NH2 terminus of PTB-associated splicing factor binds to the porcine P450scc IGF-I response element

Randall Urban, Yvonne H. Bodenburg, Thomas Wood

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An insulin-like growth factor (IGF) I response element (IGFRE) in the porcine P-450 cholesterol side-chain cleavage gene (P450scc) regulates transcription through the binding of two proteins, Sp1 and polypyrimidine tract-binding protein-associated splicing factor (PSF). PSF is a component of spliceosomes and contains RNA-binding domains. In this study, we localized the NH2-terminal amino acid residues necessary for binding of PSF to the IGFRE. Three COOH-terminal truncated proteins (aa 304, 214, and 134) of PSF were designed to empirically partition the NH2-terminal region while excluding the RNA-binding domains. Southwestern analysis showed that only the largest expressed truncated protein, P3, strongly bound the porcine P450scc IGFRE. Truncated PSF protein expression in Y1 adrenal cells showed that P3 repressed transcriptional activity of the IGFRE similar to full-length PSF, whereas P2 (minimal binding to the IGFRE) had no effect. In conclusion, the NH2-terminal region of PSF contains the amino acid residues necessary for binding to the porcine P450scc IGFRE and repressing the transcriptional activity of the element.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume283
Issue number3 46-3
StatePublished - Sep 1 2002

Fingerprint

Protein Splicing
Response Elements
Insulin-Like Growth Factor I
Swine
Genes
Cholesterol
Proteins
Carrier Proteins
Spliceosomes
Amino Acids
Polypyrimidine Tract-Binding Protein
RNA
PTB-Associated Splicing Factor
RNA Splicing Factors
Transcription

Keywords

  • Insulin-like growth factor-I
  • P-450 cholesterol side-chain cleavage
  • Polypyrimidine tract-binding protein
  • Sp1
  • Steroidogenesis
  • Transcription

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

NH2 terminus of PTB-associated splicing factor binds to the porcine P450scc IGF-I response element. / Urban, Randall; Bodenburg, Yvonne H.; Wood, Thomas.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 283, No. 3 46-3, 01.09.2002.

Research output: Contribution to journalArticle

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