Neutrophil activation by monomeric interleukin-8

Krishna Rajarathnam, Brian D. Sykes, Cyril M. Kay, Beatrice Dewald, Thomas Geiser, Marco Baggiolini, Ian Clark-Lewis

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.

Original languageEnglish (US)
Pages (from-to)90-92
Number of pages3
JournalScience
Volume264
Issue number5155
StatePublished - Apr 1 1994
Externally publishedYes

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Neutrophil Activation
Interleukin-8
Magnetic Resonance Spectroscopy
Ultracentrifugation
Dimerization
Amides
Leucine
Hydrogen
Nitrogen
X-Rays
Proteins

ASJC Scopus subject areas

  • General

Cite this

Rajarathnam, K., Sykes, B. D., Kay, C. M., Dewald, B., Geiser, T., Baggiolini, M., & Clark-Lewis, I. (1994). Neutrophil activation by monomeric interleukin-8. Science, 264(5155), 90-92.

Neutrophil activation by monomeric interleukin-8. / Rajarathnam, Krishna; Sykes, Brian D.; Kay, Cyril M.; Dewald, Beatrice; Geiser, Thomas; Baggiolini, Marco; Clark-Lewis, Ian.

In: Science, Vol. 264, No. 5155, 01.04.1994, p. 90-92.

Research output: Contribution to journalArticle

Rajarathnam, K, Sykes, BD, Kay, CM, Dewald, B, Geiser, T, Baggiolini, M & Clark-Lewis, I 1994, 'Neutrophil activation by monomeric interleukin-8', Science, vol. 264, no. 5155, pp. 90-92.
Rajarathnam K, Sykes BD, Kay CM, Dewald B, Geiser T, Baggiolini M et al. Neutrophil activation by monomeric interleukin-8. Science. 1994 Apr 1;264(5155):90-92.
Rajarathnam, Krishna ; Sykes, Brian D. ; Kay, Cyril M. ; Dewald, Beatrice ; Geiser, Thomas ; Baggiolini, Marco ; Clark-Lewis, Ian. / Neutrophil activation by monomeric interleukin-8. In: Science. 1994 ; Vol. 264, No. 5155. pp. 90-92.
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