Neurotensin stimulates growth of colon cancer

K. Yoshinaga, B. M. Evers, M. Izukura, D. Parekh, T. Uchida, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Neurotensin (NT), a peptide from the distal gut that is released by fat ingestion, stimulates the growth of normal small bowel and colonic mucosa. The purpose of this study was to determine whether chronic administration of NT would affect the growth of a mouse colon cancer (MC-26) and a human colon cancer (LoVo) in vivo. In experiment 1, male Balb/c mice were inoculated with MC-26 cells (5 × 104) and then randomized to four treatment groups receiving either saline (control) or NT (150, 300 or 600 μg kg-1) administered subcutaneously (s.c.) every 8 h for 21 days. In experiment 2, 60 mice with MC-26 tumours were randomized to receive saline (control) or NT (300 or 600 μg kg-1) for 28 days, and survival was then assessed. In experiment 3, 16 athymic nude mice with LoVo tumour xenografts were randomized to receive either saline (control) or NT (600 μg kg-1). We found that administration of NT (300 and 600 μg kg-1) significantly stimulated mean tumour area, weight and DNA, RNA and protein content of MC-26 tumours. In addition, the survival rate of mice bearing MC-26 tumours and treated with either dose of NT was significantly decreased compared with the control group given saline injections. Similarly, NT (600 μg kg-1) stimulated growth (tumour area, weight and nucleic acid contents) of the human colon cancer, LoVo. We conclude that NT acts as a trophic factor for the colon cancer cell lines MC-26 and LoVo in vivo. NT may play an important role in growth regulation of certain colon cancers.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalSurgical Oncology
Volume1
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Neurotensin
Colonic Neoplasms
Growth
Tumor Burden
Nude Mice
Neoplasms
Heterografts
Nucleic Acids
Mucous Membrane
Eating
Fats
RNA
Cell Line

Keywords

  • colon cancer
  • neurotensin

ASJC Scopus subject areas

  • Oncology
  • Surgery

Cite this

Yoshinaga, K., Evers, B. M., Izukura, M., Parekh, D., Uchida, T., Townsend, C., & Thompson, J. C. (1992). Neurotensin stimulates growth of colon cancer. Surgical Oncology, 1(2), 127-134. https://doi.org/10.1016/0960-7404(92)90025-G

Neurotensin stimulates growth of colon cancer. / Yoshinaga, K.; Evers, B. M.; Izukura, M.; Parekh, D.; Uchida, T.; Townsend, Courtney; Thompson, J. C.

In: Surgical Oncology, Vol. 1, No. 2, 1992, p. 127-134.

Research output: Contribution to journalArticle

Yoshinaga, K, Evers, BM, Izukura, M, Parekh, D, Uchida, T, Townsend, C & Thompson, JC 1992, 'Neurotensin stimulates growth of colon cancer', Surgical Oncology, vol. 1, no. 2, pp. 127-134. https://doi.org/10.1016/0960-7404(92)90025-G
Yoshinaga K, Evers BM, Izukura M, Parekh D, Uchida T, Townsend C et al. Neurotensin stimulates growth of colon cancer. Surgical Oncology. 1992;1(2):127-134. https://doi.org/10.1016/0960-7404(92)90025-G
Yoshinaga, K. ; Evers, B. M. ; Izukura, M. ; Parekh, D. ; Uchida, T. ; Townsend, Courtney ; Thompson, J. C. / Neurotensin stimulates growth of colon cancer. In: Surgical Oncology. 1992 ; Vol. 1, No. 2. pp. 127-134.
@article{c6bcc2c632d94517b29c01daa679bf3f,
title = "Neurotensin stimulates growth of colon cancer",
abstract = "Neurotensin (NT), a peptide from the distal gut that is released by fat ingestion, stimulates the growth of normal small bowel and colonic mucosa. The purpose of this study was to determine whether chronic administration of NT would affect the growth of a mouse colon cancer (MC-26) and a human colon cancer (LoVo) in vivo. In experiment 1, male Balb/c mice were inoculated with MC-26 cells (5 × 104) and then randomized to four treatment groups receiving either saline (control) or NT (150, 300 or 600 μg kg-1) administered subcutaneously (s.c.) every 8 h for 21 days. In experiment 2, 60 mice with MC-26 tumours were randomized to receive saline (control) or NT (300 or 600 μg kg-1) for 28 days, and survival was then assessed. In experiment 3, 16 athymic nude mice with LoVo tumour xenografts were randomized to receive either saline (control) or NT (600 μg kg-1). We found that administration of NT (300 and 600 μg kg-1) significantly stimulated mean tumour area, weight and DNA, RNA and protein content of MC-26 tumours. In addition, the survival rate of mice bearing MC-26 tumours and treated with either dose of NT was significantly decreased compared with the control group given saline injections. Similarly, NT (600 μg kg-1) stimulated growth (tumour area, weight and nucleic acid contents) of the human colon cancer, LoVo. We conclude that NT acts as a trophic factor for the colon cancer cell lines MC-26 and LoVo in vivo. NT may play an important role in growth regulation of certain colon cancers.",
keywords = "colon cancer, neurotensin",
author = "K. Yoshinaga and Evers, {B. M.} and M. Izukura and D. Parekh and T. Uchida and Courtney Townsend and Thompson, {J. C.}",
year = "1992",
doi = "10.1016/0960-7404(92)90025-G",
language = "English (US)",
volume = "1",
pages = "127--134",
journal = "Surgical Oncology",
issn = "0960-7404",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Neurotensin stimulates growth of colon cancer

AU - Yoshinaga, K.

AU - Evers, B. M.

AU - Izukura, M.

AU - Parekh, D.

AU - Uchida, T.

AU - Townsend, Courtney

AU - Thompson, J. C.

PY - 1992

Y1 - 1992

N2 - Neurotensin (NT), a peptide from the distal gut that is released by fat ingestion, stimulates the growth of normal small bowel and colonic mucosa. The purpose of this study was to determine whether chronic administration of NT would affect the growth of a mouse colon cancer (MC-26) and a human colon cancer (LoVo) in vivo. In experiment 1, male Balb/c mice were inoculated with MC-26 cells (5 × 104) and then randomized to four treatment groups receiving either saline (control) or NT (150, 300 or 600 μg kg-1) administered subcutaneously (s.c.) every 8 h for 21 days. In experiment 2, 60 mice with MC-26 tumours were randomized to receive saline (control) or NT (300 or 600 μg kg-1) for 28 days, and survival was then assessed. In experiment 3, 16 athymic nude mice with LoVo tumour xenografts were randomized to receive either saline (control) or NT (600 μg kg-1). We found that administration of NT (300 and 600 μg kg-1) significantly stimulated mean tumour area, weight and DNA, RNA and protein content of MC-26 tumours. In addition, the survival rate of mice bearing MC-26 tumours and treated with either dose of NT was significantly decreased compared with the control group given saline injections. Similarly, NT (600 μg kg-1) stimulated growth (tumour area, weight and nucleic acid contents) of the human colon cancer, LoVo. We conclude that NT acts as a trophic factor for the colon cancer cell lines MC-26 and LoVo in vivo. NT may play an important role in growth regulation of certain colon cancers.

AB - Neurotensin (NT), a peptide from the distal gut that is released by fat ingestion, stimulates the growth of normal small bowel and colonic mucosa. The purpose of this study was to determine whether chronic administration of NT would affect the growth of a mouse colon cancer (MC-26) and a human colon cancer (LoVo) in vivo. In experiment 1, male Balb/c mice were inoculated with MC-26 cells (5 × 104) and then randomized to four treatment groups receiving either saline (control) or NT (150, 300 or 600 μg kg-1) administered subcutaneously (s.c.) every 8 h for 21 days. In experiment 2, 60 mice with MC-26 tumours were randomized to receive saline (control) or NT (300 or 600 μg kg-1) for 28 days, and survival was then assessed. In experiment 3, 16 athymic nude mice with LoVo tumour xenografts were randomized to receive either saline (control) or NT (600 μg kg-1). We found that administration of NT (300 and 600 μg kg-1) significantly stimulated mean tumour area, weight and DNA, RNA and protein content of MC-26 tumours. In addition, the survival rate of mice bearing MC-26 tumours and treated with either dose of NT was significantly decreased compared with the control group given saline injections. Similarly, NT (600 μg kg-1) stimulated growth (tumour area, weight and nucleic acid contents) of the human colon cancer, LoVo. We conclude that NT acts as a trophic factor for the colon cancer cell lines MC-26 and LoVo in vivo. NT may play an important role in growth regulation of certain colon cancers.

KW - colon cancer

KW - neurotensin

UR - http://www.scopus.com/inward/record.url?scp=0026702097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026702097&partnerID=8YFLogxK

U2 - 10.1016/0960-7404(92)90025-G

DO - 10.1016/0960-7404(92)90025-G

M3 - Article

VL - 1

SP - 127

EP - 134

JO - Surgical Oncology

JF - Surgical Oncology

SN - 0960-7404

IS - 2

ER -