NF-κB-inducible BCL-3 Expression is an Autoregulatory Loop Controlling Nuclear p50/NF-κB1 Residence

Allan R. Brasier, Muping Lu, Tao Hai, Ying Lu, Istvan Boldogh

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

NF-κB is a transcription factor whose nuclear residence is controlled by IκB family members. In the NF-κB-IκB autoregulatory loop, activated (nuclear) Rel A·NF-κB1 induces the resynthesis of IκBα recapturing nuclear Rel A back into the cytoplasm within 1 h of stimulation. In contrast, NF-κB1 subunits redistribute more slowly into the cytoplasm (from 6 to 12 h). Here we examine the role of inducible cytoplasmic BCL-3 expression in terminating nuclear NF-κB1. Although BCL-3 is a nuclear protein in B lymphocytes, surprisingly, BCL-3 is primarily a cytoplasmic protein in HepG2 cells. Cytoplasmic BCL-3 abundance is induced 6-12 h after tumor necrosis factor-α stimulation where it complexes with NF-κB1 homodimers. Moreover, BCL-3 mRNA and protein expression are induced by NF-κ B-activating agents. Two observations are interpreted to indicate that bcl-3 is transactivated by NF-κB/Rel A:1) expression of a dominant negative NF-κB inhibitor blocks tumor necrosis factor-α-induced BCL-3 expression and 2) expression of constitutively active Rel A is sufficient to induce BCL-3 expression. In gene transfer studies, we identify two high affinity NF-κB-binding sites, κB1 (located at -872 to -861 nucleotides) and κB2 (-106 to -96 nucleotides), and although both bind with high affinity to Rel A, only κB2 is required for NF-κ B-dependent induction of the native BCL-3 promoter. Down-regulation of BCL-3 induction results in prolonged, enhanced NF-κB1 binding and increased NF-κB-dependent transcription. Together, these data suggest the presence of an NF-κB-BCL-3 autoregulatory loop important in terminating NF-κB1 action and that individual NF-κB isoforms are actively terminated through coordinate induction of inhibitory IκB molecules to restore cellular homeostasis.

Original languageEnglish (US)
Pages (from-to)32080-32093
Number of pages14
JournalJournal of Biological Chemistry
Volume276
Issue number34
DOIs
StatePublished - Aug 24 2001

Fingerprint

Cytoplasm
Nucleotides
Tumor Necrosis Factor-alpha
Gene transfer
Lymphocytes
Hep G2 Cells
Atrial Natriuretic Factor
Transcription
Nuclear Proteins
Protein Isoforms
Proteins
Homeostasis
B-Lymphocytes
Transcription Factors
Down-Regulation
Binding Sites
Messenger RNA
Molecules
Genes
IgA receptor

ASJC Scopus subject areas

  • Biochemistry

Cite this

NF-κB-inducible BCL-3 Expression is an Autoregulatory Loop Controlling Nuclear p50/NF-κB1 Residence. / Brasier, Allan R.; Lu, Muping; Hai, Tao; Lu, Ying; Boldogh, Istvan.

In: Journal of Biological Chemistry, Vol. 276, No. 34, 24.08.2001, p. 32080-32093.

Research output: Contribution to journalArticle

Brasier, Allan R. ; Lu, Muping ; Hai, Tao ; Lu, Ying ; Boldogh, Istvan. / NF-κB-inducible BCL-3 Expression is an Autoregulatory Loop Controlling Nuclear p50/NF-κB1 Residence. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 34. pp. 32080-32093.
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