Neurotensin augments intestinal regeneration after small bowel resection in rats

Masaski Izukura, B. Mark Evers, Dilipkumar Parekh, Keigo Yoshinaga, Tatsuo Uchida, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Massive small bowel resection (SBR) is characterized by increased proliferation of residual gut mucosa and pancreas. Neurotensin (NT), a gut tridecapeptide, stimulates growth of normal gut mucosa and pancreas. This study examined whether NT affected growth of the small intestine and the pancreas after either distal or proximal SBR. Male Fischer 344 rats were divided into four groups. Group 1 underwent ileal transection with reanastomosis (SHAM) and group 2 underwent 70% distal SBR. Group 3 underwent SHAM operation (jejunal transection), and group 4 underwent 70% proximal SBR. After operation, each group was further subdivided to receive either saline (control) or NT (300 μg/kg) subcutaneously in gelatin every 8 hours for 7 days. At death, the pancreas and proximal jejunum (from groups 1 and 2) or distal ileum (from groups 3 and 4) were removed, weighed, and analyzed for DNA, RNA, and protein content. Both proximal and distal SBR significantly increased mucosal growth in the remnant intestine; a more pronounced effect was noted with proximal SBR. Administration of NT significantly augmented the adaptive changes in both groups of rats by mechanisms involving increases in both cell size (hypertrophy) and cell number (hyperplasia). Pancreatic growth was stimulated by distal (but not proximal) SBR; NT did not augment this response. The authors conclude that NT augments intestinal growth after SBR by mechanisms involving an increase in overall mucosal cellularity. Administration of NT may be therapeutically useful to enhance mucosal regeneration during the early period of adaptive hyperplasia after SBR.

Original languageEnglish (US)
Pages (from-to)520-526
Number of pages7
JournalAnnals of Surgery
Volume215
Issue number5
StatePublished - 1992

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Neurotensin
Regeneration
Pancreas
Growth
Hyperplasia
Mucous Membrane
Inbred F344 Rats
Jejunum
Gelatin
Cell Size
Ileum
Hypertrophy
Small Intestine
Intestines
Cell Count
RNA
DNA

ASJC Scopus subject areas

  • Surgery

Cite this

Izukura, M., Evers, B. M., Parekh, D., Yoshinaga, K., Uchida, T., Townsend, C., & Thompson, J. C. (1992). Neurotensin augments intestinal regeneration after small bowel resection in rats. Annals of Surgery, 215(5), 520-526.

Neurotensin augments intestinal regeneration after small bowel resection in rats. / Izukura, Masaski; Evers, B. Mark; Parekh, Dilipkumar; Yoshinaga, Keigo; Uchida, Tatsuo; Townsend, Courtney; Thompson, James C.

In: Annals of Surgery, Vol. 215, No. 5, 1992, p. 520-526.

Research output: Contribution to journalArticle

Izukura, M, Evers, BM, Parekh, D, Yoshinaga, K, Uchida, T, Townsend, C & Thompson, JC 1992, 'Neurotensin augments intestinal regeneration after small bowel resection in rats', Annals of Surgery, vol. 215, no. 5, pp. 520-526.
Izukura M, Evers BM, Parekh D, Yoshinaga K, Uchida T, Townsend C et al. Neurotensin augments intestinal regeneration after small bowel resection in rats. Annals of Surgery. 1992;215(5):520-526.
Izukura, Masaski ; Evers, B. Mark ; Parekh, Dilipkumar ; Yoshinaga, Keigo ; Uchida, Tatsuo ; Townsend, Courtney ; Thompson, James C. / Neurotensin augments intestinal regeneration after small bowel resection in rats. In: Annals of Surgery. 1992 ; Vol. 215, No. 5. pp. 520-526.
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