NF-κB activation by oxidative stress and inflammation suppresses contractility in colonic circular smooth muscle cells

Xuan-Zheng Shi, Paul F. Lindholm, Sushil K. Sarna

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Background & Aims: Transcription factor nuclear factor κB (NF-κB) plays a critical role in transcriptional changes in several diseases, including inflammation. The aim of this study was to investigate whether NF-κB is activated by inflammation and oxidative stress in colonic circular smooth muscle cells and whether that leads to suppression of their contractility. Methods: The experiments were performed on freshly dissociated single cells using electrophoretic mobility shift assay, Western immunoblotting, and immunofluorescence imaging. Results: The NF-κB DNA binding was ∼6-fold greater in cells from the inflamed colon vs. those from the normal colon. Supershift assay indicated that the antibodies to p65, p50, and c-Rel, but not that to p52, shifted the NF-κB band. Western immunoblotting and immunofluorescence imaging also demonstrated the presence of p65, p50, and c-Rel proteins in the cytoplasm and their translocation to the nucleus by H2O2-induced oxidative stress. H2O2 treatment degraded IκBβ, but not IκBα to translocate NF-κB to the nucleus. Hydrogen peroxide concentration and time dependently activated NF-κB DNA binding and suppressed cell contraction to acetylcholine. NF-κB inhibitors significantly inhibited these effects. Inhibition of NF-κB prior to and during inflammation in intact dogs also reversed the suppression of contractility. Conclusions: Transcription factor NF-κB is activated in colonic circular muscle cells by inflammation and oxidative stress. This activation of NF-κB mediates the suppression of cell contractility.

Original languageEnglish (US)
Pages (from-to)1369-1380
Number of pages12
JournalGastroenterology
Volume124
Issue number5
DOIs
StatePublished - May 1 2003

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Smooth Muscle Myocytes
Oxidative Stress
Inflammation
Fluorescent Antibody Technique
Proto-Oncogene Proteins c-rel
Colon
Transcription Factors
Western Blotting
DNA
Electrophoretic Mobility Shift Assay
Muscle Cells
Hydrogen Peroxide
Acetylcholine
Cytoplasm
Dogs
Antibodies
Therapeutics

ASJC Scopus subject areas

  • Gastroenterology

Cite this

NF-κB activation by oxidative stress and inflammation suppresses contractility in colonic circular smooth muscle cells. / Shi, Xuan-Zheng; Lindholm, Paul F.; Sarna, Sushil K.

In: Gastroenterology, Vol. 124, No. 5, 01.05.2003, p. 1369-1380.

Research output: Contribution to journalArticle

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