Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide

Differential requirements for serum and CD14

Ana Maria Soler-Rodriguez, Hongwei Zhang, Henri S. Lichenstein, Nilofer Qureshi, David Niesel, Sheila E. Crowe, Johnny Peterson, Gary R. Kumpel

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Neutrophil activation plays an important role in the inflammatory response to Gram-negative bacterial infections. LPS has been shown to be a major mediator of neutrophil activation which is accompanied by an early down-regulation of L-selectin and Up-regulation of CD1lb/CD18. In this study, we investigated whether lipoprotein (LP), the most abundant protein in the outer membrane of bacteria from the family Enterobacteriaceae, can activate neutrophils and whether this activation is mediated by mechanisms that differ from those used by LPS or Escherichia coli diphosphoryl lipid A (EcDPLA). Neutrophil activation was assessed by measuring down-regulation of L-selectin and up-regulation of CD11b/CD18. When comparing molar concentrations of LP vs EcDPLA, LP was more potent (four times) at activating neutrophils. In contrast to LPS/EcDPLA, LP activation of neutrophils was serum independent. However, LP activation of neutrophils was enhanced by the addition of soluble CD14 and/or LPS-binding protein. In the presence of serum, LP activation of neutrophils was inhibited by different mAbs to CD14. This inhibition was significantly reduced or absent when performed in the absence of serum. Diphosphoryl lipid A from Rhodobacter spheroides (RaDPLA) completely inhibited LPS/EcDPLA activation of neutrophils but only slightly inhibited LP activation of neutrophils. These results suggest that LP activation of human neutrophils can be mediated by a mechanism that is different from LPS activation and that LP is a potentially important component in the development of diseases caused by Gram-negative bacteria of the family Enterobacteriaceae.

Original languageEnglish (US)
Pages (from-to)2674-2683
Number of pages10
JournalJournal of Immunology
Volume164
Issue number5
StatePublished - Mar 1 2000

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Neutrophil Activation
Lipoproteins
Lipopolysaccharides
Serum
Escherichia coli
L-Selectin
Enterobacteriaceae
Up-Regulation
Down-Regulation
Gram-Negative Bacterial Infections
Rhodobacter sphaeroides
Gram-Negative Bacteria
Neutrophils
diphosphoryl lipid A
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Soler-Rodriguez, A. M., Zhang, H., Lichenstein, H. S., Qureshi, N., Niesel, D., Crowe, S. E., ... Kumpel, G. R. (2000). Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide: Differential requirements for serum and CD14. Journal of Immunology, 164(5), 2674-2683.

Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide : Differential requirements for serum and CD14. / Soler-Rodriguez, Ana Maria; Zhang, Hongwei; Lichenstein, Henri S.; Qureshi, Nilofer; Niesel, David; Crowe, Sheila E.; Peterson, Johnny; Kumpel, Gary R.

In: Journal of Immunology, Vol. 164, No. 5, 01.03.2000, p. 2674-2683.

Research output: Contribution to journalArticle

Soler-Rodriguez, AM, Zhang, H, Lichenstein, HS, Qureshi, N, Niesel, D, Crowe, SE, Peterson, J & Kumpel, GR 2000, 'Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide: Differential requirements for serum and CD14', Journal of Immunology, vol. 164, no. 5, pp. 2674-2683.
Soler-Rodriguez AM, Zhang H, Lichenstein HS, Qureshi N, Niesel D, Crowe SE et al. Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide: Differential requirements for serum and CD14. Journal of Immunology. 2000 Mar 1;164(5):2674-2683.
Soler-Rodriguez, Ana Maria ; Zhang, Hongwei ; Lichenstein, Henri S. ; Qureshi, Nilofer ; Niesel, David ; Crowe, Sheila E. ; Peterson, Johnny ; Kumpel, Gary R. / Neutrophil activation by bacterial lipoprotein versus lipopolysaccharide : Differential requirements for serum and CD14. In: Journal of Immunology. 2000 ; Vol. 164, No. 5. pp. 2674-2683.
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