Neurotensin regulates growth of human pancreatic cancer

Jin Ishizuka, Courtney Townsend, James C. Thompson

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objective: The effect of neurotensin (NT) on in vitro growth of human pancreatic cancer cells (MIA PaCa-2) was examined. Furthermore, the intracellular signal-transduction pathways by which neurotensin regulates growth of MIA PaCa-2 cells were determined. Summary Background Data: NT is trophic for normal rat pancreas, but the effect of NT on growth of human pancreatic cancer is not known. Methods: Effects of NT (10-12 to 10-6 mol/l) on growth of MIA PaCa-2 cells were determined by both count of cell numbers and 3H-thymidine incorporation. Action of NT on phosphatidylinositol (Pl) hydrolysis, cyclic AMP production, and intracellular calcium level were determined by conventional methods. The effects of 8-bromo-cyclic AMP and prostaglandin E2 on cell growth were determined. Results: Low concentrations of NT (10-12 to 10-9 mol/l) stimulated growth in a dose-dependent manner, but higher concentrations of NT (10-8 to 10-6 mol/l) did not stimulate growth of MIA PaCa-2 cells. NT (10-12 to 10-6 mol/l) stimulated Pl hydrolysis and increased intracellular calcium levels in a dose-dependent manner. High concentrations of NT (10-8 to 10-6 mol/l) stimulated production of cyclic AMP in a dose-dependent manner. 8-bromo-cyclic AMP inhibited growth of MIA PaCa-2 cells; prostaglandin E2 did not affect growth of MIA PaCa-2 cells. Conclusions: NT stimulates growth of MIA PaCa-2 cells through stimulation of Pl hydrolysis and mobilization of calcium. Stimulation of the cyclic AMP pathway by high concentrations of NT abolishes the growth-stimulatory effect of NT that is mediated through Pl hydrolysis or calcium mobilization.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalAnnals of Surgery
Volume217
Issue number5
StatePublished - May 1993

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Neurotensin
Pancreatic Neoplasms
Growth
Phosphatidylinositols
Hydrolysis
Cyclic AMP
Calcium
8-Bromo Cyclic Adenosine Monophosphate
Dinoprostone
Thymidine
Pancreas
Signal Transduction

ASJC Scopus subject areas

  • Surgery

Cite this

Ishizuka, J., Townsend, C., & Thompson, J. C. (1993). Neurotensin regulates growth of human pancreatic cancer. Annals of Surgery, 217(5), 439-446.

Neurotensin regulates growth of human pancreatic cancer. / Ishizuka, Jin; Townsend, Courtney; Thompson, James C.

In: Annals of Surgery, Vol. 217, No. 5, 05.1993, p. 439-446.

Research output: Contribution to journalArticle

Ishizuka, J, Townsend, C & Thompson, JC 1993, 'Neurotensin regulates growth of human pancreatic cancer', Annals of Surgery, vol. 217, no. 5, pp. 439-446.
Ishizuka, Jin ; Townsend, Courtney ; Thompson, James C. / Neurotensin regulates growth of human pancreatic cancer. In: Annals of Surgery. 1993 ; Vol. 217, No. 5. pp. 439-446.
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