Neurotensin expression and release in human colon cancers

B. Mark Evers, Jin Ishizuka, Dai H. Chung, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Neurotensin (NT), a distal gut peptide released by intraluminal fats, is trophic for normal small bowel and colonic mucosa. In addition, NT stimulates growth of certain colon cancers; the mechanism for this effect is not known. The purpose of this study was to determine whether human colon cancers (HCC) (1) express the mRNA for NT/neuromedin N (N), (2) produce NT peptide. and (3) express the mRNA for a functional NT receptor (NTR). RNA was extracted from four HCC cell lines in culture, nine HCC lines established in athymic nude mice, and from six HCC and adjacent normal mucosa from freshly resected operative specimens; the RNA was analyzed for NT/N mRNA by Northern hybridization with a complementary DNA probe. Neurotensin peptide content, NTR expression, and intracellular Ca+ ([Ca++]i) mobilization in response to NT were evaluated in three HCC cell lines (LoVo, HT29, HCT116). Neurotensin/N mRNA transcripts were identified in all four of the HCC cell lines and in one of nine HCC in nude mice. Neurotensin expression was found in two of six freshly resected HCC and in none of the six corresponding samples of normal mucosa. Neurotensin peptide was identified by RIA in LoVo, HT29, and HCT116. In addition, NTR mRNA was found in HT29 and HCT116. Neurotensin stimulated [Ca++]i mobilization in HCT116 (without serum) and in LoVo (with 0.25% serum). These findings demonstrate the presence of NT/N mRNA and NT peptide and the presence of a functional NTR in certain HCC. Neurotensin, a potent trophic factor for normal gut mucosa, may function as an autocrine growth factor in certain human colon cancers.

Original languageEnglish (US)
Pages (from-to)423-430
Number of pages8
JournalAnnals of Surgery
Volume216
Issue number4
StatePublished - 1992

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Neurotensin
Colonic Neoplasms
Messenger RNA
Mucous Membrane
Peptides
Nude Mice
neuromedin N
proctolin
Cell Line
Neurotensin Receptors
RNA
DNA Probes
Serum

ASJC Scopus subject areas

  • Surgery

Cite this

Mark Evers, B., Ishizuka, J., Chung, D. H., Townsend, C., & Thompson, J. C. (1992). Neurotensin expression and release in human colon cancers. Annals of Surgery, 216(4), 423-430.

Neurotensin expression and release in human colon cancers. / Mark Evers, B.; Ishizuka, Jin; Chung, Dai H.; Townsend, Courtney; Thompson, James C.

In: Annals of Surgery, Vol. 216, No. 4, 1992, p. 423-430.

Research output: Contribution to journalArticle

Mark Evers, B, Ishizuka, J, Chung, DH, Townsend, C & Thompson, JC 1992, 'Neurotensin expression and release in human colon cancers', Annals of Surgery, vol. 216, no. 4, pp. 423-430.
Mark Evers B, Ishizuka J, Chung DH, Townsend C, Thompson JC. Neurotensin expression and release in human colon cancers. Annals of Surgery. 1992;216(4):423-430.
Mark Evers, B. ; Ishizuka, Jin ; Chung, Dai H. ; Townsend, Courtney ; Thompson, James C. / Neurotensin expression and release in human colon cancers. In: Annals of Surgery. 1992 ; Vol. 216, No. 4. pp. 423-430.
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