NGF-producing transfected 3T3 cells

Behavioral and histological assessment of transplants in nigral lesioned rats

K. N. Westlund, Y. Lu, M. Kadekaro, P. Harmann, M. L. Terrell, D. P. Pizzo, C. E. Hulsebosch, H. M. Eisenberg, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The rodent fibroblast clonal cell line, 3T3, was retrovirally transfected with the rat nerve growth factor (NGF) gene and selected for NGF synthesis. This study tested the hypothesis that transplanted 3T3 cells, transfected to secrete nerve growth factor (3T3(NGF+)), change motor behavioral indices created by striatal denervation in a dose-dependent fashion. 3T3(NGF+) cells were transplanted into the lateral ventricle of rats following ipsilateral lesions of the substantia nigra pars compacta by stereotaxic injections of 6- hydroxydopamine (10 μg), an established lesion model. Control groups included vehicle injections and transplanted untransfected cells. The extent of the lesions was measured by determining rotational behavior before and two weeks after transplantation. Immediately prior to transplantation, cells were incubated with the fluorescent dye marker, Dil. To assess cell viability, whole brains were cryosectioned and examined for Dil-labeled 3T3 cells using fluorescent microscopy. The number of Dil-labeled profiles in five animals per group were counted in at least five noncontiguous sections per animal. From these data a statistically derived estimate of viable, transplanted 3T3 cells was obtained. The number of surviving transplanted cells correlated with the behavioral changes measured. The 3T3(NGF+) transplants reduced rotational behavior, while control 3T3 transplants exacerbated rotational behavior. Thus, while NGF delivery was found to be beneficial, it was apparent that naive 3T3 had detrimental effects. These results underscore the importance of making dose-response measurements when attempting transplant- based modifications of CNS behavior.

    Original languageEnglish (US)
    Pages (from-to)367-373
    Number of pages7
    JournalJournal of Neuroscience Research
    Volume41
    Issue number3
    DOIs
    StatePublished - 1995

    Fingerprint

    3T3 Cells
    Nerve Growth Factor
    Substantia Nigra
    Transplants
    Corpus Striatum
    Injections
    Behavior Control
    Behavior Therapy
    Lateral Ventricles
    Oxidopamine
    Cell Transplantation
    Denervation
    Fluorescent Dyes
    Microscopy
    Rodentia
    Cell Survival
    Fibroblasts
    Transplantation
    Cell Line
    Control Groups

    Keywords

    • 6- hydroxydopamine
    • nerve growth factor
    • rotational behavior
    • striatum
    • substantia nigra
    • transplant

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Westlund, K. N., Lu, Y., Kadekaro, M., Harmann, P., Terrell, M. L., Pizzo, D. P., ... Perez-Polo, J. R. (1995). NGF-producing transfected 3T3 cells: Behavioral and histological assessment of transplants in nigral lesioned rats. Journal of Neuroscience Research, 41(3), 367-373. https://doi.org/10.1002/jnr.490410309

    NGF-producing transfected 3T3 cells : Behavioral and histological assessment of transplants in nigral lesioned rats. / Westlund, K. N.; Lu, Y.; Kadekaro, M.; Harmann, P.; Terrell, M. L.; Pizzo, D. P.; Hulsebosch, C. E.; Eisenberg, H. M.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 41, No. 3, 1995, p. 367-373.

    Research output: Contribution to journalArticle

    Westlund, KN, Lu, Y, Kadekaro, M, Harmann, P, Terrell, ML, Pizzo, DP, Hulsebosch, CE, Eisenberg, HM & Perez-Polo, JR 1995, 'NGF-producing transfected 3T3 cells: Behavioral and histological assessment of transplants in nigral lesioned rats', Journal of Neuroscience Research, vol. 41, no. 3, pp. 367-373. https://doi.org/10.1002/jnr.490410309
    Westlund, K. N. ; Lu, Y. ; Kadekaro, M. ; Harmann, P. ; Terrell, M. L. ; Pizzo, D. P. ; Hulsebosch, C. E. ; Eisenberg, H. M. ; Perez-Polo, J. R. / NGF-producing transfected 3T3 cells : Behavioral and histological assessment of transplants in nigral lesioned rats. In: Journal of Neuroscience Research. 1995 ; Vol. 41, No. 3. pp. 367-373.
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    AU - Terrell, M. L.

    AU - Pizzo, D. P.

    AU - Hulsebosch, C. E.

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