New separation tools for comprehensive studies of protein expression by mass spectrometry

Carol L. Nilsson, Pia Davidsson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (>150 kDa) and small (<10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.

Original languageEnglish (US)
Pages (from-to)390-397
Number of pages8
JournalMass Spectrometry Reviews
Volume19
Issue number6
StatePublished - Nov 2000
Externally publishedYes

Fingerprint

Mass spectrometry
Mass Spectrometry
mass spectroscopy
proteins
Proteins
Electrophoresis, Gel, Two-Dimensional
Biological systems
Peptide Mapping
gels
Computational Biology
Proteolysis
Membrane Proteins
Bioinformatics
electrophoresis
Electrophoresis
Gels
peptides
degradation
membranes
Membranes

Keywords

  • Liquid chromatography
  • Liquid phase electrophoresis
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Spectroscopy

Cite this

New separation tools for comprehensive studies of protein expression by mass spectrometry. / Nilsson, Carol L.; Davidsson, Pia.

In: Mass Spectrometry Reviews, Vol. 19, No. 6, 11.2000, p. 390-397.

Research output: Contribution to journalArticle

Nilsson, Carol L. ; Davidsson, Pia. / New separation tools for comprehensive studies of protein expression by mass spectrometry. In: Mass Spectrometry Reviews. 2000 ; Vol. 19, No. 6. pp. 390-397.
@article{ceb430d60061401a9f2b30db3f1fa02e,
title = "New separation tools for comprehensive studies of protein expression by mass spectrometry",
abstract = "Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (>150 kDa) and small (<10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.",
keywords = "Liquid chromatography, Liquid phase electrophoresis, Mass spectrometry, Proteomics",
author = "Nilsson, {Carol L.} and Pia Davidsson",
year = "2000",
month = "11",
language = "English (US)",
volume = "19",
pages = "390--397",
journal = "Mass Spectrometry Reviews",
issn = "0277-7037",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - New separation tools for comprehensive studies of protein expression by mass spectrometry

AU - Nilsson, Carol L.

AU - Davidsson, Pia

PY - 2000/11

Y1 - 2000/11

N2 - Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (>150 kDa) and small (<10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.

AB - Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (>150 kDa) and small (<10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.

KW - Liquid chromatography

KW - Liquid phase electrophoresis

KW - Mass spectrometry

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=0034326966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034326966&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 390

EP - 397

JO - Mass Spectrometry Reviews

JF - Mass Spectrometry Reviews

SN - 0277-7037

IS - 6

ER -