Neutrophil adhesion molecule expression in the developing neonatal rat exposed to hyperoxia

Susan Gerik, Susan E. Keeney, Dara V. Dallas, Kimberly H. Palkowetz, Frank C. Schmalstieg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neonatal rats have an increased tolerance to hyperoxia, which is associated with a diminished pulmonary inflammatory response compared with adults. To investigate this differing response, expression of the neutrophil adhesion molecules, L-selectin and CD18, and levels of soluble L-selectin, were examined using flow cytometry and sandwich enzyme-linked immunosorbent assay on air-exposed neonatal rat neutrophils at 0-24 and 72 h and 7, 10, 14, and 21 d of age compared with the adult and after exposure to hyperoxia (≥ 98% O 2) for 56 h in adults and for 72 h and 7 d in neonates. Expression of L-selectin in 0-24-h neonates was similar to adults, but was significantly lower than adults at 72 h and 7 d (P = 0.011). Soluble L-selectin levels were significantly higher than those in adults in the 0-24- and 72-h neonates (P < 0.001). CD18 expression in unstimulated and activated neutrophils of neonatal rats was higher at 0-24 h than in the adult (P < 0.001), but thereafter did not differ from adults. After hyperoxic exposure, L-selectin did not differ between the exposure groups but soluble L-selectin tended to increase in neonates after 7 d of O2 exposure Finally, CD18 was significantly higher after hyperoxic exposure of the adult (P = 0.008), but did not change with oxygen exposure in the neonate. Based on these findings, we speculate that differences between neonatal and adult rats in expression of L-selectin may contribute to delayed oxygen toxicity in neonatal rats.

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume29
Issue number4
DOIs
StatePublished - Oct 1 2003

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L-Selectin
Hyperoxia
Rats
Neutrophils
Adhesion
Molecules
Oxygen
Immunosorbents
Flow cytometry
Toxicity
Assays
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Air
Lung
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Neutrophil adhesion molecule expression in the developing neonatal rat exposed to hyperoxia. / Gerik, Susan; Keeney, Susan E.; Dallas, Dara V.; Palkowetz, Kimberly H.; Schmalstieg, Frank C.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 29, No. 4, 01.10.2003, p. 506-512.

Research output: Contribution to journalArticle

Gerik, Susan ; Keeney, Susan E. ; Dallas, Dara V. ; Palkowetz, Kimberly H. ; Schmalstieg, Frank C. / Neutrophil adhesion molecule expression in the developing neonatal rat exposed to hyperoxia. In: American Journal of Respiratory Cell and Molecular Biology. 2003 ; Vol. 29, No. 4. pp. 506-512.
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