New approach for measuring protrusive forces in cells

A. Mathur; P. Roca-Cusachs; O. M. Rossier; S. J. Wind; M. P. Sheetz; J. Hone

doi:10.1116/1.3655580

New approach for measuring protrusive forces in cells

A. Mathur, P. Roca-Cusachs, O. M. Rossier, S. J. Wind, M. P. Sheetz, J. Hone

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Forces experienced by cells internally and externally can alter cellular signaling and function. The authors have developed an elastomeric device for the measurement of protrusive forces generated by cells, in which cells spreading on a planar surface encounter an array of pillars. The protrusive force can be calculated from the deflection of the pillar using simple mechanical considerations. The advantage of this approach is that multiple sensors can be integrated into a single device. Theaverage protrusive force was calculated as 800 pN for NIH 3T3 fibroblasts. The correct quantification of protrusive forces potentially can be used in the development of novel cancer therapies and diagnostic tools.

Original language	English (US)
Article number	06FA02
Journal	Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics
Volume	29
Issue number	6
DOIs	https://doi.org/10.1116/1.3655580
State	Published - Nov 2011
Externally published	Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Process Chemistry and Technology
Surfaces, Coatings and Films
Electrical and Electronic Engineering
Materials Chemistry

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title = "New approach for measuring protrusive forces in cells",

abstract = "Forces experienced by cells internally and externally can alter cellular signaling and function. The authors have developed an elastomeric device for the measurement of protrusive forces generated by cells, in which cells spreading on a planar surface encounter an array of pillars. The protrusive force can be calculated from the deflection of the pillar using simple mechanical considerations. The advantage of this approach is that multiple sensors can be integrated into a single device. Theaverage protrusive force was calculated as 800 pN for NIH 3T3 fibroblasts. The correct quantification of protrusive forces potentially can be used in the development of novel cancer therapies and diagnostic tools.",

author = "A. Mathur and P. Roca-Cusachs and Rossier, {O. M.} and Wind, {S. J.} and Sheetz, {M. P.} and J. Hone",

note = "Funding Information: This work was funded by the National Institutes of Health (NIH) Common Fund Nanomedicine program (PN2 EY016586). The fabrication work was performed at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant No. ECS-0335765).",

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AU - Mathur, A.

AU - Roca-Cusachs, P.

AU - Rossier, O. M.

AU - Wind, S. J.

AU - Sheetz, M. P.

AU - Hone, J.

N1 - Funding Information: This work was funded by the National Institutes of Health (NIH) Common Fund Nanomedicine program (PN2 EY016586). The fabrication work was performed at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant No. ECS-0335765).

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N2 - Forces experienced by cells internally and externally can alter cellular signaling and function. The authors have developed an elastomeric device for the measurement of protrusive forces generated by cells, in which cells spreading on a planar surface encounter an array of pillars. The protrusive force can be calculated from the deflection of the pillar using simple mechanical considerations. The advantage of this approach is that multiple sensors can be integrated into a single device. Theaverage protrusive force was calculated as 800 pN for NIH 3T3 fibroblasts. The correct quantification of protrusive forces potentially can be used in the development of novel cancer therapies and diagnostic tools.

AB - Forces experienced by cells internally and externally can alter cellular signaling and function. The authors have developed an elastomeric device for the measurement of protrusive forces generated by cells, in which cells spreading on a planar surface encounter an array of pillars. The protrusive force can be calculated from the deflection of the pillar using simple mechanical considerations. The advantage of this approach is that multiple sensors can be integrated into a single device. Theaverage protrusive force was calculated as 800 pN for NIH 3T3 fibroblasts. The correct quantification of protrusive forces potentially can be used in the development of novel cancer therapies and diagnostic tools.

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New approach for measuring protrusive forces in cells

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