Neutrophil activation by monomeric interleukin-8

Krishnakumar Rajarathnam, Brian D. Sykes, Cyril M. Kay, Beatrice Dewald, Thomas Geiser, Marco Baggiolini, Ian Clark-Lewis

Research output: Contribution to journalArticlepeer-review

278 Scopus citations

Abstract

Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.

Original languageEnglish (US)
Pages (from-to)90-92
Number of pages3
JournalScience
Volume264
Issue number5155
DOIs
StatePublished - Apr 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

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