://doi.org/10.1021/pr901187p

Neuropeptidomics of the mosquito Aedes aegypti. / Predel, Reinhard; Neupert, Susanne; Garczynski, Stephen F.; Crim, Joe W.; Brown, Mark R.; Russell, William; Kahnt, Jǒrg; Russell, David H.; Nachman, Ronald J.

In: Journal of Proteome Research, Vol. 9, No. 4, 05.04.2010, p. 2006-2015.

Research output: Contribution to journalArticle

Predel, R, Neupert, S, Garczynski, SF, Crim, JW, Brown, MR, Russell, W, Kahnt, J, Russell, DH & Nachman, RJ 2010, 'Neuropeptidomics of the mosquito Aedes aegypti', Journal of Proteome Research, vol. 9, no. 4, pp. 2006-2015. https://doi.org/10.1021/pr901187p
Predel R, Neupert S, Garczynski SF, Crim JW, Brown MR, Russell W et al. Neuropeptidomics of the mosquito Aedes aegypti. Journal of Proteome Research. 2010 Apr 5;9(4):2006-2015. https://doi.org/10.1021/pr901187p
Predel, Reinhard ; Neupert, Susanne ; Garczynski, Stephen F. ; Crim, Joe W. ; Brown, Mark R. ; Russell, William ; Kahnt, Jǒrg ; Russell, David H. ; Nachman, Ronald J. / Neuropeptidomics of the mosquito Aedes aegypti. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 4. pp. 2006-2015.
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