Newly recognized hantaviruses associated with hantavirus pulmonary syndrome in Northern Brazil: Partial genetic characterization of viruses and serologic implication of likely reservoirs

Elizabeth S T Rosa, James N. Mills, Paula J. Padula, Mauro R. Elkhoury, Thomas Ksiazek, Wellington S. Mendes, Elizabeth D. Santos, Gisele C B Araújo, Valeria P. Martinez, Jorge F S T Rosa, Alexis Edelstein, Pedro Fernando da Costa Vasconcelos

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Following the occurrence of the first laboratory-confirmed cases of hantavirus pulmonary syndrome (HPS) in Maranhão State, Brazil, rodents were trapped and rodent materials screened by ELISA for antibodies to Sin Nombre and Andes hantaviruses. Antibody-positive samples were tested by RT-PCR, amplified products were sequenced, and phylogenetic trees were constructed for comparison with known hantaviruses. From 104 rodent blood samples collected (40 Bolomys lasiurus, 52 Holochilus sciureus, 12 Oligoryzomys fornesi, and one Proechimys guyannensis), 21 (20.2%) were antibody-positive (one B. lasiurus, five O. fornesi, and 15 H. sciureus). Hantavirus RNA was amplified by PCR from two O. fornesi and four H. sciureus. Viral sequencing identified two hantavirus genotypes. The genotype recovered from O. fornesi, is designated herein as Anajatuba (ANAJ) and the genotype recovered from H. sciureus is designated Rio Mearim (RIME). Phylogenetic analysis of a 643-nucleotide region of the N segment showed both viruses to be most closely related (94-96% nucleotide homology) to Río Mamoré virus, a virus associated with Oligoryzomys microtis in Bolivia and Peru, but not found in northern Brazil. O. fornesi was frequently captured in and around human dwellings. H. sciureus, is a semi-aquatic rodent captured only in remote areas rarely frequented by humans.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalVector-Borne and Zoonotic Diseases
Volume5
Issue number1
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Hantavirus Pulmonary Syndrome
Hantavirus
Brazil
Rodentia
Viruses
Genotype
Antibodies
Nucleotides
Bolivia
Polymerase Chain Reaction
Arvicolinae
Peru
Enzyme-Linked Immunosorbent Assay
RNA

Keywords

  • Anajatuba virus
  • Brazil
  • Hantaviruses
  • Holochilus sciureus
  • HPS
  • Oligoryzomys fornesi
  • Phylogenetic characterization
  • Rio Mearim virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Newly recognized hantaviruses associated with hantavirus pulmonary syndrome in Northern Brazil : Partial genetic characterization of viruses and serologic implication of likely reservoirs. / Rosa, Elizabeth S T; Mills, James N.; Padula, Paula J.; Elkhoury, Mauro R.; Ksiazek, Thomas; Mendes, Wellington S.; Santos, Elizabeth D.; Araújo, Gisele C B; Martinez, Valeria P.; Rosa, Jorge F S T; Edelstein, Alexis; Vasconcelos, Pedro Fernando da Costa.

In: Vector-Borne and Zoonotic Diseases, Vol. 5, No. 1, 03.2005, p. 11-19.

Research output: Contribution to journalArticle

Rosa, EST, Mills, JN, Padula, PJ, Elkhoury, MR, Ksiazek, T, Mendes, WS, Santos, ED, Araújo, GCB, Martinez, VP, Rosa, JFST, Edelstein, A & Vasconcelos, PFDC 2005, 'Newly recognized hantaviruses associated with hantavirus pulmonary syndrome in Northern Brazil: Partial genetic characterization of viruses and serologic implication of likely reservoirs', Vector-Borne and Zoonotic Diseases, vol. 5, no. 1, pp. 11-19. https://doi.org/10.1089/vbz.2005.5.11
Rosa, Elizabeth S T ; Mills, James N. ; Padula, Paula J. ; Elkhoury, Mauro R. ; Ksiazek, Thomas ; Mendes, Wellington S. ; Santos, Elizabeth D. ; Araújo, Gisele C B ; Martinez, Valeria P. ; Rosa, Jorge F S T ; Edelstein, Alexis ; Vasconcelos, Pedro Fernando da Costa. / Newly recognized hantaviruses associated with hantavirus pulmonary syndrome in Northern Brazil : Partial genetic characterization of viruses and serologic implication of likely reservoirs. In: Vector-Borne and Zoonotic Diseases. 2005 ; Vol. 5, No. 1. pp. 11-19.
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abstract = "Following the occurrence of the first laboratory-confirmed cases of hantavirus pulmonary syndrome (HPS) in Maranh{\~a}o State, Brazil, rodents were trapped and rodent materials screened by ELISA for antibodies to Sin Nombre and Andes hantaviruses. Antibody-positive samples were tested by RT-PCR, amplified products were sequenced, and phylogenetic trees were constructed for comparison with known hantaviruses. From 104 rodent blood samples collected (40 Bolomys lasiurus, 52 Holochilus sciureus, 12 Oligoryzomys fornesi, and one Proechimys guyannensis), 21 (20.2{\%}) were antibody-positive (one B. lasiurus, five O. fornesi, and 15 H. sciureus). Hantavirus RNA was amplified by PCR from two O. fornesi and four H. sciureus. Viral sequencing identified two hantavirus genotypes. The genotype recovered from O. fornesi, is designated herein as Anajatuba (ANAJ) and the genotype recovered from H. sciureus is designated Rio Mearim (RIME). Phylogenetic analysis of a 643-nucleotide region of the N segment showed both viruses to be most closely related (94-96{\%} nucleotide homology) to R{\'i}o Mamor{\'e} virus, a virus associated with Oligoryzomys microtis in Bolivia and Peru, but not found in northern Brazil. O. fornesi was frequently captured in and around human dwellings. H. sciureus, is a semi-aquatic rodent captured only in remote areas rarely frequented by humans.",
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