New technique for performing bronchoalveolar lavage in sheep

Clare Savage, Joseph B. Zwischenberger, Donald J. Deyo, Thomas A. Heming, Scott K. Alpard, Akhil Bidani

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Inflammatory changes in acute respiratory distress syndrome (ARDS) were evaluated using bronchoalveolar lavage (BAL) to sample inflammatory cells of the lower respiratory tract. Currently, no reliable technique exists for harvesting large numbers of viable alveolar macrophages. We constructed a modified bronchoalveolar lavage (MBAL) cannula using a commercially available retrograde coronary sinus reperfusion cannula with a large central lumen for rapid fluid instillation, a distal balloon for bronchial seal, and distal side ports to prevent a high-pressure jet. In normal sheep, mean aspirate return was significantly higher using MBAL compared with bronchoscopy (69 ± 4 mL vs. 25 ± 1 mL; p ≤ 0.001). We then compared macrophage yield using bronchoscopy and MBAL using their lethal dose 40% (LD40) smoke/burn sheep model of ARDS. Total macrophage recovery was significantly higher (more than 80 times) using MBAL compared with bronchoscopy (16.9 ± 3.6 × 106 macrophages vs. 0.20 ± 0.06 × 106 macrophages; p ≤ 0.009). Likewise, the number of macrophages per milliliter of aspirate was significantly higher with MBAL compared with bronchoscopy (0.46 ± 0.16 × 106 macrophages/mL vs. 0.009 ± 0.003 × 106 macrophages/mL; p ≤ 0.05). We conclude the MBAL cannula enables harvest of significantly higher quantities of macrophages in smoke/burn-injured sheep, facilitating serial assessment of the pathophysiology of ARDS.

    Original languageEnglish (US)
    Pages (from-to)17-20
    Number of pages4
    JournalJournal of Bronchology
    Volume8
    Issue number1
    DOIs
    StatePublished - 2001

    Fingerprint

    Bronchoalveolar Lavage
    Sheep
    Macrophages
    Bronchoscopy
    Adult Respiratory Distress Syndrome
    Burns
    Smoke
    Myocardial Reperfusion
    Coronary Sinus
    Alveolar Macrophages
    Respiratory System
    Pressure
    Cannula

    Keywords

    • ARDS
    • Bronchoalveolar lavage
    • Flexible bronchoscopy
    • Macrophage
    • Sheep

    ASJC Scopus subject areas

    • Pulmonary and Respiratory Medicine

    Cite this

    Savage, C., Zwischenberger, J. B., Deyo, D. J., Heming, T. A., Alpard, S. K., & Bidani, A. (2001). New technique for performing bronchoalveolar lavage in sheep. Journal of Bronchology, 8(1), 17-20. https://doi.org/10.1097/01.lbr.0000011070.41751.f7

    New technique for performing bronchoalveolar lavage in sheep. / Savage, Clare; Zwischenberger, Joseph B.; Deyo, Donald J.; Heming, Thomas A.; Alpard, Scott K.; Bidani, Akhil.

    In: Journal of Bronchology, Vol. 8, No. 1, 2001, p. 17-20.

    Research output: Contribution to journalArticle

    Savage, C, Zwischenberger, JB, Deyo, DJ, Heming, TA, Alpard, SK & Bidani, A 2001, 'New technique for performing bronchoalveolar lavage in sheep', Journal of Bronchology, vol. 8, no. 1, pp. 17-20. https://doi.org/10.1097/01.lbr.0000011070.41751.f7
    Savage C, Zwischenberger JB, Deyo DJ, Heming TA, Alpard SK, Bidani A. New technique for performing bronchoalveolar lavage in sheep. Journal of Bronchology. 2001;8(1):17-20. https://doi.org/10.1097/01.lbr.0000011070.41751.f7
    Savage, Clare ; Zwischenberger, Joseph B. ; Deyo, Donald J. ; Heming, Thomas A. ; Alpard, Scott K. ; Bidani, Akhil. / New technique for performing bronchoalveolar lavage in sheep. In: Journal of Bronchology. 2001 ; Vol. 8, No. 1. pp. 17-20.
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