New approach for measuring protrusive forces in cells

A. Mathur, P. Roca-Cusachs, O. M. Rossier, S. J. Wind, Michael Sheetz, J. Hone

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Forces experienced by cells internally and externally can alter cellular signaling and function. The authors have developed an elastomeric device for the measurement of protrusive forces generated by cells, in which cells spreading on a planar surface encounter an array of pillars. The protrusive force can be calculated from the deflection of the pillar using simple mechanical considerations. The advantage of this approach is that multiple sensors can be integrated into a single device. Theaverage protrusive force was calculated as 800 pN for NIH 3T3 fibroblasts. The correct quantification of protrusive forces potentially can be used in the development of novel cancer therapies and diagnostic tools.

Original languageEnglish (US)
Article number06FA02
JournalJournal of Vacuum Science and Technology B:Nanotechnology and Microelectronics
Volume29
Issue number6
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

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Cell signaling
Fibroblasts
Sensors
cells
fibroblasts
encounters
deflection
therapy
cancer
elastomeric
sensors

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

New approach for measuring protrusive forces in cells. / Mathur, A.; Roca-Cusachs, P.; Rossier, O. M.; Wind, S. J.; Sheetz, Michael; Hone, J.

In: Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics, Vol. 29, No. 6, 06FA02, 01.01.2011.

Research output: Contribution to journalArticle

Mathur, A. ; Roca-Cusachs, P. ; Rossier, O. M. ; Wind, S. J. ; Sheetz, Michael ; Hone, J. / New approach for measuring protrusive forces in cells. In: Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics. 2011 ; Vol. 29, No. 6.
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